利用平板对峙实验及小区试验,筛选高效防治西洋参立枯病的木霉菌株,并采用ITS(internal transcribed spacer)序列分析与形态学特征相结合的方法进行种类鉴定。结果表明,供试木霉菌株在PDA平板上对立枯丝核菌( R. solani )均有抑制作用,...利用平板对峙实验及小区试验,筛选高效防治西洋参立枯病的木霉菌株,并采用ITS(internal transcribed spacer)序列分析与形态学特征相结合的方法进行种类鉴定。结果表明,供试木霉菌株在PDA平板上对立枯丝核菌( R. solani )均有抑制作用,菌株HB20111效果最好,抑制率达99.33%;菌株HB20111对西洋参立枯病的小区效果显著,与其他处理间存在显著性差异( P <0.05);第1年拌种处理对西洋参立枯病的防治效果、单株参增重率及总参增重率分别达71.81%、 33.54%和92.75%;第2年蘸根处理对西洋参立枯病的防治效果、单株参增重率及总参增重率分别达91.04%、158.06%和1 369.34%;菌株HB20111的分生孢子梗为典型的单侧分枝,其他形态学特征同深绿木霉( T. atroviride )一致,ITS序列与 T. atroviride 同源性达 100.0%,将菌株HB20111鉴定为 T. atroviride 。HB20111生长速度快,产孢能力强,生防效果高,有望替代化学杀菌剂应用于西洋参种植中。展开更多
Objective: To explore the effect of Kuanxiong Aerosol(KXA)on isoproterenol(ISO)-induced myocardial injury in rat models.Methods: Totally 24 rats were radomly divided into control,ISO,KXA low-dose and high-dose groups ...Objective: To explore the effect of Kuanxiong Aerosol(KXA)on isoproterenol(ISO)-induced myocardial injury in rat models.Methods: Totally 24 rats were radomly divided into control,ISO,KXA low-dose and high-dose groups according to the randomized block design method,and were administered by intragastric administration for 10 consecutive days,and on the 9th and 10th days,rats were injected with ISO for 2 consecutive days to construct an acute myocardial ischemia model to evaluate the improvement of myocardial ischemia by KXA.In addition,the diastolic effect of KXA on rat thoracic aorta and its regulation of ion channels were tested by in vitro vascular tension test.The influence of KXA on the expression of calcium-CaM-dependent protein kinase Ⅱ(CaMK Ⅱ)/extracellular regulated protein kinases(ERK)signaling pathway has also been tested.Results: KXA significantly reduced the ISO-induced increase in ST-segment,interventricular septal thickness,cardiac mass index and cardiac tissue pathological changes in rats.Moreover,the relaxation of isolated thoracic arterial rings that had been precontracted using norepinephrine(NE)or potassium chloride(KCl)was increased after KXA treatment in an endothelium-independent manner,and was attenuated by preincubation with verapamil,but not with tetraethylammonium chloride,4-aminopyridine,glibenclamide,or barium chloride.KXA pretreatment attenuated vasoconstriction induced by CaCl_(2)in Ca^(2+)-free solutions containing K^(+) or NE.In addition,KXA pretreatment inhibited accumulation of Ca^(2+)in A7r5 cells mediated by KCl and NE and significantly decreased p-CaMK Ⅱ and p-ERK levels.Conclusion: KXA may inhibit influx and release of calcium and activate the CaMK Ⅱ/ERK signaling pathway to produce vasodilatory effects,thereby improving myocardial injury.展开更多
文摘利用平板对峙实验及小区试验,筛选高效防治西洋参立枯病的木霉菌株,并采用ITS(internal transcribed spacer)序列分析与形态学特征相结合的方法进行种类鉴定。结果表明,供试木霉菌株在PDA平板上对立枯丝核菌( R. solani )均有抑制作用,菌株HB20111效果最好,抑制率达99.33%;菌株HB20111对西洋参立枯病的小区效果显著,与其他处理间存在显著性差异( P <0.05);第1年拌种处理对西洋参立枯病的防治效果、单株参增重率及总参增重率分别达71.81%、 33.54%和92.75%;第2年蘸根处理对西洋参立枯病的防治效果、单株参增重率及总参增重率分别达91.04%、158.06%和1 369.34%;菌株HB20111的分生孢子梗为典型的单侧分枝,其他形态学特征同深绿木霉( T. atroviride )一致,ITS序列与 T. atroviride 同源性达 100.0%,将菌株HB20111鉴定为 T. atroviride 。HB20111生长速度快,产孢能力强,生防效果高,有望替代化学杀菌剂应用于西洋参种植中。
基金Supported by Natural Science Foundation of Fujian Province(No.2018J01884)Science and Technology Major Project of Fujian Province(No.2019YZ014004)+1 种基金Fujian Provincial Health and Family Planning Commission(No.2018-CX-42)the National Natural Science Foundation of China(No.81774135)。
文摘Objective: To explore the effect of Kuanxiong Aerosol(KXA)on isoproterenol(ISO)-induced myocardial injury in rat models.Methods: Totally 24 rats were radomly divided into control,ISO,KXA low-dose and high-dose groups according to the randomized block design method,and were administered by intragastric administration for 10 consecutive days,and on the 9th and 10th days,rats were injected with ISO for 2 consecutive days to construct an acute myocardial ischemia model to evaluate the improvement of myocardial ischemia by KXA.In addition,the diastolic effect of KXA on rat thoracic aorta and its regulation of ion channels were tested by in vitro vascular tension test.The influence of KXA on the expression of calcium-CaM-dependent protein kinase Ⅱ(CaMK Ⅱ)/extracellular regulated protein kinases(ERK)signaling pathway has also been tested.Results: KXA significantly reduced the ISO-induced increase in ST-segment,interventricular septal thickness,cardiac mass index and cardiac tissue pathological changes in rats.Moreover,the relaxation of isolated thoracic arterial rings that had been precontracted using norepinephrine(NE)or potassium chloride(KCl)was increased after KXA treatment in an endothelium-independent manner,and was attenuated by preincubation with verapamil,but not with tetraethylammonium chloride,4-aminopyridine,glibenclamide,or barium chloride.KXA pretreatment attenuated vasoconstriction induced by CaCl_(2)in Ca^(2+)-free solutions containing K^(+) or NE.In addition,KXA pretreatment inhibited accumulation of Ca^(2+)in A7r5 cells mediated by KCl and NE and significantly decreased p-CaMK Ⅱ and p-ERK levels.Conclusion: KXA may inhibit influx and release of calcium and activate the CaMK Ⅱ/ERK signaling pathway to produce vasodilatory effects,thereby improving myocardial injury.
