Based on Mohandas＇ work, a new animal model of red blood cell(RBC) synchronous senescence in a near normal physiological conditions was proposed. Using this model,we studied the changes of RBC surface charge with the...Based on Mohandas＇ work, a new animal model of red blood cell(RBC) synchronous senescence in a near normal physiological conditions was proposed. Using this model,we studied the changes of RBC surface charge with the aging process. The results showed that its surface charge decreased with RBC agingprocess. Nevertheless,the regulation of the changes was not linear during RBC aging. The indexes of (DI)d and (DI)or describing RBC rheological behaviours duringits aging process were measured with a new Ektacytometry. It is demonstrated thatboth the indexes decreased with RBC aging,but the relationship was not linear. Thecharacteristics of the indexes displayed a common regularity of smaller alterationsin the first half period of RBC life span and greater alterations in the latter periodof RBC life span. It was also shown that the regulation of the changes for each individual index were not alike. The alterations of RBC mechanical properties duringlife span maight result from the influence of RBC shape, the properties of cytoplasm and membrane viscoelasity and the other factors during RBC aging.展开更多
After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of t...After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of the liver and spleen, and the hemorhelogical indexes within 4 weeks. The results indicated that after injecting MEL cells, the mice entered into a pathological status similar to erythroleukemia, which had the following exhibitions: the tissue structures of the liver and spleen were damaged, a mass of proerythroblasts, basophil erythroblasts and polychromatophilic erythroblasts could be observed on the smears of the bone marrow and spleen, and the deformability and orientation ability of erythrocytes were both depressed. The pathogenicity of MEL-TF19 cells carrying TFAR19 gene was obviously lower than that of MEL cells, and the MEL-TF19 cells even lost their faintish pathogenicity under the apop-tosis-inducing effect of the chemotherapeutic reagent. The outcome from the animal experiments suggests that the TFAR19 gene suppresses the pathogenicity of MEL cells to the mice, and the effect may be better exerted with the synergy of the chemotherapeutic reagent.展开更多
文摘Based on Mohandas＇ work, a new animal model of red blood cell(RBC) synchronous senescence in a near normal physiological conditions was proposed. Using this model,we studied the changes of RBC surface charge with the aging process. The results showed that its surface charge decreased with RBC agingprocess. Nevertheless,the regulation of the changes was not linear during RBC aging. The indexes of (DI)d and (DI)or describing RBC rheological behaviours duringits aging process were measured with a new Ektacytometry. It is demonstrated thatboth the indexes decreased with RBC aging,but the relationship was not linear. Thecharacteristics of the indexes displayed a common regularity of smaller alterationsin the first half period of RBC life span and greater alterations in the latter periodof RBC life span. It was also shown that the regulation of the changes for each individual index were not alike. The alterations of RBC mechanical properties duringlife span maight result from the influence of RBC shape, the properties of cytoplasm and membrane viscoelasity and the other factors during RBC aging.
基金the National Natural Science Foundation of China (Grant Nos. 30270355 & 10572007)
文摘After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of the liver and spleen, and the hemorhelogical indexes within 4 weeks. The results indicated that after injecting MEL cells, the mice entered into a pathological status similar to erythroleukemia, which had the following exhibitions: the tissue structures of the liver and spleen were damaged, a mass of proerythroblasts, basophil erythroblasts and polychromatophilic erythroblasts could be observed on the smears of the bone marrow and spleen, and the deformability and orientation ability of erythrocytes were both depressed. The pathogenicity of MEL-TF19 cells carrying TFAR19 gene was obviously lower than that of MEL cells, and the MEL-TF19 cells even lost their faintish pathogenicity under the apop-tosis-inducing effect of the chemotherapeutic reagent. The outcome from the animal experiments suggests that the TFAR19 gene suppresses the pathogenicity of MEL cells to the mice, and the effect may be better exerted with the synergy of the chemotherapeutic reagent.
