Computed tomography(CT) is one of the most commonly used non-invasive clinical imaging modalities to predict, diagnose and treat the disease. Iodinated contrast media(ICM) is a form of intravenous radiocontrast agent ...Computed tomography(CT) is one of the most commonly used non-invasive clinical imaging modalities to predict, diagnose and treat the disease. Iodinated contrast media(ICM) is a form of intravenous radiocontrast agent containing iodine, which enhances the visibility of hollow tissue structures in medical CT imaging. ICM may cause allergic reactions, contrast-induced nephropathy, hyperthyroidism and possibly metformin accumulation. It is significant to find out the risk factors, pathogenesis, diagnosis, prevention, and treatment of adverse reactions caused by ICM. Revealing the changes of the lipid droplets(LDs)viscosity in pathophysiological processes such as cancer and iodined contrast media induced adverse reaction is not only important for monitoring the occurrence and development of some pathophysiological processes but also vital for the deep insight of the biological effects of LDs in these pathophysiological processes. A lipid droplets targeted fluorescent probe DN-1 was devised to sense cellular viscosity alteration with high selectivity and sensitivity, which was applied to distinguish cancer cells and normal cells and reveal viscosity changes during iodined CT contrast media treatment.展开更多
The dynamics of plasma and shockwave expansion during two femtosecond laser pulse ablation of fused silica are studied using a time-resolved shadowgraph imaging technique. The experimental results reveal that during t...The dynamics of plasma and shockwave expansion during two femtosecond laser pulse ablation of fused silica are studied using a time-resolved shadowgraph imaging technique. The experimental results reveal that during the second pulse irradiation on the crater induced by the first pulse, the expansion of the plasma and shockwave is enhanced in the longitudinal direction. The plasma model and Fresnel diffraction theory are combined to calculate the laser intensity distribution by considering the change in surface morphology and transient material properties. The theoretical results show that after the free electron density induced by the rising edge of the pulse reaches the critical density, the originally transparent surface is transformed into a transient high-reflectivity surface(metallic state). Thus, the crater with a concave-lens-like morphology can tremendously reflect and refocus the latter part of the laser pulse, leading to a strong laser field with an intensity even higher than the incident intensity. This strong refocused laser pulse results in a stronger laser-induced air breakdown and enhances the subsequent expansion of the plasma and shockwave. In addition, similar shadowgraphs are also recorded in the single-pulse ablation of a concave microlens, providing experimental evidence for the enhancement mechanism.展开更多
Femtosecond laser-induced surface structures upon multiple pulses irradiation are strongly correlated with the pulse number,which in turn signifcantly afects successive laser-material interactions.By recording the dyn...Femtosecond laser-induced surface structures upon multiple pulses irradiation are strongly correlated with the pulse number,which in turn signifcantly afects successive laser-material interactions.By recording the dynamics of femtosecond laser ablation of silicon using time-resolved shadowgraphy,here we present direct visualization of the excitation of air plasma induced by the refected laser during the second pulse irradiation.Te interaction of the air plasma and silicon plasma is found to enhance the shockwave expansion induced by silicon ablation in the longitudinal direction,showing anisotropic expansion dynamics in diferent directions.We further demonstrate the vanishing of air plasma as the pulse number increases because of the generation of a rough surface without light focusing ability.In the scenario,the interaction of air plasma and silicon plasma disappears;the expansion of the silicon plasma and shockwave restores its original characteristic that is dominated by the laser-material coupling.Te results show that the excitation of air plasma and the laser-material coupling involved in laser-induced plasma and shockwave expansion are structure mediated and dependent on the pulse number,which is of fundamental importance for deep insight into the nature of laser-material interactions during multiple pulses ablation.展开更多
Available online The abnormal carboxylesterase(CES)expression is closely related to many diseases such as hyperlipidemia,atherosclerosis,obesity,liver cancer,type 2 diabetes mellitus and gastrointestinal stromal tumor...Available online The abnormal carboxylesterase(CES)expression is closely related to many diseases such as hyperlipidemia,atherosclerosis,obesity,liver cancer,type 2 diabetes mellitus and gastrointestinal stromal tumors.The detection of a single enzyme in practical samples is often constrained by the structural diversity of CESs.Thus,the development of broad-carboxylesterase responsive fluorescent probe,which can detect the presence of wide variety of CESs,may provide overall or category information from another point of view,supplementing the deficiency of single detection for CES subspecies.Organelle lysosome is involved in various cell processes,such as cell signaling,apoptosis,secretion,and energy metabolism.Up to date,lysosome-targeted fluorescent probes,especially those with red emission(over 550 nm,with relatively low biological harmfulness),for CES detection are still rare.A lysosomes-targeted red fluorescent probe CES-Lyso was designed to monitor intracellular a variety of carboxylesterases alteration with wonderful selectivity and sensitivity,which was further applied to distinguish different derived breast cancer cells and monitor carboxylesterase activity in the anticancer drug treatment.展开更多
基金the financial support from the National Natural Science Foundation of China (No. 21705120)the Project of Shandong Province Higher Educational Outstanding Youth Innovation Team (No. 2019KJM008)+1 种基金the Natural Science Foundation of Shandong Province,China (No. ZR^(2)017LB016)Foundation of Yuandu Scholar。
文摘Computed tomography(CT) is one of the most commonly used non-invasive clinical imaging modalities to predict, diagnose and treat the disease. Iodinated contrast media(ICM) is a form of intravenous radiocontrast agent containing iodine, which enhances the visibility of hollow tissue structures in medical CT imaging. ICM may cause allergic reactions, contrast-induced nephropathy, hyperthyroidism and possibly metformin accumulation. It is significant to find out the risk factors, pathogenesis, diagnosis, prevention, and treatment of adverse reactions caused by ICM. Revealing the changes of the lipid droplets(LDs)viscosity in pathophysiological processes such as cancer and iodined contrast media induced adverse reaction is not only important for monitoring the occurrence and development of some pathophysiological processes but also vital for the deep insight of the biological effects of LDs in these pathophysiological processes. A lipid droplets targeted fluorescent probe DN-1 was devised to sense cellular viscosity alteration with high selectivity and sensitivity, which was applied to distinguish cancer cells and normal cells and reveal viscosity changes during iodined CT contrast media treatment.
基金National Natural Science Foundation of China(NSFC)(51605029,91323301)
文摘The dynamics of plasma and shockwave expansion during two femtosecond laser pulse ablation of fused silica are studied using a time-resolved shadowgraph imaging technique. The experimental results reveal that during the second pulse irradiation on the crater induced by the first pulse, the expansion of the plasma and shockwave is enhanced in the longitudinal direction. The plasma model and Fresnel diffraction theory are combined to calculate the laser intensity distribution by considering the change in surface morphology and transient material properties. The theoretical results show that after the free electron density induced by the rising edge of the pulse reaches the critical density, the originally transparent surface is transformed into a transient high-reflectivity surface(metallic state). Thus, the crater with a concave-lens-like morphology can tremendously reflect and refocus the latter part of the laser pulse, leading to a strong laser field with an intensity even higher than the incident intensity. This strong refocused laser pulse results in a stronger laser-induced air breakdown and enhances the subsequent expansion of the plasma and shockwave. In addition, similar shadowgraphs are also recorded in the single-pulse ablation of a concave microlens, providing experimental evidence for the enhancement mechanism.
基金Tis research was supported by the National Key R&D Program of China(grant no.2017YFB1104300)and the National Natural Science Foundation of China(grant nos.91323301,11704028).
文摘Femtosecond laser-induced surface structures upon multiple pulses irradiation are strongly correlated with the pulse number,which in turn signifcantly afects successive laser-material interactions.By recording the dynamics of femtosecond laser ablation of silicon using time-resolved shadowgraphy,here we present direct visualization of the excitation of air plasma induced by the refected laser during the second pulse irradiation.Te interaction of the air plasma and silicon plasma is found to enhance the shockwave expansion induced by silicon ablation in the longitudinal direction,showing anisotropic expansion dynamics in diferent directions.We further demonstrate the vanishing of air plasma as the pulse number increases because of the generation of a rough surface without light focusing ability.In the scenario,the interaction of air plasma and silicon plasma disappears;the expansion of the silicon plasma and shockwave restores its original characteristic that is dominated by the laser-material coupling.Te results show that the excitation of air plasma and the laser-material coupling involved in laser-induced plasma and shockwave expansion are structure mediated and dependent on the pulse number,which is of fundamental importance for deep insight into the nature of laser-material interactions during multiple pulses ablation.
基金the financial support from the National Natural Science Foundation of China (No. 21705120)the Technology Support Project of Shandong Province Higher Educational Youth Innovation (No. 2019KJM008)+1 种基金the Natural Science Foundation of Shandong Province, China (No. ZR2017LB016)the Project of Shandong Province Higher Educational Science and Technology Program (No. J17KB074)
文摘Available online The abnormal carboxylesterase(CES)expression is closely related to many diseases such as hyperlipidemia,atherosclerosis,obesity,liver cancer,type 2 diabetes mellitus and gastrointestinal stromal tumors.The detection of a single enzyme in practical samples is often constrained by the structural diversity of CESs.Thus,the development of broad-carboxylesterase responsive fluorescent probe,which can detect the presence of wide variety of CESs,may provide overall or category information from another point of view,supplementing the deficiency of single detection for CES subspecies.Organelle lysosome is involved in various cell processes,such as cell signaling,apoptosis,secretion,and energy metabolism.Up to date,lysosome-targeted fluorescent probes,especially those with red emission(over 550 nm,with relatively low biological harmfulness),for CES detection are still rare.A lysosomes-targeted red fluorescent probe CES-Lyso was designed to monitor intracellular a variety of carboxylesterases alteration with wonderful selectivity and sensitivity,which was further applied to distinguish different derived breast cancer cells and monitor carboxylesterase activity in the anticancer drug treatment.