The social nutrition status was investigated among 246 subjects aged 60-90 living at three urban communities in Chengdu of Sichuan Province. The questionnaire was designed to evaluate socirydeweraphic background, the ...The social nutrition status was investigated among 246 subjects aged 60-90 living at three urban communities in Chengdu of Sichuan Province. The questionnaire was designed to evaluate socirydeweraphic background, the subjects' nutrition knowledge, and the support systerns for geriatric nutrition. Fasting venous blcod was colected for the analysis of biochemical parameters. Blood pressure, bene mineral contents (BMC), body weight (BW) and they height (BH) were measured at the same time. Only 49. 7% of the subjects correctly an swered four basic questions on nutrition. Food patterns for the elder1y were simple and modest. Several nutrition-related disorders for the elderly were including high systolic bbo pressure (44. 6 % ), hyPertriglyceridemia (25. 9 % ), high diasto1ic blood pressure (25. 1% ),obesity (24. 5% ), high PBG (20. 6% ), emaciation (19. 9%), high FBG (17.9%) and osteoporosis (16. 8%). These data indicate that the support systems for the geriatric nutrition will have to be improved.展开更多
Ra ostcobasts were isolated from the 21-day fetal rat calvchas. The cells were grown in DMEM Plus 10% FBS, and were treated for 24 h. with 10 μmol/L TPEN or 10 μmol/L TPEN supplemented with 10 μmol/L Zn2+. Apoptos...Ra ostcobasts were isolated from the 21-day fetal rat calvchas. The cells were grown in DMEM Plus 10% FBS, and were treated for 24 h. with 10 μmol/L TPEN or 10 μmol/L TPEN supplemented with 10 μmol/L Zn2+. Apoptosis of osteoblasts were measured by fiow cytometry, electron microscopy and DNA fragmentation analyzed by gel elecmphoresis. In addition, IP3 production and PKC activity were measmed in ordr to show whether they are involved in apoptosis in osteoblast induced by alnc deficiency. The results showed that 10 μmol/L TPEN could induce apoptosis in osteoblast in 24 h. But cells ed with 10μmol/L TPEN supplemented with 10 μmol/L Zn2+ showed no apoptotic changs in 24 h. TPEN significantly reduced the formation of IP3 and PKC activity after 24 h incubation. No differences were observed between the cells treated with TPEN supPlemented with Zn2+ simulaneosly and the untreated cells. It can be inferred that apoptosis induced by ainc deficiency may be due to the decreased activity of PKC which is impaired by reduced formation of IP3.展开更多
文摘The social nutrition status was investigated among 246 subjects aged 60-90 living at three urban communities in Chengdu of Sichuan Province. The questionnaire was designed to evaluate socirydeweraphic background, the subjects' nutrition knowledge, and the support systerns for geriatric nutrition. Fasting venous blcod was colected for the analysis of biochemical parameters. Blood pressure, bene mineral contents (BMC), body weight (BW) and they height (BH) were measured at the same time. Only 49. 7% of the subjects correctly an swered four basic questions on nutrition. Food patterns for the elder1y were simple and modest. Several nutrition-related disorders for the elderly were including high systolic bbo pressure (44. 6 % ), hyPertriglyceridemia (25. 9 % ), high diasto1ic blood pressure (25. 1% ),obesity (24. 5% ), high PBG (20. 6% ), emaciation (19. 9%), high FBG (17.9%) and osteoporosis (16. 8%). These data indicate that the support systems for the geriatric nutrition will have to be improved.
文摘Ra ostcobasts were isolated from the 21-day fetal rat calvchas. The cells were grown in DMEM Plus 10% FBS, and were treated for 24 h. with 10 μmol/L TPEN or 10 μmol/L TPEN supplemented with 10 μmol/L Zn2+. Apoptosis of osteoblasts were measured by fiow cytometry, electron microscopy and DNA fragmentation analyzed by gel elecmphoresis. In addition, IP3 production and PKC activity were measmed in ordr to show whether they are involved in apoptosis in osteoblast induced by alnc deficiency. The results showed that 10 μmol/L TPEN could induce apoptosis in osteoblast in 24 h. But cells ed with 10μmol/L TPEN supplemented with 10 μmol/L Zn2+ showed no apoptotic changs in 24 h. TPEN significantly reduced the formation of IP3 and PKC activity after 24 h incubation. No differences were observed between the cells treated with TPEN supPlemented with Zn2+ simulaneosly and the untreated cells. It can be inferred that apoptosis induced by ainc deficiency may be due to the decreased activity of PKC which is impaired by reduced formation of IP3.
