目的:探讨慢性心衰大鼠室旁核(PVN)内脂肪酸酰胺水解酶(FAAH)表达失衡导致中枢交感传出增加的机制。方法:雄性Wistar大鼠左冠状动脉结扎8周后,用超声心动图检测心功能,并通过组织病理学测定心肌梗死面积,鉴定大鼠心衰模型构建成功;酶联...目的:探讨慢性心衰大鼠室旁核(PVN)内脂肪酸酰胺水解酶(FAAH)表达失衡导致中枢交感传出增加的机制。方法:雄性Wistar大鼠左冠状动脉结扎8周后,用超声心动图检测心功能,并通过组织病理学测定心肌梗死面积,鉴定大鼠心衰模型构建成功;酶联免疫吸附法测血浆去甲肾上腺素(NE)水平;Western blot测定PVN内FAAH蛋白表达量;高效液相色谱法测定PVN内N-花生四烯酰乙醇胺(AEA)的生成量;PVN微量注射AEA、FAAH抑制剂PF3845或r AAV2-FAAH sh RNA病毒,记录交感驱动和心功能指标的变化。结果:与假手术组相比,心衰组心功能显著降低,血浆NE显著增加,PVN内FAAH表达量显著上调,AEA生成量显著减少(P<0.05);PVN微量灌注PF3845、AEA或r AAV2-FAAH sh RNA病毒后,心衰组交感驱动指标显著降低,心功能明显改善。结论:心衰状态下PVN内FAAH蛋白表达上调可能引起AEA生成量的减少,从而增强交感神经兴奋性,恶化心衰。展开更多
The maternal-fetal interface is a key barrier to protect the fetus from infection.Toll-like receptors (TLRs) at the maternal-fetal interface are involved in antiviral responses.TLRs are expressed in both maternal deci...The maternal-fetal interface is a key barrier to protect the fetus from infection.Toll-like receptors (TLRs) at the maternal-fetal interface are involved in antiviral responses.TLRs are expressed in both maternal decidua and fetal trophoblasts.Virus-induced activation of TLR signaling pathways triggers the release of interferon-related antiviral molecules and other inflammatory cytokines and/or chemokines by the host innate immune system,which may disrupt immune tolerance at the maternal-fetal interface and lead to pregnancy complications.In this review,we summarize the state of knowledge on the most common viral infections during pregnancy,antiviral TLR responses at the maternal-fetal interface,and TLR-associated pregnancy complications.展开更多
In order to rapidly and automatically identify the modulation level of digital amplitude modulated signals at low signal-to-noise ratio(SNR),a method of identifying the modulation levels of M-ary quadrature amplitude ...In order to rapidly and automatically identify the modulation level of digital amplitude modulated signals at low signal-to-noise ratio(SNR),a method of identifying the modulation levels of M-ary quadrature amplitude modulation(M-QAM)and M-ary amplitude shift keying(M-ASK)is proposed.In this method,wavelet transform with the optimal scale is used to identify the modulation levels of M-QAM and M-ASK signals.The performance of this method was investigated through simulations.Simulation results show that when the SNR is not lower than–4 dB,the percentage of correct identification of M-QAM is higher than 93%,and when the SNR is not lower than–10 dB,the percentage of correct identification of M-ASK is higher than 90%,using only 100 observed symbols.It shows that this method can rapidly acquire good performance at a low SNR.展开更多
文摘目的:探讨慢性心衰大鼠室旁核(PVN)内脂肪酸酰胺水解酶(FAAH)表达失衡导致中枢交感传出增加的机制。方法:雄性Wistar大鼠左冠状动脉结扎8周后,用超声心动图检测心功能,并通过组织病理学测定心肌梗死面积,鉴定大鼠心衰模型构建成功;酶联免疫吸附法测血浆去甲肾上腺素(NE)水平;Western blot测定PVN内FAAH蛋白表达量;高效液相色谱法测定PVN内N-花生四烯酰乙醇胺(AEA)的生成量;PVN微量注射AEA、FAAH抑制剂PF3845或r AAV2-FAAH sh RNA病毒,记录交感驱动和心功能指标的变化。结果:与假手术组相比,心衰组心功能显著降低,血浆NE显著增加,PVN内FAAH表达量显著上调,AEA生成量显著减少(P<0.05);PVN微量灌注PF3845、AEA或r AAV2-FAAH sh RNA病毒后,心衰组交感驱动指标显著降低,心功能明显改善。结论:心衰状态下PVN内FAAH蛋白表达上调可能引起AEA生成量的减少,从而增强交感神经兴奋性,恶化心衰。
基金This work was supported by by the grants from the Anti-COVID-19 Fund from International Peace Maternity and Child Health Hospital (GFY2020-COVID-19-01)the National Natural Science Foundation of China (81401274)the Interdisciplinary Program of Shanghai Jiao Tong University (YG2017ZD09 and YG2017MS40)。
文摘The maternal-fetal interface is a key barrier to protect the fetus from infection.Toll-like receptors (TLRs) at the maternal-fetal interface are involved in antiviral responses.TLRs are expressed in both maternal decidua and fetal trophoblasts.Virus-induced activation of TLR signaling pathways triggers the release of interferon-related antiviral molecules and other inflammatory cytokines and/or chemokines by the host innate immune system,which may disrupt immune tolerance at the maternal-fetal interface and lead to pregnancy complications.In this review,we summarize the state of knowledge on the most common viral infections during pregnancy,antiviral TLR responses at the maternal-fetal interface,and TLR-associated pregnancy complications.
文摘In order to rapidly and automatically identify the modulation level of digital amplitude modulated signals at low signal-to-noise ratio(SNR),a method of identifying the modulation levels of M-ary quadrature amplitude modulation(M-QAM)and M-ary amplitude shift keying(M-ASK)is proposed.In this method,wavelet transform with the optimal scale is used to identify the modulation levels of M-QAM and M-ASK signals.The performance of this method was investigated through simulations.Simulation results show that when the SNR is not lower than–4 dB,the percentage of correct identification of M-QAM is higher than 93%,and when the SNR is not lower than–10 dB,the percentage of correct identification of M-ASK is higher than 90%,using only 100 observed symbols.It shows that this method can rapidly acquire good performance at a low SNR.
