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Cloning and Sequencing the y Subunit of R-Phycoerythrin from Corallina officinalis
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作者 wangsheng zhongfu-di wuzu-jian linqi-ying xielian-hui 《Acta Botanica Sinica》 CSCD 2004年第10期1135-1140,共6页
The full-length cDNA of the γ subunit of R-phycoerythrin from Corallina officinalis L. was cloned by rapid amplification of cDNA ends (RACE) method, and sequenced. The full-length cDNA is a 2 308 bp consisting of 5&#... The full-length cDNA of the γ subunit of R-phycoerythrin from Corallina officinalis L. was cloned by rapid amplification of cDNA ends (RACE) method, and sequenced. The full-length cDNA is a 2 308 bp consisting of 5' untranslated region (UTR) of 1 203 bp, an open reading frame (ORF) of 960 bp that encodes 320 amino acids, and 3' UTR of 145 bp. The mature γ polypeptide contains two unique internal repeat domains as reported by Apt et al. (2001). Sequence analysis of the different clones revealed different 3'-end sequences at the γ subunit. The difference between the 3'-end sequences suggests that the γ subunit may have more than one copy, or have gone through different post-transcriptional modification. By comparing the DNA and cDNA sequences, we found that the γ subunit is an intronless gene. This is thefirst report of the γ subunit gene of R-phycoerythrin from C. officinalis. 展开更多
关键词 R-藻红蛋白 红藻 蓝藻细菌 克隆 序列分析 珊瑚藻属
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