The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulce...The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulcer model was established by oral administration of 30mgkg^(-1) IDM after 7 days of TH-GL or omeprazole(OME)administration in rats.Then the macroscopic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E_(2)(PGE_(2)),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were analyzed.The experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model group.Compared to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats.In addition,TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at normal levels.TH-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glutathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory factor.In conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory response.Therefore,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.展开更多
目的探讨瑞芬太尼对女性晕动病患者术后恶心呕吐的影响。方法选择2019年9月—2020年12月本院收治的有晕动病病史拟行择期无痛胃镜检查的患者(美国麻醉协会(ASA)Ⅰ-Ⅱ级、年龄20-50岁、体重指数18-30 kg/m2)120例纳入研究,随机数表法分...目的探讨瑞芬太尼对女性晕动病患者术后恶心呕吐的影响。方法选择2019年9月—2020年12月本院收治的有晕动病病史拟行择期无痛胃镜检查的患者(美国麻醉协会(ASA)Ⅰ-Ⅱ级、年龄20-50岁、体重指数18-30 kg/m2)120例纳入研究,随机数表法分为丙泊酚+瑞芬太尼组(PR组)、丙泊酚+舒芬太尼组(PS组)和丙泊酚组(P组)共三组,每组各40例。主要观察指标为术后24 h PONV情况;次要观察指标为患者离开PACU时眩晕情况、丙泊酚总用量、恢复时间及患者满意度。结果最终有116例患者纳入统计分析,PR组38例,PS组39例,P组39例。术后24 h PONV发生情况:三组间差异具有统计学意义(P<0.05);PS组发生情况明显高于PR组和P组,差异有统计学意义(P<0.05);PR组和P组两组间差异无统计学意义(P>0.05);丙泊酚总用量比较:P组丙泊总酚用量最大,P组分别和PR、PS组比较,差异有统计学意义(P<0.05);PR组用量次之,与PS组比较,差异有统计学意义(P<0.05);恢复时间:P组恢复时间明显长于PR、PS组,差异有统计学意义(P<0.05),而PR组和PS组两组之间则差异无统计学意义(P>0.05);患者离开PACU时眩晕情况:PS组明显高于PR组和P组,差异有统计学意义(P<0.05),PR和P组两组之间差异无统计学意义(P>0.05);满意度比较:PS组满意度明显低于PR组和P组,差异有统计学意义(P<0.05),PR和P组两组之间差异无统计学意义(P>0.05)。结论女性晕动病患者行门诊胃肠镜检查时选择丙泊酚复合0.5μg/kg瑞芬太尼安全、有效,并能有效降低患者PONV,提高患者满意度,值得在临床推广应用。展开更多
基金supported by the National Key R&D Pro-grams of China(No.2018YFD0901103)the Hainan Provincial Natural Science Foundation of China(No.2019 RC093).
文摘The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulcer model was established by oral administration of 30mgkg^(-1) IDM after 7 days of TH-GL or omeprazole(OME)administration in rats.Then the macroscopic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E_(2)(PGE_(2)),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were analyzed.The experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model group.Compared to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats.In addition,TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at normal levels.TH-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glutathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory factor.In conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory response.Therefore,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.
文摘目的探讨瑞芬太尼对女性晕动病患者术后恶心呕吐的影响。方法选择2019年9月—2020年12月本院收治的有晕动病病史拟行择期无痛胃镜检查的患者(美国麻醉协会(ASA)Ⅰ-Ⅱ级、年龄20-50岁、体重指数18-30 kg/m2)120例纳入研究,随机数表法分为丙泊酚+瑞芬太尼组(PR组)、丙泊酚+舒芬太尼组(PS组)和丙泊酚组(P组)共三组,每组各40例。主要观察指标为术后24 h PONV情况;次要观察指标为患者离开PACU时眩晕情况、丙泊酚总用量、恢复时间及患者满意度。结果最终有116例患者纳入统计分析,PR组38例,PS组39例,P组39例。术后24 h PONV发生情况:三组间差异具有统计学意义(P<0.05);PS组发生情况明显高于PR组和P组,差异有统计学意义(P<0.05);PR组和P组两组间差异无统计学意义(P>0.05);丙泊酚总用量比较:P组丙泊总酚用量最大,P组分别和PR、PS组比较,差异有统计学意义(P<0.05);PR组用量次之,与PS组比较,差异有统计学意义(P<0.05);恢复时间:P组恢复时间明显长于PR、PS组,差异有统计学意义(P<0.05),而PR组和PS组两组之间则差异无统计学意义(P>0.05);患者离开PACU时眩晕情况:PS组明显高于PR组和P组,差异有统计学意义(P<0.05),PR和P组两组之间差异无统计学意义(P>0.05);满意度比较:PS组满意度明显低于PR组和P组,差异有统计学意义(P<0.05),PR和P组两组之间差异无统计学意义(P>0.05)。结论女性晕动病患者行门诊胃肠镜检查时选择丙泊酚复合0.5μg/kg瑞芬太尼安全、有效,并能有效降低患者PONV,提高患者满意度,值得在临床推广应用。
