Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber （Cucumis sativus L.） in solar greenhouses in northern China. Here, two genotypes of cucumber...Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber （Cucumis sativus L.） in solar greenhouses in northern China. Here, two genotypes of cucumber （Deltastar and Jinyan 2） are used to determine the effect of low light stress on Rubisco expression and photosynthesis of leaves from emergence to senescence. During leaf development, the net photosynthetic rate （PN）, stomatal conductance （gs）, Rubisco initial activity and activation state, transcript levels of rbcL and rbcS, and the abundance of rbcL and rbcS DNA in these two genotypes increase rapidly to reach maximum in 10-20 d, and then decrease gradually. Meanwhile, the actual photosystem II efficiency （OpSll） of cucumber leaves slowly increased in the early leaf developing stages, but it declined quickly in leaf senescent stages, accompanied by an increased non-photochemical quenching （NPQ）. Moreover, PN, gs, initial Rubisco activity, and abundance of protein, mRNA and DNA of Rubisco subunits of leaves grown under 100 μmol m^-2 s^-1 are lower, and require more time to reach their maxima than those grown under 600 μmol m^-2 s^-1 during leaf development. All these results suggest that lower photosynthetic capacity of cucumber leaves from emergence to senescence under low light stress is probably due to down-regulated Rubisco gene expression in transcript and protein levels, and decreased initial and total activity as well as activation state of Rubisco. Deltastar performs better than Jinyan 2 under low light stress.展开更多
目的:探究骨桥蛋白(OPN)基因单核苷酸多态性(rs1126772、rs117291487、rs11730582、rs142608941、rs6813526)与男性弱精子症的关系。方法:选取135例弱精子症不育男性为病例组,239例有生育史的健康男性作为对照组,运用多重单碱基延伸PCR...目的:探究骨桥蛋白(OPN)基因单核苷酸多态性(rs1126772、rs117291487、rs11730582、rs142608941、rs6813526)与男性弱精子症的关系。方法:选取135例弱精子症不育男性为病例组,239例有生育史的健康男性作为对照组,运用多重单碱基延伸PCR技术(SNaPshot)对OPN基因rs1126772、rs117291487、rs11730582、rs142608941、rs6813526位点进行基因分型检测,分析这5个位点多态性与男性弱精子症的相关性。结果:OPN基因rs1126772位点GA基因型和A等位基因与弱精子症发病风险存在相关性(GA vs AA:OR=0.55,95%CI,0.35~0.86,P=0.009;A vs G:OR=0.64,95%CI,0.46~0.89,P=0.007);rs11730582位点CT基因型和T等位基因与弱精子症发病风险相关(CT vs TT:OR=0.526,95%CI,0.34~0.82,P=0.009;T vs C:OR=0.60,95%CI,0.44-0.83,P=0.002),单倍型分析发现,AATCT单倍型可能具有降低弱精子症的发病风险(AATCT:OR=0.61,95%CI,0.42~0.88,P=0.008)。结论:OPN基因rs1126772和rs11730582位点多态性可能与降低弱精子症的发生相关。展开更多
基金supported by China Agriculture Research System (CARS-25)the National Basic Research Program of China (973 Program, 2009CB11900)the National Key Technologies R&D Program of China (2011BAD12B01)
文摘Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber （Cucumis sativus L.） in solar greenhouses in northern China. Here, two genotypes of cucumber （Deltastar and Jinyan 2） are used to determine the effect of low light stress on Rubisco expression and photosynthesis of leaves from emergence to senescence. During leaf development, the net photosynthetic rate （PN）, stomatal conductance （gs）, Rubisco initial activity and activation state, transcript levels of rbcL and rbcS, and the abundance of rbcL and rbcS DNA in these two genotypes increase rapidly to reach maximum in 10-20 d, and then decrease gradually. Meanwhile, the actual photosystem II efficiency （OpSll） of cucumber leaves slowly increased in the early leaf developing stages, but it declined quickly in leaf senescent stages, accompanied by an increased non-photochemical quenching （NPQ）. Moreover, PN, gs, initial Rubisco activity, and abundance of protein, mRNA and DNA of Rubisco subunits of leaves grown under 100 μmol m^-2 s^-1 are lower, and require more time to reach their maxima than those grown under 600 μmol m^-2 s^-1 during leaf development. All these results suggest that lower photosynthetic capacity of cucumber leaves from emergence to senescence under low light stress is probably due to down-regulated Rubisco gene expression in transcript and protein levels, and decreased initial and total activity as well as activation state of Rubisco. Deltastar performs better than Jinyan 2 under low light stress.
文摘目的:探究骨桥蛋白(OPN)基因单核苷酸多态性(rs1126772、rs117291487、rs11730582、rs142608941、rs6813526)与男性弱精子症的关系。方法:选取135例弱精子症不育男性为病例组,239例有生育史的健康男性作为对照组,运用多重单碱基延伸PCR技术(SNaPshot)对OPN基因rs1126772、rs117291487、rs11730582、rs142608941、rs6813526位点进行基因分型检测,分析这5个位点多态性与男性弱精子症的相关性。结果:OPN基因rs1126772位点GA基因型和A等位基因与弱精子症发病风险存在相关性(GA vs AA:OR=0.55,95%CI,0.35~0.86,P=0.009;A vs G:OR=0.64,95%CI,0.46~0.89,P=0.007);rs11730582位点CT基因型和T等位基因与弱精子症发病风险相关(CT vs TT:OR=0.526,95%CI,0.34~0.82,P=0.009;T vs C:OR=0.60,95%CI,0.44-0.83,P=0.002),单倍型分析发现,AATCT单倍型可能具有降低弱精子症的发病风险(AATCT:OR=0.61,95%CI,0.42~0.88,P=0.008)。结论:OPN基因rs1126772和rs11730582位点多态性可能与降低弱精子症的发生相关。
