Tumor invasion and metastases is one of the leading causes of tumor-related death while non-small cell lung cancer(NSCLC)is prone to multiple metastases.T umor microenvironment(TEM),especially extracellular matrix(ECM...Tumor invasion and metastases is one of the leading causes of tumor-related death while non-small cell lung cancer(NSCLC)is prone to multiple metastases.T umor microenvironment(TEM),especially extracellular matrix(ECM)and.fibroblasts,plays an important role in tumor metastases and invasion.To better understand the mechanism of NSCLC lung metastases and invasion,we constructed a three-dimensional(3D)co-culture model by seeding A549 tumor cells and human bronchial fibroblasts into decellularized lung scaffolds.Results showed that decellularized mice lung scaffolds retaining intact structure and ECM components,provided a suitable microenvironment for maintaining the viability,morphology of both cells in vitro.3D architecture and pulmonary ECM promoted the mobility/invasion ability of A549 cells by up-regulating the expression of matrix metalloproteinases(MMPs).Meanwhile,factors in TGF-1/Smad4 pathway were significantly up-regulated which may associated with epithelial-mesenchymal transition(EMT)of A549 cells.展开更多
The investigation of syngas flame propagation has great benefits for the effective use of gas turbines.This essay sets out to study the flame propagation of premixed oxygen-rich combustion(oxygen enrichment coefficien...The investigation of syngas flame propagation has great benefits for the effective use of gas turbines.This essay sets out to study the flame propagation of premixed oxygen-rich combustion(oxygen enrichment coefficient in volumeΩ:0.21,0.27,0.32,0.37)of syngas(H_(2):CO=2:8)in half-closed rectangular ducts at elevated temperatures(T:300 K,400 K,500 K)and evaluate the effects of initial temperature and oxygen enrichment coefficient on the LBV from sensitivity analysis and kinetic analysis.This paper presents the effect of the expansion effect on laminar burning velocity for the first time,and separates the effect of the expansion effect on laminar burning velocity by a new method.Research shows that as the initial temperature goes up,the faster the exponential growth phase of the flame front velocity,the slower the slow growth phase.The smaller and earlier the maximum flame front velocity arrives,the slower the average flame speed is.As the oxygen enrichment coefficient goes up,the peak value of the flame front velocity gradually decreases.Oxygen-rich combustion and increasing initial temperature inhibit flame propagation in a half-open tube,but promote laminar burning velocity,which increases the key chemical bond and adiabatic flame temperature.The net reaction rate shows that oxygen-rich combustion mainly promotes the combustion reaction of H_(2)(R2).However,increasing the initial temperature mainly promoted the oxidation of CO(R29).Analysis of the reaction path showed that oxygen-rich combustion and increased initial temperature promoted the reaction of H_(2)and CO with key chemical bonds,increased OH concentration,and inhibited OH cracking reaction.展开更多
文摘Tumor invasion and metastases is one of the leading causes of tumor-related death while non-small cell lung cancer(NSCLC)is prone to multiple metastases.T umor microenvironment(TEM),especially extracellular matrix(ECM)and.fibroblasts,plays an important role in tumor metastases and invasion.To better understand the mechanism of NSCLC lung metastases and invasion,we constructed a three-dimensional(3D)co-culture model by seeding A549 tumor cells and human bronchial fibroblasts into decellularized lung scaffolds.Results showed that decellularized mice lung scaffolds retaining intact structure and ECM components,provided a suitable microenvironment for maintaining the viability,morphology of both cells in vitro.3D architecture and pulmonary ECM promoted the mobility/invasion ability of A549 cells by up-regulating the expression of matrix metalloproteinases(MMPs).Meanwhile,factors in TGF-1/Smad4 pathway were significantly up-regulated which may associated with epithelial-mesenchymal transition(EMT)of A549 cells.
基金supported by the National Natural Science Foundation of China(No.52004085)。
文摘The investigation of syngas flame propagation has great benefits for the effective use of gas turbines.This essay sets out to study the flame propagation of premixed oxygen-rich combustion(oxygen enrichment coefficient in volumeΩ:0.21,0.27,0.32,0.37)of syngas(H_(2):CO=2:8)in half-closed rectangular ducts at elevated temperatures(T:300 K,400 K,500 K)and evaluate the effects of initial temperature and oxygen enrichment coefficient on the LBV from sensitivity analysis and kinetic analysis.This paper presents the effect of the expansion effect on laminar burning velocity for the first time,and separates the effect of the expansion effect on laminar burning velocity by a new method.Research shows that as the initial temperature goes up,the faster the exponential growth phase of the flame front velocity,the slower the slow growth phase.The smaller and earlier the maximum flame front velocity arrives,the slower the average flame speed is.As the oxygen enrichment coefficient goes up,the peak value of the flame front velocity gradually decreases.Oxygen-rich combustion and increasing initial temperature inhibit flame propagation in a half-open tube,but promote laminar burning velocity,which increases the key chemical bond and adiabatic flame temperature.The net reaction rate shows that oxygen-rich combustion mainly promotes the combustion reaction of H_(2)(R2).However,increasing the initial temperature mainly promoted the oxidation of CO(R29).Analysis of the reaction path showed that oxygen-rich combustion and increased initial temperature promoted the reaction of H_(2)and CO with key chemical bonds,increased OH concentration,and inhibited OH cracking reaction.
