Although the phosphate 1（PHO1）gene family has been implicated in inorganic phosphate transport and homeostasis,the underlying mechanism of this gene in the strawberry has not yet been revealed.In the present study,w...Although the phosphate 1（PHO1）gene family has been implicated in inorganic phosphate transport and homeostasis,the underlying mechanism of this gene in the strawberry has not yet been revealed.In the present study,we analyzed the expression of the PHO1;H9 gene in the strawberry（Fragaria×ananassa）,revealing the involvement of this gene in the regulation of phosphorus（P）content.The coding sequence（CDS）of the PHO1;H9 gene,was isolated from the cultivated strawberry‘Sachinoka’and named as Fa PHO1;H9.The full-length CDS of this gene was 2 292 bp,encoding 763 amino acids,and the protein contained both SYG1/Pho81/XPR1（SPX）and ERD1/XPR1/SYG1（EXS）domains,which were involved in phosphate（Pi）signaling.Real-time reverse transcription-polymerase chain reaction（RT-PCR）data suggested that the level of Fa PHO1;H9 expression was consistent with the P content in different organs,except for the petiole.Particularly,its expression level was also correlated with P content in fruits of different developmental stages.The expression of Fa PHO1;H9 was also consistent with P content in leaves under different concentrations of P fertilizer application.Furthermore,transgenic Arabidopsis lines were generated,and the P content in Arabidopsis plants over-expressing Fa PHO1;H9was significantly higher than that in wild-type plants.Therefore,we proposed that Fa PHO1;H9 functions in P transport.展开更多
Genes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate （EMS）-mutagenized lines of Fragaria vesca accession Yellow Wonder （YW）, two fruit shapes are observed： wild-type long f...Genes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate （EMS）-mutagenized lines of Fragaria vesca accession Yellow Wonder （YW）, two fruit shapes are observed： wild-type long fruit and mutated shortened fruit （sf）. In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin （GA）-deficient mutant, and four complementary DNA （cDNA） libraries separately constructed from flower buds and small green fruits of YW and sfwere sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YWl and sfl, and 28 differentially expressed GA pathway genes were identified between YW2 and sf2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR （qRT-PCR）, and the results were highly concordant with the RNA-Seq results.＇ This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry.展开更多
基金financially supported by the National Natural Science Foundation of China (31372037)the Program for Excellent Talents in University of Liaoning Province, China (LJQ2014069)
文摘Although the phosphate 1（PHO1）gene family has been implicated in inorganic phosphate transport and homeostasis,the underlying mechanism of this gene in the strawberry has not yet been revealed.In the present study,we analyzed the expression of the PHO1;H9 gene in the strawberry（Fragaria×ananassa）,revealing the involvement of this gene in the regulation of phosphorus（P）content.The coding sequence（CDS）of the PHO1;H9 gene,was isolated from the cultivated strawberry‘Sachinoka’and named as Fa PHO1;H9.The full-length CDS of this gene was 2 292 bp,encoding 763 amino acids,and the protein contained both SYG1/Pho81/XPR1（SPX）and ERD1/XPR1/SYG1（EXS）domains,which were involved in phosphate（Pi）signaling.Real-time reverse transcription-polymerase chain reaction（RT-PCR）data suggested that the level of Fa PHO1;H9 expression was consistent with the P content in different organs,except for the petiole.Particularly,its expression level was also correlated with P content in fruits of different developmental stages.The expression of Fa PHO1;H9 was also consistent with P content in leaves under different concentrations of P fertilizer application.Furthermore,transgenic Arabidopsis lines were generated,and the P content in Arabidopsis plants over-expressing Fa PHO1;H9was significantly higher than that in wild-type plants.Therefore,we proposed that Fa PHO1;H9 functions in P transport.
基金supported by the National Natural Science Foundation of China(31372037)
文摘Genes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate （EMS）-mutagenized lines of Fragaria vesca accession Yellow Wonder （YW）, two fruit shapes are observed： wild-type long fruit and mutated shortened fruit （sf）. In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin （GA）-deficient mutant, and four complementary DNA （cDNA） libraries separately constructed from flower buds and small green fruits of YW and sfwere sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YWl and sfl, and 28 differentially expressed GA pathway genes were identified between YW2 and sf2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR （qRT-PCR）, and the results were highly concordant with the RNA-Seq results.＇ This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry.
