N-ros is one of the transforming genes in human hepatic cancer cells. It has been found that N-ras was overexpressed at the mENA and protein level in hepatoma cells. In order to explore the biological roles of N-ras i...N-ros is one of the transforming genes in human hepatic cancer cells. It has been found that N-ras was overexpressed at the mENA and protein level in hepatoma cells. In order to explore the biological roles of N-ras in human hepatic oaroinogenesis and the potential application in control of cancer cell growth, a pseudotype retrovirus containing antisense sequence of human N-ras was constructed and packaged. A recombinant rebrovirus vector containing antisense or sense sequences of N-ras oDNA was constructed by pZIP-NeoSV(X)1. The pseudotype virus was packaged and rescued by transfeotion and infection in PA317 and ψ2 helper cells. It has been demonstrated that the pseudotype retrovirus containing antisense N-ras sequence did inhibit the growth of human PLC/PEF/5 hepatoma cells accompanied with inhibition of p21 expression, while the retrovirus containing sense sequence had none. The pseudotype virus had no effect on human diploid fibroblasts.展开更多
In the peralumineous granite of Yajiangqiao zircon population can be divided into two groups (i.e. Zircon Ⅰ and Zircon Ⅱ), which were formed in magmatic chamber and in emplacement place, respectively. The Hf isotope...In the peralumineous granite of Yajiangqiao zircon population can be divided into two groups (i.e. Zircon Ⅰ and Zircon Ⅱ), which were formed in magmatic chamber and in emplacement place, respectively. The Hf isotope compositions of two stages of zircon show that the host magma was essentially derived from crustal material by melting. However, some higher 176Hf/177Hf ratios indicate that the granitic zircons should contain fine crystal of zircon formed in mantle-derived magma. In fact, the backscattered electron imaging and the electron microprobe analysis reveal that there is another type of zircon included within Zircon Ⅰ, which is quite different from Zircon Ⅰ and Zircon Ⅱ in morphology and chemistry. They are considered to be the product of the mantle-derived magma intruded into the granitic magma chamber at the beginning of anatexis. Thus, it is suggested that the formation of Yajiangqiao granite is related to the underplating of mantle magma.展开更多
文摘N-ros is one of the transforming genes in human hepatic cancer cells. It has been found that N-ras was overexpressed at the mENA and protein level in hepatoma cells. In order to explore the biological roles of N-ras in human hepatic oaroinogenesis and the potential application in control of cancer cell growth, a pseudotype retrovirus containing antisense sequence of human N-ras was constructed and packaged. A recombinant rebrovirus vector containing antisense or sense sequences of N-ras oDNA was constructed by pZIP-NeoSV(X)1. The pseudotype virus was packaged and rescued by transfeotion and infection in PA317 and ψ2 helper cells. It has been demonstrated that the pseudotype retrovirus containing antisense N-ras sequence did inhibit the growth of human PLC/PEF/5 hepatoma cells accompanied with inhibition of p21 expression, while the retrovirus containing sense sequence had none. The pseudotype virus had no effect on human diploid fibroblasts.
基金supposed by the National Natural Science Foundation of China(Grant Nos.40172023 and 40132010).
文摘In the peralumineous granite of Yajiangqiao zircon population can be divided into two groups (i.e. Zircon Ⅰ and Zircon Ⅱ), which were formed in magmatic chamber and in emplacement place, respectively. The Hf isotope compositions of two stages of zircon show that the host magma was essentially derived from crustal material by melting. However, some higher 176Hf/177Hf ratios indicate that the granitic zircons should contain fine crystal of zircon formed in mantle-derived magma. In fact, the backscattered electron imaging and the electron microprobe analysis reveal that there is another type of zircon included within Zircon Ⅰ, which is quite different from Zircon Ⅰ and Zircon Ⅱ in morphology and chemistry. They are considered to be the product of the mantle-derived magma intruded into the granitic magma chamber at the beginning of anatexis. Thus, it is suggested that the formation of Yajiangqiao granite is related to the underplating of mantle magma.
