目的:了解育龄期癌症女性病人生育忧虑发生现状,为相关干预提供依据。方法:检索Web of Science、PubMed、Embase、Cochrane Library、EBSCO、中国知网、维普、万方数据库、中国生物医学文献数据库自建库至2022年4月发表的育龄期癌症女...目的:了解育龄期癌症女性病人生育忧虑发生现状,为相关干预提供依据。方法:检索Web of Science、PubMed、Embase、Cochrane Library、EBSCO、中国知网、维普、万方数据库、中国生物医学文献数据库自建库至2022年4月发表的育龄期癌症女性病人生育忧虑相关文献,采用Stata 16.0软件进行Meta分析。结果:共纳入27篇文献,6315例育龄期癌症女性病人。Meta分析结果显示,育龄期癌症女性病人生育忧虑评分为57.92[95%CI(56.41,59.43)]分,得分较高的两个维度为子女健康10.84[95%CI(10.57,11.11)]分和自身健康10.62[95%CI(10.25,10.98)]分。亚组分析显示,无子女、有生育意愿、文化程度高、月收入低、未婚、仅育女孩、联合采用放化疗、癌症分期Ⅰ期、甲状腺癌、中国病人生育忧虑更重。结论:育龄期癌症女性病人生育忧虑水平偏高,主要忧虑子女与自身健康。婚育、经济文化、疾病治疗、国家等特征不同的病人,生育忧虑水平存在差异。展开更多
The lack of selective extraction specific for lysine-acetylated proteins has been a major problem in the field of acetylation biology,though acetylation plays a key role in many biological processes.In this paper,we r...The lack of selective extraction specific for lysine-acetylated proteins has been a major problem in the field of acetylation biology,though acetylation plays a key role in many biological processes.In this paper,we report for the first time the proteomic screening of lysine-acetylated proteins from a mouse liver tissue,by a new approach of immunoaffinity purification of lysine-acetylated peptides combined with nano-HPLC/MS/MS analysis.We have found 20 lysine-acetylated proteins with 21 lysine-acetylated sites,among which 12 lysine-acetylated proteins and 16 lysine-acetylated sites have never been reported before.Notably,three acetyltransferases harboring in mitochondrion are newly discovered acetyltransferases responsible for the acetylation of nonhistone proteins.We have explored the significant patterns of residue preference by the hierarchical clustering analysis of amino acid residues surrounding acetylation sites,which could be helpful to the prediction of new sites of lysine acetylation.Our findings provide more candidates for studying the important roles played by acetylation in diverse cellular pathways and related human diseases.展开更多
文摘目的:了解育龄期癌症女性病人生育忧虑发生现状,为相关干预提供依据。方法:检索Web of Science、PubMed、Embase、Cochrane Library、EBSCO、中国知网、维普、万方数据库、中国生物医学文献数据库自建库至2022年4月发表的育龄期癌症女性病人生育忧虑相关文献,采用Stata 16.0软件进行Meta分析。结果:共纳入27篇文献,6315例育龄期癌症女性病人。Meta分析结果显示,育龄期癌症女性病人生育忧虑评分为57.92[95%CI(56.41,59.43)]分,得分较高的两个维度为子女健康10.84[95%CI(10.57,11.11)]分和自身健康10.62[95%CI(10.25,10.98)]分。亚组分析显示,无子女、有生育意愿、文化程度高、月收入低、未婚、仅育女孩、联合采用放化疗、癌症分期Ⅰ期、甲状腺癌、中国病人生育忧虑更重。结论:育龄期癌症女性病人生育忧虑水平偏高,主要忧虑子女与自身健康。婚育、经济文化、疾病治疗、国家等特征不同的病人,生育忧虑水平存在差异。
基金support from the National Natural Science Foundation of China(Grant Nos.20675020&20735005)the National Science Foundation for Post-doctor of China(Grant No.20080440075)+2 种基金the Shanghai Leading Academic Discipline Project(Grant No.B109)the Shanghai Municipal Health Bureau(Grant No.2008Y024)the Shanghai Postdoctoral Sustentation Fund
文摘The lack of selective extraction specific for lysine-acetylated proteins has been a major problem in the field of acetylation biology,though acetylation plays a key role in many biological processes.In this paper,we report for the first time the proteomic screening of lysine-acetylated proteins from a mouse liver tissue,by a new approach of immunoaffinity purification of lysine-acetylated peptides combined with nano-HPLC/MS/MS analysis.We have found 20 lysine-acetylated proteins with 21 lysine-acetylated sites,among which 12 lysine-acetylated proteins and 16 lysine-acetylated sites have never been reported before.Notably,three acetyltransferases harboring in mitochondrion are newly discovered acetyltransferases responsible for the acetylation of nonhistone proteins.We have explored the significant patterns of residue preference by the hierarchical clustering analysis of amino acid residues surrounding acetylation sites,which could be helpful to the prediction of new sites of lysine acetylation.Our findings provide more candidates for studying the important roles played by acetylation in diverse cellular pathways and related human diseases.
