The purpose of the present study was to synthesize a new composites scaffold containing poly(γ-benzyl-L-glutamate) modified hydroxyapatite/(poly(L-lactic acid))(PBLG-g-HA/PLLA) and to investigate their in vit...The purpose of the present study was to synthesize a new composites scaffold containing poly(γ-benzyl-L-glutamate) modified hydroxyapatite/(poly(L-lactic acid))(PBLG-g-HA/PLLA) and to investigate their in vitro behaviour on bone mesenchymal stromal cells(BMSCs). The results demonstrated that BMSC proliferation was signifi cantly increased on PBLG-g-HA/PLLA scaffolds after 3 and 7 days post seeding when compared to PLLA and HA/PLLA scaffolds. The in vitro osteogenic differentiation also favoured the composite PBLG-g-HA/PLLA scaffolds when compared to controls by signifi cantly increasing Runx2, ALP or osteocalcin mRNA expression as assessed by real-time PCR. The results illustrate the potential of PBLG-g-HA/PLLA scaffolds for bone tissue engineering applications. And the in vivo testing further confi rms the PBLG-gHA/PLLA scaffolds' potentioal for healing critical bone defects.展开更多
基金Funded by the National Natural Science Foundation of China(81271108&51203073)the Project from Education Department of Jiangxi Province(GJJ13107)
文摘The purpose of the present study was to synthesize a new composites scaffold containing poly(γ-benzyl-L-glutamate) modified hydroxyapatite/(poly(L-lactic acid))(PBLG-g-HA/PLLA) and to investigate their in vitro behaviour on bone mesenchymal stromal cells(BMSCs). The results demonstrated that BMSC proliferation was signifi cantly increased on PBLG-g-HA/PLLA scaffolds after 3 and 7 days post seeding when compared to PLLA and HA/PLLA scaffolds. The in vitro osteogenic differentiation also favoured the composite PBLG-g-HA/PLLA scaffolds when compared to controls by signifi cantly increasing Runx2, ALP or osteocalcin mRNA expression as assessed by real-time PCR. The results illustrate the potential of PBLG-g-HA/PLLA scaffolds for bone tissue engineering applications. And the in vivo testing further confi rms the PBLG-gHA/PLLA scaffolds' potentioal for healing critical bone defects.