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基于两样本孟德尔随机化的摄入咖啡与前列腺癌因果关系研究
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作者 陈劲果 王之仕 黄卫 《肿瘤防治研究》 CAS 2024年第1期49-54,共6页
目的采用两样本孟德尔随机化方法探讨摄入咖啡与前列腺癌之间是否存在因果关系。方法摄入咖啡(暴露)与前列腺癌(结局)的全基因组关联研究数据来自英国生物样本库中两个独立的数据集,利用逆方差加权法、加权中位数法以及MR-Egger法进行... 目的采用两样本孟德尔随机化方法探讨摄入咖啡与前列腺癌之间是否存在因果关系。方法摄入咖啡(暴露)与前列腺癌(结局)的全基因组关联研究数据来自英国生物样本库中两个独立的数据集,利用逆方差加权法、加权中位数法以及MR-Egger法进行两样本孟德尔随机化研究,以OR值及95%CI代表摄入咖啡与前列腺癌的关联。此外,采用MR-Egger法进行多效性检验及异质性检验,留一法进行敏感性分析。结果总共筛选38个SNP作为工具变量,逆方差加权法显示摄入咖啡可能降低前列腺癌风险(OR=0.994,95%CI:0.990~0.999,P=0.009),加权中位数法得到的结论一致(OR=0.991,95%CI:0.985~0.999,P=0.018),不过MR-Egger回归未发现摄入咖啡与前列腺癌存在因果关系(OR=0.992,95%CI:0.983~1.000,P=0.084)。MR-Egger法显示工具变量不存在多效性(截距=4.2E-5,P=0.581)及异质性(Q=27.20,P=0.854),敏感性分析显示结论稳健。结论孟德尔随机化分析结果表明摄入咖啡可能减少前列腺癌发病风险。 展开更多
关键词 孟德尔随机化 咖啡 前列腺癌
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基于网络药理学和分子对接研究桃核承气汤治疗慢性前列腺炎的作用机制
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作者 陈劲果 王定国 +2 位作者 邢益涛 王之仕 黄卫 《中国性科学》 2024年第5期122-126,共5页
目的采用网络药理学和分子对接方法研究桃核承气汤治疗慢性前列腺炎的分子机制。方法利用中药系统药理学数据库与分析平台TCMSP及文献筛选桃核承气汤有效活性成分及作用靶点,并采用UniProt数据库将蛋白靶点转换为基因名称。从GeneCard和... 目的采用网络药理学和分子对接方法研究桃核承气汤治疗慢性前列腺炎的分子机制。方法利用中药系统药理学数据库与分析平台TCMSP及文献筛选桃核承气汤有效活性成分及作用靶点,并采用UniProt数据库将蛋白靶点转换为基因名称。从GeneCard和CTD数据库获取慢性前列腺炎的相关疾病靶点,与桃核承气汤作用靶点取交集,获得交集基因。将交集基因提交至STRING数据库获得蛋白互作关系,利用Cytoscape软件绘制蛋白互作网络并采用CytoNCA插件筛选核心蛋白。采用R软件进行KEGG信号通路富集分析。借助PubChem数据库、Chem3D软件、PDB数据库、PyMOL软件、AutoDockTools软件及Vina软件对活性成分与关键靶点进行分子对接。结果桃核承气汤共有136种有效活性成分,作用靶点与疾病靶点有154个交集靶点,去除不含交集靶点的活性成分,最终筛选得到120种活性成分。蛋白互作分析得到蛋白激酶B(AKT)1、丝裂原活化蛋白激酶(MAPK)1、信号传导和转录激活因子3(STAT3)、MAPK3等15种核心蛋白,通路富集分析得到肿瘤坏死因子(TNF)、MAPK、酪氨酸激酶-信号转导和转录激活因子(JAK-STAT)、磷酯酰肌醇3激酶(PI3K)-AKT、核因子-κB(NF-κB)等31条与慢性前列腺炎相关的通路。分子对接显示活性成分与关键靶点具有良好的结合能力。结论桃核承气汤治疗慢性前列腺炎涉及多种中药活性成分、多个作用靶点及多条信号通路。 展开更多
关键词 桃核承气汤 慢性前列腺炎 网络药理学 分子对接 作用机制
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补中益气汤加减治疗前列腺切除术后压力性尿失禁的临床观察
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作者 黄辉虎 黄卫 +4 位作者 王仕钦 彭玉平 朱礼乐 王之仕 邢益涛 《河北中医》 2023年第4期589-592,596,共5页
目的观察补中益气汤加减治疗前列腺切除术后压力性尿失禁的临床效果。方法将84例前列腺切除术后压力性尿失禁患者按照随机数字表法分为2组,对照组42例予西医常规治疗,治疗组42例在对照组基础上联合补中益气汤加减治疗。2组均治疗1个月... 目的观察补中益气汤加减治疗前列腺切除术后压力性尿失禁的临床效果。方法将84例前列腺切除术后压力性尿失禁患者按照随机数字表法分为2组,对照组42例予西医常规治疗,治疗组42例在对照组基础上联合补中益气汤加减治疗。2组均治疗1个月后统计疗效,比较2组治疗前后国际尿失禁咨询委员会尿失禁问卷简表(ICI-Q-SF)(包括漏尿频率、漏尿量及漏尿对生活影响)评分、中医证候(包括夜尿次数、残余尿量、下肢乏力、疲惫、腰膝腿软、畏寒、舌苔及脉象)评分、1 h尿垫试验漏尿量及尿动力学指标[包括最大尿流速(Qmax)、残余尿量(PRV)、最大逼尿肌压(Pdet)及最大膀胱容量(MCC)]变化情况,比较2组治疗期间不良反应发生情况。结果治疗组总有效率95.2%(40/42),不良反应总发生率7.1%(3/42),对照组总有效率76.2%(32/42),不良反应总发生率4.8%(2/42),2组不良反应总发生率比较差异无统计学意义(P>0.05),但治疗组总有效率高于对照组(P<0.05)。与本组治疗前比较,2组治疗后ICI-Q-SF漏尿频率、漏尿量、漏尿对生活影响评分及总分均降低(P<0.05),且治疗组治疗后ICI-Q-SF漏尿频率、漏尿量、漏尿对生活影响评分及总分均低于对照组(P<0.05)。与本组治疗前比较,2组治疗后Qmax、Pdet及MCC均升高(P<0.05),PRV均降低(P<0.05),且治疗组治疗后Qmax、Pdet及MCC均高于对照组(P<0.05),PRV低于对照组(P<0.05)。与本组治疗前比较,2组治疗后中医证候夜尿次数、残余尿量、下肢乏力、疲惫、腰膝腿软、畏寒、舌苔、脉象评分均降低(P<0.05),且治疗组治疗后中医证候各项评分均低于对照组(P<0.05)。与本组治疗前比较,2组治疗后1 h尿垫试验漏尿量均减少(P<0.05),且治疗组治疗后1 h尿垫试验漏尿量少于对照组(P<0.05)。结论补中益气汤加减治疗前列腺切除术后压力性尿失禁临床疗效确切,可有效改善患者尿失禁症状,提高控尿、排尿能力,改善中医证候表现,且安全可靠。 展开更多
关键词 前列腺切除术 尿失禁 手术并发症 中药疗法 补中益气汤
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经前腹腔镜与经后腹腔镜手术在萎缩无功能肾切除中的应用比较 被引量:4
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作者 黄辉虎 黄卫 +3 位作者 王仕钦 彭玉平 王之仕 朱礼乐 《实用医学杂志》 CAS 北大核心 2018年第23期3957-3960,共4页
目的探究经后腹腔镜和经前腹腔镜肾切除术治疗萎缩无功能肾的疗效及其对患者康复的影响。方法选择80例萎缩无功能肾进行肾切除患者为研究对象,选择经后腹腔镜手术患者有40例(后腹腔镜组),选择经前腹腔镜肾切除术的患者有40例(前腹腔镜... 目的探究经后腹腔镜和经前腹腔镜肾切除术治疗萎缩无功能肾的疗效及其对患者康复的影响。方法选择80例萎缩无功能肾进行肾切除患者为研究对象,选择经后腹腔镜手术患者有40例(后腹腔镜组),选择经前腹腔镜肾切除术的患者有40例(前腹腔镜组),比较两组患者手术时间和术前术后的情况,比较两组患者术中和术后的情况,并检测两组患者术前1 d和术后1周血清白蛋白、血红蛋白水平。结果后腹腔镜组术中出血量、术后至开始进食时间、术后肠道恢复时间、术后引流时间、术后输血例数、术后止痛药使用量、术后住院天数和住院费用及并发症发生率均低于前腹腔镜组,差异均具有统计学意义(P <0.05)。术前1 d,两组血清白蛋白、血红蛋白的水平差异均无统计学意义(P> 0.05),后腹腔镜组和前腹腔镜组术后1周,两组血清白蛋白、血红蛋白的水平均降低(P <0.05),而后腹腔镜组血清白蛋白、血红蛋白降低的幅度小于前腹腔镜组(P <0.05)。结论萎缩无功能肾的切除术在疗效上经后腹腔镜入路优于经前腹腔镜入路,经后腹腔镜入路具有术后康复快和创伤小等优点。 展开更多
关键词 萎缩无功能肾 肾切除术 前腹腔镜 后腹腔镜
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miR-223通过调控Keap1/Nrf2/ARE信号通路对前列腺癌细胞损伤的影响
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作者 王之仕 李桂凌 +1 位作者 陈劲果 王宏 《实用医学杂志》 CAS 2024年第17期2375-2380,共6页
目的探究微小RNA-223(miR-223)通过调控Kelch样环氧氯丙烷相关蛋白1(Keap1)/核因子E2相关因子2(Nrf2)/抗氧化反应元件(ARE)信号通路对前列腺癌细胞损伤的影响。方法培养前列腺癌细胞株PC3,且随机将其分为对照组、下调miR-223组、上调miR... 目的探究微小RNA-223(miR-223)通过调控Kelch样环氧氯丙烷相关蛋白1(Keap1)/核因子E2相关因子2(Nrf2)/抗氧化反应元件(ARE)信号通路对前列腺癌细胞损伤的影响。方法培养前列腺癌细胞株PC3,且随机将其分为对照组、下调miR-223组、上调miR-223组。探究miR-223表达、细胞增殖率、细胞迁移数、细胞侵袭数、凋亡率、Keap1/Nrf2/ARE信号通路表达量的变化。结果与对照组比较,下调miR-223组的细胞侵袭数、细胞迁移数、24、48、72 h细胞增殖率、Nrf2、ARE表达上升,miR-223、Keap1、凋亡率表达降低(P<0.05);与下调miR-223组比较,上调miR-223组的24、48、72 h细胞增殖率、细胞侵袭数、细胞迁移数、ARE、Nrf2表达下降,miR-223、凋亡率、Keap1表达升高(P<0.05)。结论调节miR-223可有效改善前列腺细胞损伤,其机制可能与Keap1/Nrf2/ARE信号通路有关。 展开更多
关键词 前列腺癌 微小RNA-223 Kelch样环氧氯丙烷相关蛋白1/核因子E2相关因子2/抗氧化反应元件 细胞损伤
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Study on the dynamic characteristics of flow over building cluster at high Reynolds number by large eddy simulation 被引量:5
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作者 wang BoBin wang zhishi +1 位作者 CUI GuiXiang ZHANG ZhaoShun 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2014年第6期1144-1159,共16页
In this paper,the dynamic characteristics of building clusters are simulated by large eddy simulation at high Reynolds number for both homogeneous and heterogeneous building clusters.To save the computational cost a c... In this paper,the dynamic characteristics of building clusters are simulated by large eddy simulation at high Reynolds number for both homogeneous and heterogeneous building clusters.To save the computational cost a channel-like flow model is applied to the urban canopy with free slip condition at the upper boundary.The results show that the domain height is an important parameter for correct evaluation of the dynamic characteristics.The domain height must be greater than 8h(h is the average building height)in order to obtain correct roughness height while displacement height and roughness sublayer are less sensitive to the domain height.The Reynolds number effects on the dynamic characteristics and flow patterns are investigated.The turbulence intensity is stronger inside building cluster at high Reynolds number while turbulence intensity is almost unchanged with Reynolds number above the building cluster.Roughness height increases monotonously with Reynolds number by 20%from Re*=103 to Re*=105 but displacement height is almost unchanged.Within the canopy layer of heterogeneous building clusters,flow structures vary between buildings and turbulence is more active at high Reynolds number. 展开更多
关键词 高雷诺数 动态特性 大涡模拟 建筑群 建筑物高度 湍流强度 雷诺数效应 粗糙度
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A composite model for complex building street configuration in a large eddy simulation of local urban atmospheric environment 被引量:3
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作者 LIU YuShi CUI GuiXiang +3 位作者 wang zhishi HUANG WeiXi XU ChunXiao ZHANG ZhaoShun 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2011年第4期716-723,共8页
The wind field and pollutant dispersion are predicted numerically in a local urban area with crowded buildings and heavy traffic.A domain decomposition method is used in the large eddy simulation,in which the urban ar... The wind field and pollutant dispersion are predicted numerically in a local urban area with crowded buildings and heavy traffic.A domain decomposition method is used in the large eddy simulation,in which the urban area is decomposed into a central area,where pollution is the major concern,and a surrounding region,where the pollutant distribution is not important.The composite model is proposed for the complex building-street layout.The fine grid mesh is used to resolve the buildings in the central area while the buildings are treated as roughness elements in the surrounding region where the coarse grids are used.The proposed numerical method is applied to simulate the wind field and pollutant dispersion from vehicle exhaust in the Rua Do Campo area of Macao.The results show that the composite model is an appropriate method for predicting wind field and pollutant dispersion in the crowded building area. 展开更多
关键词 大涡模拟 组合模型 城市大气环境 污染物扩散 配置 域分解法 建筑面积 污染物分布
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Large eddy simulation of city micro-atmospheric environment 被引量:2
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作者 CUI GuiXiang SHI RuiFeng +2 位作者 wang zhishi XU ChunXiao ZHANG ZhaoShun 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2008年第8期933-944,共12页
Air quality is one of the important conditions for a better residence life in the populated urban area and it is closed related to the micro-atmospheric environment. Atmospheric environment is controlled by air motion... Air quality is one of the important conditions for a better residence life in the populated urban area and it is closed related to the micro-atmospheric environment. Atmospheric environment is controlled by air motion with multi-scales in the city,while air flows in the residence area are of micro-scale atmospheric motion. This paper introduces a modern numerical simulation method,i.e. large eddy simulation(LES) ,for studying micro-atmospheric flows in the city residence area. For the complex flow features in the residence area,the proper application of LES is studied and various numerical methods are compared in order to investigate their effects on the prediction accuracy of micro-atmospheric flows,for instance,roughness elements and immersed boundary method for complex terrain,different subgrid models and so on. The wind field(including turbulence properties) and contaminant dispersion are computed by the proposed method for a model and a realistic residence area,and the numerical results are in good agreement with the experimental measurements. 展开更多
关键词 micro-atmospheric environment TURBULENCE DISPERSION large EDDY simulation
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A toxicological study of inhalable particulates by plasmid DNA assay:A case study from Macao 被引量:1
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作者 SHAO LongYi SHEN RongRong +3 位作者 wang Jing wang zhishi TANG Uwa YANG ShuShen 《Science China Earth Sciences》 SCIE EI CAS 2013年第6期1037-1043,共7页
Oxidative damage to plasmid DNA induced by airborne PM 10(particulate matter with an aerodynamic diameter of 10 μm or less) is caused by the bioavailable(i.e.,soluble) heavy metals on the particle surface.However,qua... Oxidative damage to plasmid DNA induced by airborne PM 10(particulate matter with an aerodynamic diameter of 10 μm or less) is caused by the bioavailable(i.e.,soluble) heavy metals on the particle surface.However,quantitative analyses of the links between PM 10 and oxidative damage are limited.In this study,plasmid DNA assay and ICP-MS were applied to study oxidative capacity and trace element compositions,respectively,of summer and winter PM 10 samples collected at several sites(Sun Yat Sen Municipal Park(SYSP) and Av.de Horta e Costa(AHC) on the Macao peninsula and Macao University on Taipa Island(TI)) in Macao.At AHC and TI,the oxidative capacity of PM 10 collected in winter was higher than that collected in summer,for both the whole sample and the water-soluble fraction.In contrast,no seasonal variation was noted at SYSP.PM 10 exhibited the highest oxidative capacity at SYSP and lowest oxidative capacity at TI in both seasons,demonstrating that the PM 10 collected on the Macao peninsula had a higher toxicity than that from Taipa Island.ICP-MS analyses revealed that the concentrations of total analyzed trace elements and their water-soluble components in PM 10 from TI and AHC were higher in winter than in summer,whereas SYSP displayed the opposite trend.The extents of oxidative damage induced by the water-soluble fractions and intact whole particles were generally similar,implying that the oxidative damage caused by particles in Macao resulted mainly from the water-soluble fraction.The oxidative capacities of PM 10 were positively correlated with both whole and soluble Zn at the 95% confidence level,indicating that Zn was the major element responsible for the oxidative damage caused by particles in Macao.Other heavy metals,such as Cr,Cu,Cd,Ni,As,and Pb,also exhibited elevated concentrations,and the potential health impacts of these metals should be considered. 展开更多
关键词 可吸入颗粒物 质粒DNA DNA检测 澳门 毒理学 微量元素组成 ICP-MS 水溶性成分
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