Effects of a Regional Chinese Diet and Its Vitamin Supplementation on Proliferation of Human Esophageal Cancer Cell Lines

Objective To study the effects of a local diet popular in Yanting region(YT diet) on the proliferation of two human cell lines(Eca-109 esophageal squamous cell carcinoma line and HL7702 normal liver epithelial cell line) in rats by a sero-physiological approach.Methods Male SD rats were divided into six groups and fed respectively with a conventional diet and the YT diet(one of the five experimental diets) supplemented with two vitamin mixtures(Mix.1:vitamins A,E,and folic acid;Mix.2:mix.1 plus riboflavin and vitamin C) at two different doses.On the 30th day,sera were collected from the rats and added into a medium for cell culture,with 10% FBS used as a serum control.The effects were assessed by MTT assay,DNA synthesis and flow cytometry assays.Results Compared with the control,the sera from rats fed with the YT diet significantly promoted the proliferation of Eca-109 cells,which was,however,reversed by the supplementation with two vitamin mixtures at high doses.Surprisingly,the same treatment produced contrary effects on HL7702 cells as compared with Eca-109 cells.Conclusion The sera from rats fed with the YT diet could promote the proliferation of human esophageal cancer cell line Eca-109,whereas the sera from those fed with the YT diet supplemented with vitamin mixtures might have inhibitory effects on the proliferation of Eca-109 cells.

Study of the effect of Jian-Pi-Xiao-Shi formula on infantile rats with anorexia

Background:The objective of this study was to study the effect of Chinese patent drug Jian-Pi-Xiao-Shi formula on rats with infantile anorexia.Methods:The Sprague Dawley rats with 4 weeks old were divided into 5 groups:normal group,model group,large,medium,and small doses of sample group.Twelve rats were included in each group with half males and half females.In addition to the normal group,the rats in each group were free to take the special feed during 28 consecutive days,which results in the pretended anorexia model,being the rats in the normal group free to take the ordinary feed.Except for the normal group and the model group,the other groups were given Chinese patent drug Jian-Pi-Xiao-Shi formula by a gavage of 1 mL/100g once a day for 28 days.The distilled water was given to the normal group and the model group under the same conditions.After 28 days,the contents of theβ-endorphin,cholecystokinin octapeptide,gastrin,motilin and pepsin were measured.Results:The results demonstrated that the Chinese patent drug Jian-Pi-Xiao-Shi formula could significantly increase the body weight and the food intake of the infantile rats with anorexia,increase the pepsin activity,the content of motilin and the expression ofβ-endorphin,and in the other hand decrease the expression of the cholecystokinin.Conclusion:The Chinese patent drug Jian-Pi-Xiao-Shi formula can promote the appetite and the digestive function of infantile rats with anorexia.This study suggested that the Chinese patent drug Jian-Pi-Xiao-Shi formula can enhance the appetite of the children and to improve the overall digestive function.