The stripe disease-specific protein (SP) encoded by the rice stripe virus (RSV) was successfully used as a localization signal of the virus in its vector, the small brown lanthopper, Laodelphax striatellus Fallen. Imm...The stripe disease-specific protein (SP) encoded by the rice stripe virus (RSV) was successfully used as a localization signal of the virus in its vector, the small brown lanthopper, Laodelphax striatellus Fallen. Immunogold particles in large numbers were detected in various parts of the viruliferous females: the ovum, surface of chorion, the mid-gut lumen, and the columnar cells. Whereas there was noneof these particles in the non-viruliferous females and males, and testis of viruliferous males. Endosymbionts (mycetocytes) were abundant, harboring ovaries of both viruliferous and non-viruliferous females, but none in the testis of males. The results provided us with the direct proof that RSV is a ciruculative and propagative plant virus and it was transovarially transmitted alongside with endosymbionts of its vector. Therefore, we deem it a nice lead for future studies on the mechanism of RSV transmission and functioning of its viral proteins.展开更多
Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and...Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and S52.In the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair.The constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci.Based on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).展开更多
Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of ...Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 9 OsHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phyto-hormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes (OsHsp72.90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHsp18.03) showed high expression in the endosperm, indicating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes (OsHsp93.04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHsp18.03) and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.展开更多
基金This work was supported by the McKnight Foundation (Grant No. 14001404).
文摘The stripe disease-specific protein (SP) encoded by the rice stripe virus (RSV) was successfully used as a localization signal of the virus in its vector, the small brown lanthopper, Laodelphax striatellus Fallen. Immunogold particles in large numbers were detected in various parts of the viruliferous females: the ovum, surface of chorion, the mid-gut lumen, and the columnar cells. Whereas there was noneof these particles in the non-viruliferous females and males, and testis of viruliferous males. Endosymbionts (mycetocytes) were abundant, harboring ovaries of both viruliferous and non-viruliferous females, but none in the testis of males. The results provided us with the direct proof that RSV is a ciruculative and propagative plant virus and it was transovarially transmitted alongside with endosymbionts of its vector. Therefore, we deem it a nice lead for future studies on the mechanism of RSV transmission and functioning of its viral proteins.
基金This work was support ed by the Shanghai Municipal Scientifi c&Technological Commission(Grant Nos.033107019)Shanghai Municipal Agricultural Commission(Grant Nos 2003-2-1-2).
文摘Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and S52.In the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair.The constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci.Based on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).
基金supported by the National Program on Research and Development of Transgenic Plants of China(2008ZX08001-001)the Key Project of Shanghai Municipal Agriculture Commission(2009-6-4-2)+1 种基金the Shanghai Municipal Science and Technology CommissionShanghai Postdoctoral Fund(10R21420400)
文摘Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 9 OsHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phyto-hormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes (OsHsp72.90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHsp18.03) showed high expression in the endosperm, indicating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes (OsHsp93.04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHsp18.03) and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.
