The rice false smut disease, caused by Ustilaginoidea virens, has emerged as a significantglobal threat to rice production. The mechanism of carbon catabolite repression plays a crucial role in theefficient utilizatio...The rice false smut disease, caused by Ustilaginoidea virens, has emerged as a significantglobal threat to rice production. The mechanism of carbon catabolite repression plays a crucial role in theefficient utilization of carbon nutrients and enzyme regulation in the presence of complex nutritionalconditions. Although significant progress has been made in understanding carbon catabolite repression infungi such as Aspergillus nidulans and Magnaporthe oryzae, its role in U. virens remains unclear. Toaddress this knowledge gap, we identified UvCreA, a pivotal component of carbon catabolite repression,in U. virens. Our investigation revealed that UvCreA localized to the nucleus. Deletion of UvCreA resultedin decreased growth and pathogenicity in U. virens. Through RNA-seq analysis, it was found that theknockout of UvCreA led to the up-regulation of 514 genes and down-regulation of 640 genes. Moreover,UvCreA was found to be involved in the transcriptional regulation of pathogenic genes and genesassociated with carbon metabolism in U. virens. In summary, our findings indicated that UvCreA isimportant in fungal development, virulence, and the utilization of carbon sources through transcriptionalregulation, thus making it a critical element of carbon catabolite repression.展开更多
目的探究安神补脑胶囊结合针刺对原发性失眠(心神不宁型)的临床研究。方法选取2021年6月—2022年6月收治的124例原发性失眠患者为研究对象。根据治疗方法分为观察组(n=64)与对照组(n=60),对照组采用针刺治疗,观察组在此基础上结合安神...目的探究安神补脑胶囊结合针刺对原发性失眠(心神不宁型)的临床研究。方法选取2021年6月—2022年6月收治的124例原发性失眠患者为研究对象。根据治疗方法分为观察组(n=64)与对照组(n=60),对照组采用针刺治疗,观察组在此基础上结合安神补脑胶囊治疗。采用阿森斯量表(Athens Insomnia Scale,AIS)、生活质量评分(the MOS item short from health survey,SF-36)量表、失眠严重指数量表(The insomnia severity index,ISI)记录两组患者治疗前后水平。观察组两组患者治疗前后血清指标肿瘤坏死因子(Tumor necrosis factor,TNF-α)、γ-氨基丁酸(γaminobutyric acid,GABA)、5-羟色胺(5-hydroxy tryptamine,5-HT)、核因子κB(nuclear factor kappa-B,NF-κB)含量水平变化。观察两组患者临床疗效和中医症候积分。结果两组患者治疗前入睡时间、白天情绪、白天身体功能、夜间苏醒、总睡眠时间、总睡眠质量、白天思睡评分、比期望的时间早醒评分无显著差异(P>0.05)。治疗后观察组入睡时间、白天情绪、白天身体功能、夜间苏醒、总睡眠时间、总睡眠质量、白天思睡评分、比期望的时间早醒评分显著低于对照组(P<0.05)。两组患者治疗前生理功能、身体疼痛、角色限制、总体健康、社会功能、活力、精神健康、情感职能无显著差异(P>0.05)。治疗后观察组生理功能、身体疼痛、角色限制、总体健康、社会功能、活力、精神健康、情感职能评分显著高于对照组(P<0.05)。两组患者治疗前ISI、中医症候积分无显著差异(P>0.05)。治疗后观察组ISI、中医症候积分较对照组显著降低(P<0.05)。两组患者治疗前TNF-α、NF-κB、GABA和5-HT水平无显著差异(P>0.05)。治疗后观察组TNF-α、NF-κB水平较对照组显著降低,且GABA和5-HT水平较对照组显著升高(P>0.05)。观察组总有效率为93.75%显著高于对照组81.67%(χ^(2)=4.252,P=0.039)。结论安神补脑胶囊结合针刺能有效改善血清因子水平,提升睡眠质量、提高临床疗效和生活质量。展开更多
基金the Key Projects of Zhejiang Provincial Natural Science Foundation,China(Grant No.LZ23C130002)the National Natural Science Foundation of China(Grant No.32100161)+3 种基金the Zhejiang Science and Technology Major Program on Rice New Variety Breeding,China(Grant No.2021C02063)the Key R&D Project of China National Rice Research Institute(Grant No.CNRRI-2020-04)the Chinese Academy of Agricultural Sciences under the Agricultural Sciences and Technologies Innovation Program,the Youth innovation Program of Chinese Academy of Agricultural Sciences(Grant No.Y2023QC22)the Joint Open Competitive Project of the Yazhou Bay Seed Laboratory and China National Seed Company Limited(Grant Nos.B23YQ1514 and B23CQ15EP).
文摘The rice false smut disease, caused by Ustilaginoidea virens, has emerged as a significantglobal threat to rice production. The mechanism of carbon catabolite repression plays a crucial role in theefficient utilization of carbon nutrients and enzyme regulation in the presence of complex nutritionalconditions. Although significant progress has been made in understanding carbon catabolite repression infungi such as Aspergillus nidulans and Magnaporthe oryzae, its role in U. virens remains unclear. Toaddress this knowledge gap, we identified UvCreA, a pivotal component of carbon catabolite repression,in U. virens. Our investigation revealed that UvCreA localized to the nucleus. Deletion of UvCreA resultedin decreased growth and pathogenicity in U. virens. Through RNA-seq analysis, it was found that theknockout of UvCreA led to the up-regulation of 514 genes and down-regulation of 640 genes. Moreover,UvCreA was found to be involved in the transcriptional regulation of pathogenic genes and genesassociated with carbon metabolism in U. virens. In summary, our findings indicated that UvCreA isimportant in fungal development, virulence, and the utilization of carbon sources through transcriptionalregulation, thus making it a critical element of carbon catabolite repression.
文摘目的探究安神补脑胶囊结合针刺对原发性失眠(心神不宁型)的临床研究。方法选取2021年6月—2022年6月收治的124例原发性失眠患者为研究对象。根据治疗方法分为观察组(n=64)与对照组(n=60),对照组采用针刺治疗,观察组在此基础上结合安神补脑胶囊治疗。采用阿森斯量表(Athens Insomnia Scale,AIS)、生活质量评分(the MOS item short from health survey,SF-36)量表、失眠严重指数量表(The insomnia severity index,ISI)记录两组患者治疗前后水平。观察组两组患者治疗前后血清指标肿瘤坏死因子(Tumor necrosis factor,TNF-α)、γ-氨基丁酸(γaminobutyric acid,GABA)、5-羟色胺(5-hydroxy tryptamine,5-HT)、核因子κB(nuclear factor kappa-B,NF-κB)含量水平变化。观察两组患者临床疗效和中医症候积分。结果两组患者治疗前入睡时间、白天情绪、白天身体功能、夜间苏醒、总睡眠时间、总睡眠质量、白天思睡评分、比期望的时间早醒评分无显著差异(P>0.05)。治疗后观察组入睡时间、白天情绪、白天身体功能、夜间苏醒、总睡眠时间、总睡眠质量、白天思睡评分、比期望的时间早醒评分显著低于对照组(P<0.05)。两组患者治疗前生理功能、身体疼痛、角色限制、总体健康、社会功能、活力、精神健康、情感职能无显著差异(P>0.05)。治疗后观察组生理功能、身体疼痛、角色限制、总体健康、社会功能、活力、精神健康、情感职能评分显著高于对照组(P<0.05)。两组患者治疗前ISI、中医症候积分无显著差异(P>0.05)。治疗后观察组ISI、中医症候积分较对照组显著降低(P<0.05)。两组患者治疗前TNF-α、NF-κB、GABA和5-HT水平无显著差异(P>0.05)。治疗后观察组TNF-α、NF-κB水平较对照组显著降低,且GABA和5-HT水平较对照组显著升高(P>0.05)。观察组总有效率为93.75%显著高于对照组81.67%(χ^(2)=4.252,P=0.039)。结论安神补脑胶囊结合针刺能有效改善血清因子水平,提升睡眠质量、提高临床疗效和生活质量。
