A safe,economical treatment of hazardous chromium-bearing vanadate residue(CVR)will significantly benefit the clean production of chromate-bearing salts.This study investigated recovery of sodium vanadate and sodium c...A safe,economical treatment of hazardous chromium-bearing vanadate residue(CVR)will significantly benefit the clean production of chromate-bearing salts.This study investigated recovery of sodium vanadate and sodium chromate from CVR in sodium bicarbonate solution.Results indicate that the stability of calcium vanadate and calcium chromate depends on pH and[HCO3?].CaV2O6?4H2O transforms into CaV2O6?4H2O,CaV2O6?2H2O,CaV2O6,Ca2V2O7?2H2O,and Ca5(VO4)3(OH)when pH increases from 7.51 to 12.32.Increasing pH and reducing CVR dosage improve the vanadate extraction rate,and high V2O5 and Na2Cr2O7?2H2O extraction rates are achieved in dilute NaHCO3 solution.Moreover,addition of NaOH positively contributes to the recovery of vanadate and chromate from CVR.Over 95%V2O5 and Na2Cr2O7?2H2O in CVR can be extracted from 60 g/L NaHCO3 and 30 g/L NaOH solutions at 90°C for 2 h.In order to reduce the hazardous residue containing chromate after recovery of CVR,calcium circulation is presented.Results show that more than 60%lime can be saved with fresh residue addition to remove vanadate from sodium chromate solution due to the active CaCO3.Moreover,no lime is required in removal of vanadate when the roasting residue is added.Therefore,a novel process is developed for utilization of CVR.展开更多
目的:探讨人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、突触素(Synaptophysin)、基质金属蛋白酶-9(MMP-9)血管内皮生长因子(VEGF)表达的影响。方法:将大鼠C6胶质瘤细胞分为对照组、人参皂苷Rh2低剂量组(16μg/m L)、人参皂苷Rh2中剂量组(32μ...目的:探讨人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、突触素(Synaptophysin)、基质金属蛋白酶-9(MMP-9)血管内皮生长因子(VEGF)表达的影响。方法:将大鼠C6胶质瘤细胞分为对照组、人参皂苷Rh2低剂量组(16μg/m L)、人参皂苷Rh2中剂量组(32μg/m L)、人参皂苷Rh2高剂量组(48μg/m L),CCK-8法和平板克隆实验检测细胞增殖;流式细胞术检测细胞凋亡;Transwell检测细胞侵袭;实时荧光定量-聚合酶链式反应(qRT-PCR)检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9 m RNA表达;蛋白质印迹法(Western blot)检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9蛋白表达。结果:与对照组比较,人参皂苷Rh2低剂量组、人参皂苷Rh2中剂量组、人参皂苷Rh2高剂量组大鼠C6胶质瘤细胞OD_(450)值(24 h、48 h)、克隆形成率、细胞侵袭数、VEGF、Synaptophysin、MMP-9 m RNA及蛋白表达降低,细胞凋亡率、Siah-1 m RNA及蛋白表达升高,且呈剂量依赖性(P<0.05)。结论:人参皂苷Rh2可能通过上调Siah-1,下调VEGF、Synaptophysin、MMP-9表达来抑制大鼠C6胶质瘤细胞增殖与侵袭,促进细胞凋亡。展开更多
基金Project(51274242)supported by the National Natural Science Foundation of ChinaProject(2015CX001)supported by the Innovation-driven Plan of Central South University,China
文摘A safe,economical treatment of hazardous chromium-bearing vanadate residue(CVR)will significantly benefit the clean production of chromate-bearing salts.This study investigated recovery of sodium vanadate and sodium chromate from CVR in sodium bicarbonate solution.Results indicate that the stability of calcium vanadate and calcium chromate depends on pH and[HCO3?].CaV2O6?4H2O transforms into CaV2O6?4H2O,CaV2O6?2H2O,CaV2O6,Ca2V2O7?2H2O,and Ca5(VO4)3(OH)when pH increases from 7.51 to 12.32.Increasing pH and reducing CVR dosage improve the vanadate extraction rate,and high V2O5 and Na2Cr2O7?2H2O extraction rates are achieved in dilute NaHCO3 solution.Moreover,addition of NaOH positively contributes to the recovery of vanadate and chromate from CVR.Over 95%V2O5 and Na2Cr2O7?2H2O in CVR can be extracted from 60 g/L NaHCO3 and 30 g/L NaOH solutions at 90°C for 2 h.In order to reduce the hazardous residue containing chromate after recovery of CVR,calcium circulation is presented.Results show that more than 60%lime can be saved with fresh residue addition to remove vanadate from sodium chromate solution due to the active CaCO3.Moreover,no lime is required in removal of vanadate when the roasting residue is added.Therefore,a novel process is developed for utilization of CVR.
文摘目的:探讨人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、突触素(Synaptophysin)、基质金属蛋白酶-9(MMP-9)血管内皮生长因子(VEGF)表达的影响。方法:将大鼠C6胶质瘤细胞分为对照组、人参皂苷Rh2低剂量组(16μg/m L)、人参皂苷Rh2中剂量组(32μg/m L)、人参皂苷Rh2高剂量组(48μg/m L),CCK-8法和平板克隆实验检测细胞增殖;流式细胞术检测细胞凋亡;Transwell检测细胞侵袭;实时荧光定量-聚合酶链式反应(qRT-PCR)检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9 m RNA表达;蛋白质印迹法(Western blot)检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9蛋白表达。结果:与对照组比较,人参皂苷Rh2低剂量组、人参皂苷Rh2中剂量组、人参皂苷Rh2高剂量组大鼠C6胶质瘤细胞OD_(450)值(24 h、48 h)、克隆形成率、细胞侵袭数、VEGF、Synaptophysin、MMP-9 m RNA及蛋白表达降低,细胞凋亡率、Siah-1 m RNA及蛋白表达升高,且呈剂量依赖性(P<0.05)。结论:人参皂苷Rh2可能通过上调Siah-1,下调VEGF、Synaptophysin、MMP-9表达来抑制大鼠C6胶质瘤细胞增殖与侵袭,促进细胞凋亡。
