A leukocyte segmentation method based on S component and B component images is proposed.Threshold segmentation operation is applied to get two binary images in S component and B component images.The samples used in th...A leukocyte segmentation method based on S component and B component images is proposed.Threshold segmentation operation is applied to get two binary images in S component and B component images.The samples used in this study are peripheral blood smears.It is easy tofind from the two binary images that gray values are the same at every corresponding pixels in theleukocyte cytoplasm region,but opposite in the other regions.The feature shows that "IMAGEAND"operation can be employed on the two binary images to segment the cytoplasm region ofleukocyte.By doing"IMAGE XOR"operation between cytoplasn region and nucleus region,theleukocyte segment ation can be retrieved effectively.The segmentation accuracy is evaluated by comparing the segmentation result of the proposed method with the manual segmentation by ahematologist.Experiment results show that the proposed method is of a higher segmentationaccuracy and it also performs well when leukocytes overlap_with erythrocytes.The averagesegmentation accuracy of the proposed method reaches 97.7%for segmenting five types ofleukocyte.Good segmentation results provide an important foundation for leukocytes aut omaticrecognition.展开更多
文摘A leukocyte segmentation method based on S component and B component images is proposed.Threshold segmentation operation is applied to get two binary images in S component and B component images.The samples used in this study are peripheral blood smears.It is easy tofind from the two binary images that gray values are the same at every corresponding pixels in theleukocyte cytoplasm region,but opposite in the other regions.The feature shows that "IMAGEAND"operation can be employed on the two binary images to segment the cytoplasm region ofleukocyte.By doing"IMAGE XOR"operation between cytoplasn region and nucleus region,theleukocyte segment ation can be retrieved effectively.The segmentation accuracy is evaluated by comparing the segmentation result of the proposed method with the manual segmentation by ahematologist.Experiment results show that the proposed method is of a higher segmentationaccuracy and it also performs well when leukocytes overlap_with erythrocytes.The averagesegmentation accuracy of the proposed method reaches 97.7%for segmenting five types ofleukocyte.Good segmentation results provide an important foundation for leukocytes aut omaticrecognition.