目的:探讨C3扩大半椎板切除单开门椎板成形术治疗多节段颈脊髓压迫症的临床疗效。方法:对2014年9月至2018年5月收治的58例多节段颈脊髓压迫症患者进行回顾性研究,其中男34例,女24例;年龄46~78岁,平均64.4岁;28例采用C3扩大半椎板切除C4...目的:探讨C3扩大半椎板切除单开门椎板成形术治疗多节段颈脊髓压迫症的临床疗效。方法:对2014年9月至2018年5月收治的58例多节段颈脊髓压迫症患者进行回顾性研究,其中男34例,女24例;年龄46~78岁,平均64.4岁;28例采用C3扩大半椎板切除C4-C7单开门椎板成形术(改良开门组),30例采用传统后路C3-C7单开门椎板成形术治疗(传统开门组)。比较两组患者的手术时间、术中出血、C5神经麻痹及轴性症状(axial symptoms,AS)的发生情况;采用颈椎MRI横断面测量C3节段最狭窄处(包括C3,4椎间盘水平)脊髓有效空间(space available for the spinal cord,SAC),评价影像学指标改善情况。观察两组患者手术前后的日本骨科学会(Japanese Orthopaedic Association,JOA)评分、颈部功能障碍指数(Neck Disability Index,NDI),并计算神经功能改善率(RR)。结果:两组患者术后均获得随访,时间12~18个月,其中改良开门组平均随访时间为(14.5±1.8)个月,传统开门组为(14.5±1.9)个月,两组比较差异无统计学意义(P>0.05)。两组患者在术中出血、C5神经麻痹方面差异均无统计学意义(P>0.05);但手术时间[(119±10)minVS(126±12)min]、轴性症状发生率[7.1%(2/28)vs 26.6%(8/30)],差异有统计学意义(P<0.05)。改良开门组患者手术前后C3水平脊髓有效空间分别为(93.61±9.02)mm3和(153.5±12.76)mm3,术后较术前明显扩大(P<0.05);末次随访时改良开门组和传统开门组的JOA评分分别为14.36±1.70和14.03±1.82,NDI评分分别为10.36±2.55和12.47±3.46,两组差异有统计学意义(P<0.05),但两组神经功能改善率[(68.36±0.12)%VS(65.01±0.12)%]比较差异无统计学意义(P>0.05)。结论:C3扩大半椎板切除单开门椎板成形术不仅能够充分地解除脊髓压迫,而且通过减少C2棘突肌肉止点的剥离,在预防轴性症状等并发症方面亦取得了良好的效果,是一种治疗多节段颈脊髓压迫症的有效方法。展开更多
In order to realize real-time online monitoring of the wastewater source enterprises,manage and issue monitoring information,this paper comprehensively uses automatic control,embedded data acquisition and transmission...In order to realize real-time online monitoring of the wastewater source enterprises,manage and issue monitoring information,this paper comprehensively uses automatic control,embedded data acquisition and transmission,distributed computing and data processing,geographic information system,etc.to develop automatic monitoring system of the wastewater source in Shandong Province.This system incorporates automatic monitoring information acquisition,transmission and daily work as an organic whole.The system realizes not only the continuous online monitoring of wastewater source enterprise,but also the deep excavation and utilization on monitoring information.It provides scientific and objective basis for energy saving,consumption reduction,carbon emission reduction,total amount control and other environmental management works,and meets the requirements of environmental management and related departments to wastewater source management.展开更多
Background ^11C-4-N-（3-bromoanilino）-6,7-dimethoxyquinazoline （1^11C-PD153035） has been reported as a tracer for imaging human tumors that overexpress epidermal growth factor receptor （EGFR）. However it is still...Background ^11C-4-N-（3-bromoanilino）-6,7-dimethoxyquinazoline （1^11C-PD153035） has been reported as a tracer for imaging human tumors that overexpress epidermal growth factor receptor （EGFR）. However it is still unclear whether ^11C-PD153035 uptake correlates with EGFR expression levels. The objective of this study was to investigate the relationship between ^11C-PD153035 accumulation and EGFR expression levels. Methods Synthesis of ^11C-PD153035 was performed in the Tracerlab FXc system. Accumulation of ^11C-PD153035 by MDA-MB-468, A549 and MDA-MB-231 cells was measured in vitro. There were six tumor-bearing mice in each group. ^11C-PD153035 uptake in tumors was determined by positron emission tomography/computed tomography （PET/CT）. Tumor/normal muscle tissue （TINT） analysis in PET images was applied to quantify the PET data. Sixty minutes after PET/CT scanning, the nude mice were sacrificed and the tumors were excised. The ^11C-PD153035 accumulation in different tumors was determined by a gamma counter. Results Close correlation existed between the uptake and the level of EGFR expression both in vitro and ex vivo （r^2=0.72, P〈0.001; r^2=0.63, P=0.003）. When the static TINT analysis method was applied to analyze the PET data, the observed correlation was again excellent （^2=0.70, P=-0.001）. Conclusions The uptake of PET tracer ^11C-PD153035 closely correlates with the EGFR expression levels in tumor cells. ^11C-PD153035 has the potential to yield useful information for both cancer diagnosis and therapy.展开更多
Background Osteopontin （OPN） is a secreted phosphoglycoprotein （SSP） that is overexpressed in a variety of tumors and was regarded as a molecular marker of tumors.In this study,we intended to demonstrate the role ...Background Osteopontin （OPN） is a secreted phosphoglycoprotein （SSP） that is overexpressed in a variety of tumors and was regarded as a molecular marker of tumors.In this study,we intended to demonstrate the role of OPN in human breast cancer cell line MDA-MB-231.Methods Recombinant plasmid expressing small interfering RNA （siRNA） specific to OPN mRNA was transfected into MDA-MB-231 cells to generate the stable transfected cell line MDA-MB-343,and the empty plasmid tansfected cells （MDA-MB-neg） or wildtype MDA-MB-231 cells were used as control cells respectively.Expression of OPN,hypoxia inducible factor-1 （HIF-1） and vascular endothelial growth factor （VEGF） proteins was analyzed by Western blotting analysis.The radiosensitivity of cells was determined by detecting cell apoptosis,cell proliferation and cell senescence.Results HIF-1 and VEGF proteins in MDA-MB-343 cells were significantly downregulated upon the efficient knockdown of OPN expression under either hypoxia or normoxia environment.Moreover,expression of OPN protein was upregualted upon hypoxic culture.Stable OPN-silencing also decreased cell invasion,increased cell apoptosis and cell senescence,as well as reduced clonogenic survival,resulting in increase radiation tolerance.Conclusions Suppression of OPN gene expression can enhance radiosensitivity and affect cell apoptosis in breast cancer cells.OPN seems to be an attractive target for the improvement of radiotherapy.展开更多
文摘目的:探讨C3扩大半椎板切除单开门椎板成形术治疗多节段颈脊髓压迫症的临床疗效。方法:对2014年9月至2018年5月收治的58例多节段颈脊髓压迫症患者进行回顾性研究,其中男34例,女24例;年龄46~78岁,平均64.4岁;28例采用C3扩大半椎板切除C4-C7单开门椎板成形术(改良开门组),30例采用传统后路C3-C7单开门椎板成形术治疗(传统开门组)。比较两组患者的手术时间、术中出血、C5神经麻痹及轴性症状(axial symptoms,AS)的发生情况;采用颈椎MRI横断面测量C3节段最狭窄处(包括C3,4椎间盘水平)脊髓有效空间(space available for the spinal cord,SAC),评价影像学指标改善情况。观察两组患者手术前后的日本骨科学会(Japanese Orthopaedic Association,JOA)评分、颈部功能障碍指数(Neck Disability Index,NDI),并计算神经功能改善率(RR)。结果:两组患者术后均获得随访,时间12~18个月,其中改良开门组平均随访时间为(14.5±1.8)个月,传统开门组为(14.5±1.9)个月,两组比较差异无统计学意义(P>0.05)。两组患者在术中出血、C5神经麻痹方面差异均无统计学意义(P>0.05);但手术时间[(119±10)minVS(126±12)min]、轴性症状发生率[7.1%(2/28)vs 26.6%(8/30)],差异有统计学意义(P<0.05)。改良开门组患者手术前后C3水平脊髓有效空间分别为(93.61±9.02)mm3和(153.5±12.76)mm3,术后较术前明显扩大(P<0.05);末次随访时改良开门组和传统开门组的JOA评分分别为14.36±1.70和14.03±1.82,NDI评分分别为10.36±2.55和12.47±3.46,两组差异有统计学意义(P<0.05),但两组神经功能改善率[(68.36±0.12)%VS(65.01±0.12)%]比较差异无统计学意义(P>0.05)。结论:C3扩大半椎板切除单开门椎板成形术不仅能够充分地解除脊髓压迫,而且通过减少C2棘突肌肉止点的剥离,在预防轴性症状等并发症方面亦取得了良好的效果,是一种治疗多节段颈脊髓压迫症的有效方法。
基金Supported by Distributed Multilevel Environmental Automatic Monitoring Information Management SystemResearch on Automatic Monitoring Quality Management of the Water Pollution Source(2009ZX07527-002)
文摘In order to realize real-time online monitoring of the wastewater source enterprises,manage and issue monitoring information,this paper comprehensively uses automatic control,embedded data acquisition and transmission,distributed computing and data processing,geographic information system,etc.to develop automatic monitoring system of the wastewater source in Shandong Province.This system incorporates automatic monitoring information acquisition,transmission and daily work as an organic whole.The system realizes not only the continuous online monitoring of wastewater source enterprise,but also the deep excavation and utilization on monitoring information.It provides scientific and objective basis for energy saving,consumption reduction,carbon emission reduction,total amount control and other environmental management works,and meets the requirements of environmental management and related departments to wastewater source management.
文摘Background ^11C-4-N-（3-bromoanilino）-6,7-dimethoxyquinazoline （1^11C-PD153035） has been reported as a tracer for imaging human tumors that overexpress epidermal growth factor receptor （EGFR）. However it is still unclear whether ^11C-PD153035 uptake correlates with EGFR expression levels. The objective of this study was to investigate the relationship between ^11C-PD153035 accumulation and EGFR expression levels. Methods Synthesis of ^11C-PD153035 was performed in the Tracerlab FXc system. Accumulation of ^11C-PD153035 by MDA-MB-468, A549 and MDA-MB-231 cells was measured in vitro. There were six tumor-bearing mice in each group. ^11C-PD153035 uptake in tumors was determined by positron emission tomography/computed tomography （PET/CT）. Tumor/normal muscle tissue （TINT） analysis in PET images was applied to quantify the PET data. Sixty minutes after PET/CT scanning, the nude mice were sacrificed and the tumors were excised. The ^11C-PD153035 accumulation in different tumors was determined by a gamma counter. Results Close correlation existed between the uptake and the level of EGFR expression both in vitro and ex vivo （r^2=0.72, P〈0.001; r^2=0.63, P=0.003）. When the static TINT analysis method was applied to analyze the PET data, the observed correlation was again excellent （^2=0.70, P=-0.001）. Conclusions The uptake of PET tracer ^11C-PD153035 closely correlates with the EGFR expression levels in tumor cells. ^11C-PD153035 has the potential to yield useful information for both cancer diagnosis and therapy.
文摘Background Osteopontin （OPN） is a secreted phosphoglycoprotein （SSP） that is overexpressed in a variety of tumors and was regarded as a molecular marker of tumors.In this study,we intended to demonstrate the role of OPN in human breast cancer cell line MDA-MB-231.Methods Recombinant plasmid expressing small interfering RNA （siRNA） specific to OPN mRNA was transfected into MDA-MB-231 cells to generate the stable transfected cell line MDA-MB-343,and the empty plasmid tansfected cells （MDA-MB-neg） or wildtype MDA-MB-231 cells were used as control cells respectively.Expression of OPN,hypoxia inducible factor-1 （HIF-1） and vascular endothelial growth factor （VEGF） proteins was analyzed by Western blotting analysis.The radiosensitivity of cells was determined by detecting cell apoptosis,cell proliferation and cell senescence.Results HIF-1 and VEGF proteins in MDA-MB-343 cells were significantly downregulated upon the efficient knockdown of OPN expression under either hypoxia or normoxia environment.Moreover,expression of OPN protein was upregualted upon hypoxic culture.Stable OPN-silencing also decreased cell invasion,increased cell apoptosis and cell senescence,as well as reduced clonogenic survival,resulting in increase radiation tolerance.Conclusions Suppression of OPN gene expression can enhance radiosensitivity and affect cell apoptosis in breast cancer cells.OPN seems to be an attractive target for the improvement of radiotherapy.
