目的探讨血浆动脉粥样硬化指数(AIP)与妊娠期糖尿病(GDM)及妊娠结局的关系。方法选取2020年10月—2021年5月于安徽医科大学第一附属医院内分泌科行75 g口服葡萄糖耐量试验的妊娠24~28周的435例孕妇,分为GDM组(217例)和正常对照组(NGT组,...目的探讨血浆动脉粥样硬化指数(AIP)与妊娠期糖尿病(GDM)及妊娠结局的关系。方法选取2020年10月—2021年5月于安徽医科大学第一附属医院内分泌科行75 g口服葡萄糖耐量试验的妊娠24~28周的435例孕妇,分为GDM组(217例)和正常对照组(NGT组,218例)。记录所有研究对象临床资料,比较两组临床指标和AIP,采用Pearson相关性分析和多因素logistic回归分析进一步分析GDM患者妊娠中期AIP水平与不良妊娠结局的关系。结果与NGT组比较,GDM组年龄、妊娠前体质量、妊娠前体质量指数(BMI)、空腹血糖(FBG)、1 h血糖(1 h BG)、2 h血糖(2 h BG)、空腹胰岛素(FINS)、2 h胰岛素(2 h INS)、空腹C肽(FCP)、1 h C肽(1 h CP)、2 h C肽(2 h CP)、胰岛素抵抗指数(HOMA-IR)、血糖曲线下面积(AUC-G)、胰岛素曲线下面积(AUC-I)、三酰甘油(TG)、AIP升高,胰岛β细胞功能指数(HOMA-β)、胰岛素敏感指数(ISI)、高密度脂蛋白胆固醇(HDL-C)降低,差异均有统计学意义(均P<0.05)。Pearson相关分析结果显示:GDM组AIP与孕周、妊娠前体质量、妊娠前BMI、FBG、2 h BG、FINS、1 h INS、2 h INS、FCP、1 h CP、2 h CP、HOMA-IR、HOMA-β、AUC-G、AUC-I、TG均呈正相关(r=0.141,0.213,0.222,0.221,0.229,0.324,0.136,0.185,0.262,0.135,0.227,0.341,0.172,0.180,0.165,0.886,均P<0.05),与ISI、TC、HDL-C、LDL-C均呈负相关(r=-0.341,-0.212,-0.709,-0.346,均P<0.05)。NGT组中,40例发生不良妊娠结局,发生率为18.35%;GDM组中,45例发生不良妊娠结局,发生率为20.74%。多因素logistic回归分析结果显示:AIP、TG是GDM患者不良妊娠结局的独立危险因素(OR=1.016,2.679,均P<0.05)。结论GDM患者AIP水平较正常糖耐量孕妇升高,且与糖脂代谢、胰岛素抵抗密切相关。GDM患者的不良妊娠结局发生率较高,AIP、TG是GDM患者不良妊娠结局的独立危险因素,可考虑针对上述患者采取相关干预措施,以改善患者的妊娠结局。展开更多
目的观察医用臭氧水对兔膝关节骨性关节炎关节软骨基质金属蛋白酶1/3/13(MMP-1/3/13)表达的影响。方法成年新西兰大白兔36只,随机分为对照组12只、骨性关节炎组12只与医用臭氧水治疗组12只。采用Hulth法将骨性关节炎组与医用臭氧水治疗...目的观察医用臭氧水对兔膝关节骨性关节炎关节软骨基质金属蛋白酶1/3/13(MMP-1/3/13)表达的影响。方法成年新西兰大白兔36只,随机分为对照组12只、骨性关节炎组12只与医用臭氧水治疗组12只。采用Hulth法将骨性关节炎组与医用臭氧水治疗组实验动物制成膝关节骨性关节炎模型。待造模成功后,医用臭氧水治疗组向兔膝关节腔内注射医用臭氧水2ml,注射浓度为20μg/ml,一周一次,共治疗3次,骨性关节炎组兔关节腔内注射空气;对照组实验动物不作处理。在造模成功时和处死前测定兔膝关节活动度,末次干预1周后,用空气栓塞法处死实验兔,取下实验兔右膝关节软骨进行组织匀浆,采用Western blot与real time PCR方法测定关节软骨中MMP-1、MMP-3与MMP-13的含量变化。结果与对照组相比,造模成功后的骨性关节炎组和医用臭氧水治疗组兔膝关节活动度显著降低,P<0.01;与骨性关节炎组相比,采用医用臭氧水治疗后,医用臭氧水治疗组兔膝关节活动度显著升高,P<0.01。骨性关节炎组大鼠膝关节软骨组织中MMP-1、MMP-3与MMP-13的蛋白与m RNA表达水平比对照组明显升高,而医用臭氧水治疗组比骨性关节炎组显著降低,P<0.01。结论医用臭氧水对骨性关节炎的治疗作用可能与降低早期骨性关节炎关节软骨中MMP-1、MMP-3与MMP-13的表达水平相关。展开更多
Background Efficient cell adhesion and proliferation is a central issue in cell-based tissue engineering, which offers great promise for repair of urethral defects or strictures. This study evaluated the adhesion and ...Background Efficient cell adhesion and proliferation is a central issue in cell-based tissue engineering, which offers great promise for repair of urethral defects or strictures. This study evaluated the adhesion and growth of rabbit uroepithelium on a surface-modified three-dimensional poly-L-lactic acid （PLLA） scaffold.Methods Urethral mucosa were harvested from male New Zealand rabbits and the urothelium were dissociated and then cultured. Immunocytochemistry on cultured uroepithelium for pancytokeratin and uroplakin Ⅱ and TE-7 confirmed pure populations. After in vitro proliferation, cells were seeded onto a surface-modified urethral scaffold with non-knitted filaments. The morphology and viability of the cells were examined by immunohistochemical and fluorescence staining.Inverted and scanning microscopes were used to document cell growth and adhesion.Results Three to five days after primary culture, the uroepithelial cells gradually became confluent, assuming a cobblestone pattern. The filaments of the urethral scaffold had excellent biocompatibility and allowed growth of the uroepithelium, without affecting viability. The uroepithelial cells adhered to and grew well on the scaffold. After 3-7 days,the cells grew vigorously and meshes of the scaffold were full of uroepitheliums.Conclusions The surface-modified urethral scaffold with non-knitted filaments allows the growth of uroepithelium and can serve as a carrier for the tissue engineering of urethra.展开更多
文摘目的探讨血浆动脉粥样硬化指数(AIP)与妊娠期糖尿病(GDM)及妊娠结局的关系。方法选取2020年10月—2021年5月于安徽医科大学第一附属医院内分泌科行75 g口服葡萄糖耐量试验的妊娠24~28周的435例孕妇,分为GDM组(217例)和正常对照组(NGT组,218例)。记录所有研究对象临床资料,比较两组临床指标和AIP,采用Pearson相关性分析和多因素logistic回归分析进一步分析GDM患者妊娠中期AIP水平与不良妊娠结局的关系。结果与NGT组比较,GDM组年龄、妊娠前体质量、妊娠前体质量指数(BMI)、空腹血糖(FBG)、1 h血糖(1 h BG)、2 h血糖(2 h BG)、空腹胰岛素(FINS)、2 h胰岛素(2 h INS)、空腹C肽(FCP)、1 h C肽(1 h CP)、2 h C肽(2 h CP)、胰岛素抵抗指数(HOMA-IR)、血糖曲线下面积(AUC-G)、胰岛素曲线下面积(AUC-I)、三酰甘油(TG)、AIP升高,胰岛β细胞功能指数(HOMA-β)、胰岛素敏感指数(ISI)、高密度脂蛋白胆固醇(HDL-C)降低,差异均有统计学意义(均P<0.05)。Pearson相关分析结果显示:GDM组AIP与孕周、妊娠前体质量、妊娠前BMI、FBG、2 h BG、FINS、1 h INS、2 h INS、FCP、1 h CP、2 h CP、HOMA-IR、HOMA-β、AUC-G、AUC-I、TG均呈正相关(r=0.141,0.213,0.222,0.221,0.229,0.324,0.136,0.185,0.262,0.135,0.227,0.341,0.172,0.180,0.165,0.886,均P<0.05),与ISI、TC、HDL-C、LDL-C均呈负相关(r=-0.341,-0.212,-0.709,-0.346,均P<0.05)。NGT组中,40例发生不良妊娠结局,发生率为18.35%;GDM组中,45例发生不良妊娠结局,发生率为20.74%。多因素logistic回归分析结果显示:AIP、TG是GDM患者不良妊娠结局的独立危险因素(OR=1.016,2.679,均P<0.05)。结论GDM患者AIP水平较正常糖耐量孕妇升高,且与糖脂代谢、胰岛素抵抗密切相关。GDM患者的不良妊娠结局发生率较高,AIP、TG是GDM患者不良妊娠结局的独立危险因素,可考虑针对上述患者采取相关干预措施,以改善患者的妊娠结局。
文摘目的观察医用臭氧水对兔膝关节骨性关节炎关节软骨基质金属蛋白酶1/3/13(MMP-1/3/13)表达的影响。方法成年新西兰大白兔36只,随机分为对照组12只、骨性关节炎组12只与医用臭氧水治疗组12只。采用Hulth法将骨性关节炎组与医用臭氧水治疗组实验动物制成膝关节骨性关节炎模型。待造模成功后,医用臭氧水治疗组向兔膝关节腔内注射医用臭氧水2ml,注射浓度为20μg/ml,一周一次,共治疗3次,骨性关节炎组兔关节腔内注射空气;对照组实验动物不作处理。在造模成功时和处死前测定兔膝关节活动度,末次干预1周后,用空气栓塞法处死实验兔,取下实验兔右膝关节软骨进行组织匀浆,采用Western blot与real time PCR方法测定关节软骨中MMP-1、MMP-3与MMP-13的含量变化。结果与对照组相比,造模成功后的骨性关节炎组和医用臭氧水治疗组兔膝关节活动度显著降低,P<0.01;与骨性关节炎组相比,采用医用臭氧水治疗后,医用臭氧水治疗组兔膝关节活动度显著升高,P<0.01。骨性关节炎组大鼠膝关节软骨组织中MMP-1、MMP-3与MMP-13的蛋白与m RNA表达水平比对照组明显升高,而医用臭氧水治疗组比骨性关节炎组显著降低,P<0.01。结论医用臭氧水对骨性关节炎的治疗作用可能与降低早期骨性关节炎关节软骨中MMP-1、MMP-3与MMP-13的表达水平相关。
基金This work was supported by the grants from the National Natural Science Foundation of China （No. 81070555） and the Major Program of Clinical High and New Technology Research of Hospital.
文摘Background Efficient cell adhesion and proliferation is a central issue in cell-based tissue engineering, which offers great promise for repair of urethral defects or strictures. This study evaluated the adhesion and growth of rabbit uroepithelium on a surface-modified three-dimensional poly-L-lactic acid （PLLA） scaffold.Methods Urethral mucosa were harvested from male New Zealand rabbits and the urothelium were dissociated and then cultured. Immunocytochemistry on cultured uroepithelium for pancytokeratin and uroplakin Ⅱ and TE-7 confirmed pure populations. After in vitro proliferation, cells were seeded onto a surface-modified urethral scaffold with non-knitted filaments. The morphology and viability of the cells were examined by immunohistochemical and fluorescence staining.Inverted and scanning microscopes were used to document cell growth and adhesion.Results Three to five days after primary culture, the uroepithelial cells gradually became confluent, assuming a cobblestone pattern. The filaments of the urethral scaffold had excellent biocompatibility and allowed growth of the uroepithelium, without affecting viability. The uroepithelial cells adhered to and grew well on the scaffold. After 3-7 days,the cells grew vigorously and meshes of the scaffold were full of uroepitheliums.Conclusions The surface-modified urethral scaffold with non-knitted filaments allows the growth of uroepithelium and can serve as a carrier for the tissue engineering of urethra.
