Objective Owing to antibiotic abuse and the subsequent development of antibiotic resistance,bacterial infection has become one of the most persistent unresolved problems.New antibacterial agents,especially those that ...Objective Owing to antibiotic abuse and the subsequent development of antibiotic resistance,bacterial infection has become one of the most persistent unresolved problems.New antibacterial agents,especially those that are environmental-friendly,are urgently needed.Methods Melanin extracted by filtration centrifugation and acid and proteolytic hydrolysis was characterized using UV,FTIR,TEM,and XPS.Photothermal conversion was calculated,and the bacteriostatic effects,in vitro and in vivo,were assessed by plate counting and ratios(%)of wound areas.Results Natural melanin hydrolyzed by trypsin had good photothermal conversion effects,which resulted in superior bacteriostatic activities.The extracted melanin along with laser NIR irradiation at808 nm promoted the healing of wounds infected by drug-resistant bacteria in vivo and was biocompatible according to toxicity tests in vivo and in vitro.Conclusion The present findings indicated a safe and efficient method of developing natural antibacterial agents.展开更多
基金the National Natural Science Foundation of China[No.21874079]Nature Science Foundation for Outstanding Young Scientists of Shandong Province[No.ZR2018JL011]+3 种基金Key R&D Project of Shandong Province[No.GG201809230180]Taishan Scholars Program of Shandong Province[No.tsqn201909088]Outstanding Youth Innovation Team of Universities in Shandong Province[No.2019KJA027]Science&Technology Fund Planning Project of Shandong Colleges and Universities[No.J16LA13&J18KA112]。
文摘Objective Owing to antibiotic abuse and the subsequent development of antibiotic resistance,bacterial infection has become one of the most persistent unresolved problems.New antibacterial agents,especially those that are environmental-friendly,are urgently needed.Methods Melanin extracted by filtration centrifugation and acid and proteolytic hydrolysis was characterized using UV,FTIR,TEM,and XPS.Photothermal conversion was calculated,and the bacteriostatic effects,in vitro and in vivo,were assessed by plate counting and ratios(%)of wound areas.Results Natural melanin hydrolyzed by trypsin had good photothermal conversion effects,which resulted in superior bacteriostatic activities.The extracted melanin along with laser NIR irradiation at808 nm promoted the healing of wounds infected by drug-resistant bacteria in vivo and was biocompatible according to toxicity tests in vivo and in vitro.Conclusion The present findings indicated a safe and efficient method of developing natural antibacterial agents.
