The successful implementation of mass customization lies on reengineeringtechnology and management methods to organize the production. Especially in assembly phase, variousproduct configurations, due-time penalties an...The successful implementation of mass customization lies on reengineeringtechnology and management methods to organize the production. Especially in assembly phase, variousproduct configurations, due-time penalties and order-driven strategy challenge the traditionaloperation and management of assembly lines. The business features and the operation pattern ofassembly line based on mass customization are analyzed. And the research emphatically studiesvarious technologic factors to improve customer satisfaction and their corresponding implementmethods in operating assembly line. In addition, the models are proposed for operating assembly lineunder dynamic process environment in mass customization. A genetic approach is developed to providethe optimal solution to the models. The effectiveness of the proposed approach is evaluated with anindustrial application.展开更多
Nonlinear screening of a test charge in plasma by electrons trapped or untrapped is studied. The obtained results are in rigorous estimations mathematically in comparison with the corresponding Debye screening forms.M...Nonlinear screening of a test charge in plasma by electrons trapped or untrapped is studied. The obtained results are in rigorous estimations mathematically in comparison with the corresponding Debye screening forms.Meanwhile their validity is physically discussed and some confusions in literature are clarified.展开更多
目的比较德谷门冬双胰岛素、门冬胰岛素30在2型糖尿病(T2DM)非肥胖型患者中的血糖控制效果及安全性。方法将T2DM非肥胖型患者依据治疗方式分为试验组和对照组。对照组给予门冬胰岛素30治疗,试验组给予德谷门冬双胰岛素治疗。比较2组治...目的比较德谷门冬双胰岛素、门冬胰岛素30在2型糖尿病(T2DM)非肥胖型患者中的血糖控制效果及安全性。方法将T2DM非肥胖型患者依据治疗方式分为试验组和对照组。对照组给予门冬胰岛素30治疗,试验组给予德谷门冬双胰岛素治疗。比较2组治疗前后胰岛相关指标[空腹C肽(FCP)、餐后2 h C肽(2 h CP)]、血糖控制效果[空腹血糖(FBG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(HbA1c)]、血清25羟基维生素D[25(OH)D]和低血糖发生风险。结果试验组41例,对照组39例。治疗后,试验组和对照组的FCP分别为(0.84±0.09)和(1.07±0.14)nmol·L^(-1),2 h CP分别为(1.03±0.15)和(1.69±0.17)nmol·L^(-1),FBG分别为(5.46±0.57)和(6.18±0.67)mmol·L^(-1),2 h PG分别为(8.17±0.85)和(9.03±0.94)mmol·L^(-1),HbA1c分别为(5.35±0.57)%和(6.47±0.68)%,25(OH)D分别为(26.33±2.75)和(20.54±2.17)nmol·L^(-1),在统计学上差异均有统计学意义(均P<0.05)。治疗后,试验组和对照组的非严重低血糖发生率分别为14.63%和35.90%(P<0.05)。试验组和对照组的严重低血糖发生率分别为9.76%和12.82%,夜间低血糖发生率分别为19.51%和17.95%,在统计学上差异均无统计学意义(均P>0.05)。结论德谷门冬胰岛素方案对T2DM非肥胖型的整体治疗效果优于门冬胰岛素30,前者可有效地调节患者血糖和胰岛细胞功能状态,降低发生非严重低血糖的风险。展开更多
Bovine fetal oviduct epithelial cells were transfected with constructed double marker selective vector(pCE-EGFP-IRES-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant(Neo^r) gen...Bovine fetal oviduct epithelial cells were transfected with constructed double marker selective vector(pCE-EGFP-IRES-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant(Neo^r) genes by electroporation, and a transgenic cell line was obtained. Somatic cell nuclear transfer (SCNT) was cartied out using the transgenic cells as nuclei donor. A total of 424 SCNT embryos were reconstructed and 208 (49.1%) of them developed to blastocyst stage. 17 blastocysts on D 7 after reconstruction were transferred to 17 surrogate calves,and 5 (29.4%) recipients were found to be pregnant. Three of them maintained to term and delivered three cloned calves.PCR and Southern blot analysis confirmed the integration of transgene in all of the three cloned calves. In addition, expression of EGFP was detected in biopsy isolated from the transgenic cloned calves and fibroblasts derived from the biopsy. Our results suggest that transgenic calves could be efficiently produced by SCNT using transgenic cells as nuclei donor. Furthermore, all cloned animals could be ensured to be transgenic by efficiently pre-screening transgenic cells and SCNT embryos using the constructed double marker selective vector.展开更多
基金National Natural Science Foundation of China (No.59889505)
文摘The successful implementation of mass customization lies on reengineeringtechnology and management methods to organize the production. Especially in assembly phase, variousproduct configurations, due-time penalties and order-driven strategy challenge the traditionaloperation and management of assembly lines. The business features and the operation pattern ofassembly line based on mass customization are analyzed. And the research emphatically studiesvarious technologic factors to improve customer satisfaction and their corresponding implementmethods in operating assembly line. In addition, the models are proposed for operating assembly lineunder dynamic process environment in mass customization. A genetic approach is developed to providethe optimal solution to the models. The effectiveness of the proposed approach is evaluated with anindustrial application.
文摘Nonlinear screening of a test charge in plasma by electrons trapped or untrapped is studied. The obtained results are in rigorous estimations mathematically in comparison with the corresponding Debye screening forms.Meanwhile their validity is physically discussed and some confusions in literature are clarified.
文摘目的比较德谷门冬双胰岛素、门冬胰岛素30在2型糖尿病(T2DM)非肥胖型患者中的血糖控制效果及安全性。方法将T2DM非肥胖型患者依据治疗方式分为试验组和对照组。对照组给予门冬胰岛素30治疗,试验组给予德谷门冬双胰岛素治疗。比较2组治疗前后胰岛相关指标[空腹C肽(FCP)、餐后2 h C肽(2 h CP)]、血糖控制效果[空腹血糖(FBG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(HbA1c)]、血清25羟基维生素D[25(OH)D]和低血糖发生风险。结果试验组41例,对照组39例。治疗后,试验组和对照组的FCP分别为(0.84±0.09)和(1.07±0.14)nmol·L^(-1),2 h CP分别为(1.03±0.15)和(1.69±0.17)nmol·L^(-1),FBG分别为(5.46±0.57)和(6.18±0.67)mmol·L^(-1),2 h PG分别为(8.17±0.85)和(9.03±0.94)mmol·L^(-1),HbA1c分别为(5.35±0.57)%和(6.47±0.68)%,25(OH)D分别为(26.33±2.75)和(20.54±2.17)nmol·L^(-1),在统计学上差异均有统计学意义(均P<0.05)。治疗后,试验组和对照组的非严重低血糖发生率分别为14.63%和35.90%(P<0.05)。试验组和对照组的严重低血糖发生率分别为9.76%和12.82%,夜间低血糖发生率分别为19.51%和17.95%,在统计学上差异均无统计学意义(均P>0.05)。结论德谷门冬胰岛素方案对T2DM非肥胖型的整体治疗效果优于门冬胰岛素30,前者可有效地调节患者血糖和胰岛细胞功能状态,降低发生非严重低血糖的风险。
文摘Bovine fetal oviduct epithelial cells were transfected with constructed double marker selective vector(pCE-EGFP-IRES-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant(Neo^r) genes by electroporation, and a transgenic cell line was obtained. Somatic cell nuclear transfer (SCNT) was cartied out using the transgenic cells as nuclei donor. A total of 424 SCNT embryos were reconstructed and 208 (49.1%) of them developed to blastocyst stage. 17 blastocysts on D 7 after reconstruction were transferred to 17 surrogate calves,and 5 (29.4%) recipients were found to be pregnant. Three of them maintained to term and delivered three cloned calves.PCR and Southern blot analysis confirmed the integration of transgene in all of the three cloned calves. In addition, expression of EGFP was detected in biopsy isolated from the transgenic cloned calves and fibroblasts derived from the biopsy. Our results suggest that transgenic calves could be efficiently produced by SCNT using transgenic cells as nuclei donor. Furthermore, all cloned animals could be ensured to be transgenic by efficiently pre-screening transgenic cells and SCNT embryos using the constructed double marker selective vector.
