利用福建省近岸海域1959—2018年的赤潮历史记录数据资料,在地理信息系统(Geographical Information System,GIS)平台下采用核密度估计函数(kernel density estimation)对赤潮历史记录数据中的赤潮发生频次、赤潮发生持续时间、赤潮面...利用福建省近岸海域1959—2018年的赤潮历史记录数据资料,在地理信息系统(Geographical Information System,GIS)平台下采用核密度估计函数(kernel density estimation)对赤潮历史记录数据中的赤潮发生频次、赤潮发生持续时间、赤潮面积和赤潮藻类毒性等参数进行了空间评估分析并根据赤潮灾害危险度区划方法得到未来5 a福建省近岸海域赤潮灾害危险性空间分布情况,同时创新性地提出赤潮危险性区划结果的验证方法。危险性区划结果表明未来5 a福建省近岸海域处于极高危险性区的海域主要有霞浦县近岸海域、连江县近岸海域、平潭县近岸东部海域及厦门市同安湾近岸海域。处于高危险性区的海域主要有福鼎市-霞浦县近岸海域、罗源县-连江县近岸海域、平潭县周边近岸海域东部海域、福清市南部-莆田东北部近岸海域、石狮市周边近岸海域及厦门市近岸海域,其余海域处于中危险性区和低危险性区。验证结果表明构建的区划方法和区划结果具有较好的合理性和可信性,区划结果将为福建省近岸海域赤潮灾害应急处置、纳污海域污染总量控制制定有效的管理措施提供科学依据,从而达到防灾减灾的目的,而且对指导海洋水产养殖、滨海旅游业规划等具有重要的科学意义。展开更多
The paper was to study the effects of different concentrations of lipopelysaccharide （LPS） on expression of nuclear factor Kappa B （NF-κB） and lingual antimicrobial peptide （LAP） gene in mammary epithelial ceil...The paper was to study the effects of different concentrations of lipopelysaccharide （LPS） on expression of nuclear factor Kappa B （NF-κB） and lingual antimicrobial peptide （LAP） gene in mammary epithelial ceils of dairy cow. The mammary epithelial ceils of dairy cow were stimulated by different concentrations （50, 100,200,400 and 800 ng/mL） of LPS. The total RNA of cells was extracted after stimulation for 2, 4, 8, 16, 24, 48 and 72 h, respectively, and the mRNA expression levels of NF-κB P65 and LAP were evaluated by real-time quantitative PCR. The results showed that the expression of NF-κB P65 and LAP mRNA treated with 400 ng/mL LPS for 72 h were the highest compared to the control group （ P 〈0.01 ）. The result confirmed that the expression activity of NF-κB was enhanced in inflammatory effects of inammary epithelial cells induced by LPS, which regulated the expression of defense gene LAP, with certain dose and time effects.展开更多
Mapping regional spatial patterns of coral reef geomorphology provides the primary information to understand the constructive processes in the reef ecosystem. However, this work is challenged by the pixel-based image ...Mapping regional spatial patterns of coral reef geomorphology provides the primary information to understand the constructive processes in the reef ecosystem. However, this work is challenged by the pixel-based image classification method for its comparatively low accuracy. In this paper, an object-based image analysis(OBIA)method was presented to map intra-reef geomorphology of coral reefs in the Xisha Islands, China using Landsat 8satellite imagery. Following the work of the Millennium Coral Reef Mapping Project, a regional reef class hierarchy with ten geomorphic classes was first defined. Then, incorporating the hierarchical concept and integrating the spectral and additional spatial information such as context, shape and contextual relationships, a large-scale geomorphic map was produced by OBIA with accuracies generally more than 80%. Although the robustness of OBIA has been validated in the applications of coral reef mapping from individual reefs to reef system in this paper, further work is still required to improve its transferability.展开更多
文摘利用福建省近岸海域1959—2018年的赤潮历史记录数据资料,在地理信息系统(Geographical Information System,GIS)平台下采用核密度估计函数(kernel density estimation)对赤潮历史记录数据中的赤潮发生频次、赤潮发生持续时间、赤潮面积和赤潮藻类毒性等参数进行了空间评估分析并根据赤潮灾害危险度区划方法得到未来5 a福建省近岸海域赤潮灾害危险性空间分布情况,同时创新性地提出赤潮危险性区划结果的验证方法。危险性区划结果表明未来5 a福建省近岸海域处于极高危险性区的海域主要有霞浦县近岸海域、连江县近岸海域、平潭县近岸东部海域及厦门市同安湾近岸海域。处于高危险性区的海域主要有福鼎市-霞浦县近岸海域、罗源县-连江县近岸海域、平潭县周边近岸海域东部海域、福清市南部-莆田东北部近岸海域、石狮市周边近岸海域及厦门市近岸海域,其余海域处于中危险性区和低危险性区。验证结果表明构建的区划方法和区划结果具有较好的合理性和可信性,区划结果将为福建省近岸海域赤潮灾害应急处置、纳污海域污染总量控制制定有效的管理措施提供科学依据,从而达到防灾减灾的目的,而且对指导海洋水产养殖、滨海旅游业规划等具有重要的科学意义。
基金Supported by National Natural Science Foundation of China(30460093,31060328)Projects for Key Areas of Scientific Research in Inner Mongolia Colleges and Universities(ZL010144)Major Projects for Cultivation of New Breeds of Genetically Modified Organisms(2008ZX08007-004)
文摘The paper was to study the effects of different concentrations of lipopelysaccharide （LPS） on expression of nuclear factor Kappa B （NF-κB） and lingual antimicrobial peptide （LAP） gene in mammary epithelial ceils of dairy cow. The mammary epithelial ceils of dairy cow were stimulated by different concentrations （50, 100,200,400 and 800 ng/mL） of LPS. The total RNA of cells was extracted after stimulation for 2, 4, 8, 16, 24, 48 and 72 h, respectively, and the mRNA expression levels of NF-κB P65 and LAP were evaluated by real-time quantitative PCR. The results showed that the expression of NF-κB P65 and LAP mRNA treated with 400 ng/mL LPS for 72 h were the highest compared to the control group （ P 〈0.01 ）. The result confirmed that the expression activity of NF-κB was enhanced in inflammatory effects of inammary epithelial cells induced by LPS, which regulated the expression of defense gene LAP, with certain dose and time effects.
基金The National Natural Science Foundation of China under contract No.41201328the Science Foundation for Young Scholars of China’s State Oceanic Administration under contract No.2013415
文摘Mapping regional spatial patterns of coral reef geomorphology provides the primary information to understand the constructive processes in the reef ecosystem. However, this work is challenged by the pixel-based image classification method for its comparatively low accuracy. In this paper, an object-based image analysis(OBIA)method was presented to map intra-reef geomorphology of coral reefs in the Xisha Islands, China using Landsat 8satellite imagery. Following the work of the Millennium Coral Reef Mapping Project, a regional reef class hierarchy with ten geomorphic classes was first defined. Then, incorporating the hierarchical concept and integrating the spectral and additional spatial information such as context, shape and contextual relationships, a large-scale geomorphic map was produced by OBIA with accuracies generally more than 80%. Although the robustness of OBIA has been validated in the applications of coral reef mapping from individual reefs to reef system in this paper, further work is still required to improve its transferability.
