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星天牛成虫复眼外部形态和内部超微结构
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作者 文超 何金雨 +5 位作者 种海南 邱华龙 温秀军 王偲 温俊宝 肖海军 《环境昆虫学报》 CSCD 北大核心 2024年第3期773-780,共8页
星天牛Anoplophora chinensis能够取食上百种植物,以幼虫蛀干危害为主。本研究使用扫描电镜、透射电子显微镜和光学显微镜研究了星天牛成虫复眼外部形态和内部超微结构。结果表明星天牛复眼着生于触角基部,为不规则的肾形,表面光滑,由12... 星天牛Anoplophora chinensis能够取食上百种植物,以幼虫蛀干危害为主。本研究使用扫描电镜、透射电子显微镜和光学显微镜研究了星天牛成虫复眼外部形态和内部超微结构。结果表明星天牛复眼着生于触角基部,为不规则的肾形,表面光滑,由1211~1270个小眼组成,大部分小眼呈六边形,少数位于复眼边缘的小眼为不规则的五边形,小眼间角为6.90~9.56°。每个小眼由角膜、晶锥、视网膜细胞和基膜组成。角膜厚度为84.03±3.82μm,晶锥被初级色素细胞包围,色素颗粒直径为0.41~0.97μm。每个小眼包括8个视网膜细胞,其中6个周缘细胞(R1~R6)包围2个中央细胞(R7~R8),视网膜细胞的感杆小体在向心侧形成半融合的感杆束,次级色素细胞色素颗粒将相邻小眼的视网膜细胞隔离,色素颗粒直径为0.41~1.06μm。星天牛成虫复眼为并列像型,复眼分辨率较低,但视觉角度广,能够感受强度较低的光照。 展开更多
关键词 星天牛 复眼 视网膜细胞 视觉
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沟眶象味觉受体基因EscrGR8对雌成虫繁殖力的影响
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作者 郭晓丽 文超 +1 位作者 温俊宝 温晓健 《昆虫学报》 CAS CSCD 北大核心 2024年第3期307-317,共11页
【目的】分析和阐明沟眶象Eucryptorrhynchus scrobiculatus味觉受体基因EscrGR8的分子特性和功能,揭示其调节雌成虫繁殖力的作用。【方法】基于沟眶象触角转录组数据库,采用RACE克隆EscrGR8的cDNA全长序列;利用qRT-PCR检测EscrGR8在沟... 【目的】分析和阐明沟眶象Eucryptorrhynchus scrobiculatus味觉受体基因EscrGR8的分子特性和功能,揭示其调节雌成虫繁殖力的作用。【方法】基于沟眶象触角转录组数据库,采用RACE克隆EscrGR8的cDNA全长序列;利用qRT-PCR检测EscrGR8在沟眶象不同发育阶段(卵、1-6龄幼虫、蛹、雌成虫和雄成虫)和雌雄成虫组织(去除触角和喙的头、触角、口器、中肠、前足、睾丸、卵巢、雄虫交配器和雌虫产卵器)中的表达量。显微注射雌成虫dsRNA后0,6,12,24,36,48和72 h时利用qRT-PCR检测RNAi后EscrGR8的表达量。检测显微注射dsEscrGR8后1-5 d和6-11 d时沟眶象雌成虫在不同土壤含水量(0~10%,11%~20%,21%~40%,41%~60%,61%~80%和81%~100%)条件下的产卵选择率、总产卵数量和所产卵的孵化率,研究抑制EscrGR8表达对雌成虫产卵选择性和繁殖力的影响。【结果】成功克隆了沟眶象EscrGR8(GenBank登录号:OR836580)的cDNA全长序列,开放阅读框(ORF)长1251 bp,编码了416个氨基酸,具有6个跨膜结构域。多序列比对和系统发育进化分析结果表明,EscrGR8与其他昆虫的GR的氨基酸序列同源性普遍较低,其中与棉铃象甲Anthonomus grandis的AgraGR64f氨基酸序列一致性为30.96%。qRT-PCR检测结果表明,EscrGR8在沟眶象不同发育阶段和成虫组织中的表达量具有显著差异,EscrGR8在雌成虫中的表达量最高,在卵中的表达量最低;EscrGR8在雌成虫卵巢中特异性高表达。显微注射dsEscrGR8不仅在一定时间内显著降低EscrGR8的表达量,并且对雌成虫的产卵选择性具有显著影响。显微注射dsEscrGR8后1-5 d时沟眶象雌成虫在含水量21%~40%土壤条件下的产卵选择率和所产卵的孵化率与显微注射dsGFP的对照组相比分别显著降低了24.66%和15.83%;在含水量81%~100%土壤条件下的产卵选择率与显微注射dsGFP的对照组相比显著增加了28.39%,雌成虫所产卵几乎不孵化。【结论】本研究证实了味觉受体基因EscrGR8对沟眶象雌成虫产卵选择和繁殖力的影响,有助于理解昆虫味觉受体基因的多样性和功能特异性。 展开更多
关键词 沟眶象 产卵量 味觉受体基因 RNAI 表达模式
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视觉信号在昆虫检测植物寄主中的作用 被引量:1
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作者 文超 马涛 +4 位作者 王偲 梁仕萍 朱映 温俊宝 温秀军 《环境昆虫学报》 CSCD 北大核心 2020年第3期607-614,共8页
植物为数十万种昆虫提供各种资源,如食物、交配、产卵和躲避天敌的场所。目前对昆虫检测植物寄主的研究主要关注昆虫嗅觉系统和植物寄主挥发物之间的相互作用,对昆虫视觉系统发挥的作用关注较少。近年来,对昆虫视觉器官、光行为反应及... 植物为数十万种昆虫提供各种资源,如食物、交配、产卵和躲避天敌的场所。目前对昆虫检测植物寄主的研究主要关注昆虫嗅觉系统和植物寄主挥发物之间的相互作用,对昆虫视觉系统发挥的作用关注较少。近年来,对昆虫视觉器官、光行为反应及分子生物学的研究表明,昆虫具有优异的视觉能力,能够辨别植物寄主的颜色、大小和轮廓,应该将视觉纳入昆虫检测植物寄主的研究中。昆虫能够利用视觉信号准确检测寄主,远距离时,主要依靠植物寄主轮廓检测寄主,近距离时,寄主的大小、颜色和形状发挥重要作用。利用昆虫视觉识别寄主的专一性研制诱捕装置,可为害虫的监测和防治提供一定的理论基础。 展开更多
关键词 昆虫 视觉 植物寄主 检测 光学信号
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Effective accumulated temperature and developmental threshold temperature for Semanotus bifasciatus(Motschulsky) in Beijing 被引量:6
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作者 MA Li-qin GAO Shi-ji +2 位作者 wen jun-bao ZONG Shi-xiang XU Zhi-chun 《Forestry Studies in China》 CAS 2008年第2期125-129,共5页
In order to understand the occurrence and development of Semanotus bifasciatus (Motschulsky), a borer insect of Platycladus orientalis in parts of northern and eastern China and to improve its prediction, the develo... In order to understand the occurrence and development of Semanotus bifasciatus (Motschulsky), a borer insect of Platycladus orientalis in parts of northern and eastern China and to improve its prediction, the developmental threshold temperature (C1) and effective accumulated temperature (K) of S. bifasciatus were determined under conditions of constant and variable temperatures. The results show that under constant temperatures the value of C1 has a range of 8.90±1.77℃and the value of K lies between 95.19±13.14 degree-day for eggs; the values for larvae are C1= 13.26±3.06℃ and the number of degree-day is K 2,885.07±187.87 degree-day; for pupae the ranges are C1 = 8.93±2.49℃ and K= 131.20±25.63 degree-day. Under the condition of ambient temperatures, the values for eggs are C1= 17.33±1.24℃ and K= 70.79±8.99 degree-day. It is suggested that S. bifasciatus adults over-winter in a state of dormancy, not at a diapause. Warm winter may accelerate the life cycle ofS. bifasciatus. Equations can be used to predict an early occurrence ofS. bifasciatus. 展开更多
关键词 Semanotus bifasciatus (Motschulsky) developmental threshold temperature effective accumulated temperature eggs larvae PUPAE
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Seasonal diet of the Great Spotted Woodpecker(Picoides major) in shelterwood plantations of Wulate Qianqi County,Inner Mongolia
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作者 JIAO Zhen-biao WAN Tao +4 位作者 wen jun-bao HU Jia-fu LUO You-qing FU Lin-ju Zhang Lin-sheng 《Forestry Studies in China》 CAS 2008年第2期119-124,共6页
The feeding habits of the Great Spotted Woodpecker (Picoides major) were surveyed by field observations and trace checking from 2005 to 2007, in Wulate Qianqi County, Inner Mongolia Autonomous Region, China, during ... The feeding habits of the Great Spotted Woodpecker (Picoides major) were surveyed by field observations and trace checking from 2005 to 2007, in Wulate Qianqi County, Inner Mongolia Autonomous Region, China, during which the woodpecker performed fourteen types of foraging techniques. Pecking and hammering were most common in winter; gleaning and probing were most common at times of high food availability at the trunk and tree branches during the breeding season and in summer. The woodpecker gleans arthropods and insect larvae in the breeding season and summer, seeds and nuts mainly in the winter and pokes holes for sap-sucking in winter. The main diet consisted of arthropods and plant seeds. The diet was significantly different between seasons The Great Spotted Woodpecker primarily consumes defoliators and the food on tree trunks from March to October. The contribution of wood borers to the diet was higher in winter, lower in the breeding season and summer. The proportion of the food on ground was small in the breeding season and high in the Summer and winter. 展开更多
关键词 Picoides major food habit foraging technique wood borer DEFOLIATOR
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Chemical control of Apriona germari(Hope) larvae with zinc phosphide sticks
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作者 SHUI Sheng-ying wen jun-bao +3 位作者 CHEN Min HU Xiao-li LIU Fang LI Jing 《Forestry Studies in China》 CAS 2009年第1期9-13,共5页
Larvae of Apriona germari (Hope), found widely in China, cause serious damage to trees and are difficult to control. Since some biological control methods have not been applied effectively in the field, chemical con... Larvae of Apriona germari (Hope), found widely in China, cause serious damage to trees and are difficult to control. Since some biological control methods have not been applied effectively in the field, chemical control methods are used widely. Because of low cost, no poisonous residues and relatively safe application, zinc phosphide sticks have been tested to control the larvae as a matter of urgency. However, there were no criteria about the dosage and use. Given the characteristics of larvae tunnels on triploid Populus tomentosa trees and the one-on-one way of using poison-fumigated sticks, the larvae were treated by three treatments with zinc phosphide sticks. The treatments are as follows: inserting one stick into the first hole of the tunnel from bottom to top (T1), inserting a stick into each of the lowest two holes (T2) and inserting one stick into the first and third hole from bottom to top separately (T3). We cleared the frass around the holes and on the ground before inserting the sticks and blocking every hole with mud for each treatment. After the fourth investigation was carried out, the larvae of CK were still active, and had caused serious damage and emanated fresh fi'ass from old and new holes. Some larvae treated with T1 and T2 were in an intermediate state, but all of the larvae treated with T3 died completely and their old holes had healed. The results show that inserting zinc phosphide sticks (the extent of dispersion of the sticks was 20%) is a feasible method to control A. germari (Hope) larvae in triploid P. tomentosa of pulpwood trees. Because there were no statistically significant differences (p 〉 0.05) among the three treatments, we recommend that T1 should be used for economic efficacy. 展开更多
关键词 Apriona germari LARVAE zinc phosphide sticks chemical control
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论民主革命时期马克思主义中国化主体发展的认识成果
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作者 闻君宝 《大庆师范学院学报》 2020年第1期26-33,共8页
马克思主义中国化主体,是推动马克思主义中国化实践中最活跃、最关键的要素。马克思主义中国化的产生和发展,正是通过以中国共产党为核心的马克思主义中国化主体为媒介来实现的。其自身发展的如何,直接影响着马克思主义中国化的进程和... 马克思主义中国化主体,是推动马克思主义中国化实践中最活跃、最关键的要素。马克思主义中国化的产生和发展,正是通过以中国共产党为核心的马克思主义中国化主体为媒介来实现的。其自身发展的如何,直接影响着马克思主义中国化的进程和兴衰。民主革命时期,随着马克思主义中国化实践的展开,主体对于自身发展问题的认识逐渐清晰和明确,实际围绕主体发展的对象、基本范畴、主体地位的实现和作用的发挥等形成了一系列积极的认识成果。这些认识成果的取得和发展,不仅成为同时代推动主体自身发展和成熟的基本思想指导,为实际解决关乎中国革命的一系列实践问题提供了重要的理论支持,也对新时代条件下深化主体与马克思主义中国化的关系,更好地推动当代马克思主义中国化实践等具有重要价值。 展开更多
关键词 民主革命 马克思主义中国化主体 毛泽东
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昆虫复眼结构及视觉导航研究进展 被引量:12
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作者 文超 马涛 +3 位作者 王偲 温俊宝 季英超 温秀军 《应用昆虫学报》 CAS CSCD 北大核心 2019年第1期28-36,共9页
复眼是昆虫主要的视觉器官,具有感受物体的大小、形状和颜色等功能。研究复眼结构是了解昆虫感光机制的基础,进而明确视觉信号在昆虫行为中的作用。昆虫的复眼包括了重叠像眼和并列像眼两种类型,重叠像眼具有聚光作用,可以感受到低强度... 复眼是昆虫主要的视觉器官,具有感受物体的大小、形状和颜色等功能。研究复眼结构是了解昆虫感光机制的基础,进而明确视觉信号在昆虫行为中的作用。昆虫的复眼包括了重叠像眼和并列像眼两种类型,重叠像眼具有聚光作用,可以感受到低强度的光,但是分辨率较低;并列像眼没有聚光作用,分辨率较高。大多数白天活动的昆虫具有并列像眼,夜晚活动的昆虫具有重叠像眼。分子生物学、视网膜电位和行为学研究结果均表明昆虫对紫外、蓝和绿光最敏感。昆虫利用天体和地面目标物信号定向和导航,依靠天体发出的偏振光和地面目标物与背景颜色的对比度进行视觉判断,对于任何一种昆虫,这两种信号都可能同时应用。视觉信号在昆虫定位植物寄主、寻找配偶和产卵过程中起着至关重要的作用,不能低估昆虫视觉能力的作用。利用昆虫对不同波长光波的趋性,研制专一性强的诱捕装置,可为害虫的无公害防治提供理论基础。 展开更多
关键词 光感受器 复眼 视觉 导航 定位
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