In this study,Ni_(2)P/CdS composites were constructed by depositing non-precious metal co-catalyst Ni_(2)P on a one-dimensional network of CdS using a simple in-situ photodeposition method.The prepared photocatalysts ...In this study,Ni_(2)P/CdS composites were constructed by depositing non-precious metal co-catalyst Ni_(2)P on a one-dimensional network of CdS using a simple in-situ photodeposition method.The prepared photocatalysts promoted the decomposition of ethanol into high-value-added products while generating hydrogen.The composite photoanodes loaded with the Ni_(2)P co-catalysts showed significantly higher ethanol conversion and hydrogen production in the visible light region,which was almost three times higher than that of pure CdS.The main products of photocatalytic ethanol production are acetaldehyde(AA)and 2,3-butanediol(2,3-BDA).Compared with CdS,the selectivity of the composite photocatalysts for converting ethanol to acetaldehyde was significantly improved(62% to 78%).Characterization of the prepared photocatalysts confirmed that the loading of Ni_(2)P co-catalysts on CdS not only broadened the optical region of the catalysts for trapping light but also effectively promoted the separation and transfer of charge carriers,which significantly improved the photocatalytic efficiency of ethanol conversion and hydrogen production in the catalysts.It has been proven through Electron Paramagnetic Resonance testing that loading a Ni_(2)P co-catalyst on CdS is beneficial for the adsorption of hydroxyethyl radicals(*CH(OH)CH_(3)),thereby further improving the selectivity of acetaldehyde.This study plays an important role in the rational design of composite catalyst structures and the introduction of co-catalysts to improve catalyst performance,promote green chemistry,advocate a low-carbon society,and promote sustainable development.展开更多
为了从全基因组和转录组水平鉴定响应盐胁迫的小麦DREB(dehydration responsive element binding,DREB)基因,该研究对小麦耐盐材料CH7034苗期施加盐胁迫后的根部样本进行Illumina转录组测序,从中分离TaDREB家族成员的表达数据和可变剪...为了从全基因组和转录组水平鉴定响应盐胁迫的小麦DREB(dehydration responsive element binding,DREB)基因,该研究对小麦耐盐材料CH7034苗期施加盐胁迫后的根部样本进行Illumina转录组测序,从中分离TaDREB家族成员的表达数据和可变剪接信息,并对其下游靶基因进行预测;利用qRT-PCR对盐胁迫响应TaDREB成员和预测靶基因进行验证。结果显示:(1)从小麦中共鉴定出48个DREB成员(204个拷贝序列),命名为TaDREB1~TaDREB48,分布于21条染色体。(2)TaDREB家族分为14组(G1~G14),位于G2、G5、G10和G14的TaDREB成员受NaCl胁迫后转录水平均无显著变化,其余组中共有25个(52%)TaDREB成员表现出对盐胁迫不同程度的响应;其中有9个成员在盐胁迫后持续上调(含5个新报道基因),有2个成员表现为持续下调;蛋白互作预测结果显示,下调成员TaDREB35的编码蛋白可能会受到1个小麦RING型E3泛素连接酶作用而降解。(3)盐胁迫后有9个成员TaDREB3、TaDREB6、TaDREB16、TaDREB19、TaDREB21、TaDREB24、TaDREB25.12、TaDREB43和TaDREB47发生了可变剪切变化。(4)从转录组差异表达基因中进一步鉴定出3个起始密码子上游2000 bp序列,包含DRE/CRT元件且在A/B/D组间表达趋势一致的候选靶基因TaRD29、TaGLOS和TaCKX。(5)qRT-PCR验证结果显示,上调成员中,除TaDREB19外,其余成员以及TaDREB16均表现出持续上升的趋势;下调成员中只有TaDREB25和TaDREB35的表达量呈持续下降的趋势;3个预测靶基因的表达量均持续上升,验证结果与转录组测序结果一致。该研究鉴定出的11个盐胁迫响应TaDREB成员以及预测的3个下游靶基因为小麦耐盐机制解析和分子育种奠定了基础。展开更多
基金supported by the National Natural Science Foundation of China(22075197,22278290)the Shanxi Provincial Natural Science Foundation of China(202103021224079,201903D421081)the Research and Development Project of Key Core and Common Technology of Shanxi Province(20201102018)。
文摘In this study,Ni_(2)P/CdS composites were constructed by depositing non-precious metal co-catalyst Ni_(2)P on a one-dimensional network of CdS using a simple in-situ photodeposition method.The prepared photocatalysts promoted the decomposition of ethanol into high-value-added products while generating hydrogen.The composite photoanodes loaded with the Ni_(2)P co-catalysts showed significantly higher ethanol conversion and hydrogen production in the visible light region,which was almost three times higher than that of pure CdS.The main products of photocatalytic ethanol production are acetaldehyde(AA)and 2,3-butanediol(2,3-BDA).Compared with CdS,the selectivity of the composite photocatalysts for converting ethanol to acetaldehyde was significantly improved(62% to 78%).Characterization of the prepared photocatalysts confirmed that the loading of Ni_(2)P co-catalysts on CdS not only broadened the optical region of the catalysts for trapping light but also effectively promoted the separation and transfer of charge carriers,which significantly improved the photocatalytic efficiency of ethanol conversion and hydrogen production in the catalysts.It has been proven through Electron Paramagnetic Resonance testing that loading a Ni_(2)P co-catalyst on CdS is beneficial for the adsorption of hydroxyethyl radicals(*CH(OH)CH_(3)),thereby further improving the selectivity of acetaldehyde.This study plays an important role in the rational design of composite catalyst structures and the introduction of co-catalysts to improve catalyst performance,promote green chemistry,advocate a low-carbon society,and promote sustainable development.
文摘为了从全基因组和转录组水平鉴定响应盐胁迫的小麦DREB(dehydration responsive element binding,DREB)基因,该研究对小麦耐盐材料CH7034苗期施加盐胁迫后的根部样本进行Illumina转录组测序,从中分离TaDREB家族成员的表达数据和可变剪接信息,并对其下游靶基因进行预测;利用qRT-PCR对盐胁迫响应TaDREB成员和预测靶基因进行验证。结果显示:(1)从小麦中共鉴定出48个DREB成员(204个拷贝序列),命名为TaDREB1~TaDREB48,分布于21条染色体。(2)TaDREB家族分为14组(G1~G14),位于G2、G5、G10和G14的TaDREB成员受NaCl胁迫后转录水平均无显著变化,其余组中共有25个(52%)TaDREB成员表现出对盐胁迫不同程度的响应;其中有9个成员在盐胁迫后持续上调(含5个新报道基因),有2个成员表现为持续下调;蛋白互作预测结果显示,下调成员TaDREB35的编码蛋白可能会受到1个小麦RING型E3泛素连接酶作用而降解。(3)盐胁迫后有9个成员TaDREB3、TaDREB6、TaDREB16、TaDREB19、TaDREB21、TaDREB24、TaDREB25.12、TaDREB43和TaDREB47发生了可变剪切变化。(4)从转录组差异表达基因中进一步鉴定出3个起始密码子上游2000 bp序列,包含DRE/CRT元件且在A/B/D组间表达趋势一致的候选靶基因TaRD29、TaGLOS和TaCKX。(5)qRT-PCR验证结果显示,上调成员中,除TaDREB19外,其余成员以及TaDREB16均表现出持续上升的趋势;下调成员中只有TaDREB25和TaDREB35的表达量呈持续下降的趋势;3个预测靶基因的表达量均持续上升,验证结果与转录组测序结果一致。该研究鉴定出的11个盐胁迫响应TaDREB成员以及预测的3个下游靶基因为小麦耐盐机制解析和分子育种奠定了基础。
基金Special Talents Science and Technology Innovation Project of Shanxi Province of China(201705D211007)Shanxi Provincial Natural Science Foundation of China(201801D121099,201801D221140,201903D421081)。
