目的观察比较植物精油抑制痤疮丙酸杆菌的活性初步预测潜在活性成分及其作用机制。方法MIC试验考察植物精油中的枳实和紫苏精油的抑菌活性选取抑菌活性好的精油进行网络药理学的作用机制预测。TCMSP及Swiss Target Prediction数据库得...目的观察比较植物精油抑制痤疮丙酸杆菌的活性初步预测潜在活性成分及其作用机制。方法MIC试验考察植物精油中的枳实和紫苏精油的抑菌活性选取抑菌活性好的精油进行网络药理学的作用机制预测。TCMSP及Swiss Target Prediction数据库得到植物精油的活性成分及其靶点,Gene Cards获得痤疮丙酸杆菌相关靶点,建立“化合物-靶点-通路”网络和“蛋白间相互作用”网络得到关键靶点,GO富集和KEGG通路注释得到相关通路。结果枳实精油的最小抑菌浓度(MIC)为8μL/mL而紫苏精油抑菌活性不佳。网络药理学结果表明,枳实精油抑制痤疮丙酸杆菌的核心靶点为CYP19A1,作用最强的蛋白为VEGFA,核心成分为圣草酚、黄柏酮、6-甲氧基酸橙皮油素;潜在通路为positive regulation of gene expression、negative regulation of transcription from RNA polymeraseⅡpromoter、cytoplasm、zinc ion binding以及pathways in cancer等。结论枳实中的圣草酚、黄柏酮、6-甲氧基酸橙皮油素通过positive regulation of gene expression等通路作用于CYP19A1靶点达到抑制痤疮丙酸杆菌的作用。展开更多
Magnolia grandiflora is an important medicinal, ornamental and horticultural plant species. The chloroplast （cp） genome of M. grandiflora was sequenced using a 454 sequencing platform and the genome structure was co...Magnolia grandiflora is an important medicinal, ornamental and horticultural plant species. The chloroplast （cp） genome of M. grandiflora was sequenced using a 454 sequencing platform and the genome structure was compared with other related species. The complete cp genome ofM. grandiflora was 159623 bp in length and contained a pair of inverted repeats （IR） of 26563 bp separated by large and small single copy （LSC, SSC） regions of 87757 and 18740 bp, respectively. A total of 129 genes were successfully annotated, 18 of which included introns. The identity, number and GC content of M. grandiflora cp genes were similar to those of other Magnoliaceae species genomes. Analysis revealed 218 simple sequence repeat （SSR） loci, most composed of A or T, contributing to a bias in base composition. The types and abundances of repeat units in Magnoliaceae species were relatively conserved and these loci will be useful for developing M. grandiflora cp genome vectors. In addition, results indicated that the cp genome size in Magnoliaceae species and the position of the IR border were closely related to the length of the ycfl gene. Phylogenetic analyses based on 66 shared genes from 30 species using maximum parsimony （MP） and max- imum likelihood （ML） methods provided strong support for the phylogenetic position of Magnolia. The availability of the complete cp genome sequence of M. grandiflora provides valuable information for breeding of desirable varieties, cp genetic engineering, developing useful molecular markers and phylogenetic analyses in Magnoliaceae.展开更多
OBJECTIVE: To evaluate the effect of compound Gaoziban tablet(复方高滋斑片, CGZBT) on depression, and to investigate the underlying mechanism. METHODS: The components of CGZBT were analysed by high-performance liquid ...OBJECTIVE: To evaluate the effect of compound Gaoziban tablet(复方高滋斑片, CGZBT) on depression, and to investigate the underlying mechanism. METHODS: The components of CGZBT were analysed by high-performance liquid chromatography. Then, we assessed the effects of varying doses of CGZBT on an established chronic unpredictable mild stress(CUMS) model in rats. Whether animals were depressed was evaluated by sucrose preference test, open field test and forced swimming test. Neurotransmitters of hippocampus were detected by liquid chromatography-mass spectrometry. Serum levels of tumor necrosis factor-alpha(TNF-α), interleukin(IL)-1β, IL-6, IL-4, and IL-10 were measured by enzyme-linked immunosorbent assay. Expressions of toll-like receptor 4(TLR4), myeloid differentiation factor 88(My D88), phospho-nuclear factorkappa B(p-NF-κB), cyclooxygenase-2(COX-2), ionized calcium binding adapter molecule-1(IBA-1) were assessed by immunohistochemical staining and western blotting. RESULTS: Eight compounds were identified from CGZBT, moreover, our results showed that CGZBT effectively reversed the CUMS-induced decrease in sucrose preference, shortened the movement distance and prolonged immobility time. CGZBT significantly increased levels of 5-hydroxytryptamine, dopamine, norepinephrine, 5-hydroxyindoleacetic acid levels, and reduced the expression of TNF-α, IL-1β, IL-6, yet increased IL-4 and IL-10. Furthermore, the expressions of TLR4, My D88, COX-2, p-NF-κB and IBA-1 in hippocampus were effectively reversed after treatment with CGZBT. CONCLUSIONS: These results indicated that CGZBT could, at least in part, alleviate depression induced by CUMS via the TLR4/My D88/NF-κB pathway, suggesting its potential as an antidepressant drug.展开更多
目的构建半夏抗癌的成分-靶点-通路"网络,探究半夏可能抗癌活性成分及其潜在作用机制。方法通过文献和TCMSP数据库查找半夏化学成分采用类药性(DL)以及口服生物利用度(OB)评估筛选活性成分;根据反向药效团匹配方法,利用Drugbank、P...目的构建半夏抗癌的成分-靶点-通路"网络,探究半夏可能抗癌活性成分及其潜在作用机制。方法通过文献和TCMSP数据库查找半夏化学成分采用类药性(DL)以及口服生物利用度(OB)评估筛选活性成分;根据反向药效团匹配方法,利用Drugbank、Pharmmapper数据库筛选半夏活性成分潜在靶点基因,通过GeneCards和MalaCards数据库得到癌症相关基因靶点;将活性成分的潜在靶点基因与癌症相关基因进行比对,得到半夏抗癌的潜在靶点基因,使用KOBAS3.0数据库进行KEGG通路注释和GO富集采用Cytoscape3.6.1软件构建半夏抗癌的成分-靶点-通路"网络。结果半夏可能通过豆甾醇、黄芩苷等10个活性成分,作用于包括EGFR、AKT1 等20个基因靶点从而调节Pathways in cancer、Proteoglycans in cancer、Endocrine resistance等32条信号通路共同起到抗癌的效果。结论构建的"成分-靶点-通路"网络图揭示了半夏通过多成分、多靶点、多通路的形式发挥抗癌作用的特点,为进一步阐释半夏抗癌作用机制及其开发利用提供了科学依据。展开更多
Coded excitation technology （CET） can effectively enhance the penetration and resolution of ultrasonic testing. To analyze the influence of rock properties on pulse compression performance （PCP） of coded excitatio...Coded excitation technology （CET） can effectively enhance the penetration and resolution of ultrasonic testing. To analyze the influence of rock properties on pulse compression performance （PCP） of coded excitation signals （CES）, a numerical simulation, and an ultrasonic experiment on different rock samples are performed; and the detection ability of several CESs are also investigated and compared. The results of experiments showed that the loss of the signal-to-noise ratio （SNR） of Barker coded signal with tapered linear frequency modulated carrier （BTLFM） is always less than Barker coded signal with sine carrier （BS）, while the resolution loss of BTLFM is lower than tapered linear frequency modulated signal （TLFM）. In sum, the results not only verifiy the effectiveness of CET, but also provide a basis for the parameter settings of coded signals used in rock ultrasonic testing.展开更多
OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, ...OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, and kidney tissue extracts.METHODS: Rats with UC induced by TNBS enema were used as models in this study. Metabolic profiles of the three tissues were analyzed and pathway analysis of biomarkers was performed after CSD administration and further integration of metabolic networks.RESULTS: Thirteen biomarkers were screened from colon, liver, and kidney tissue extracts, and the levels of these substances were up-or down-regulated in the model group, but their levels were reversed after CSD administration. These biomarkers were mainly related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Glutathione metabolism, Arachidonic acid metabolism, Nicotinate and nicotinamide metabolism,Alanine, aspartate and glutamate metabolism.CONCLUSION: CSD could significantly ameliorate the symptoms of UC by regulating multiple metabolic pathways.展开更多
文摘目的观察比较植物精油抑制痤疮丙酸杆菌的活性初步预测潜在活性成分及其作用机制。方法MIC试验考察植物精油中的枳实和紫苏精油的抑菌活性选取抑菌活性好的精油进行网络药理学的作用机制预测。TCMSP及Swiss Target Prediction数据库得到植物精油的活性成分及其靶点,Gene Cards获得痤疮丙酸杆菌相关靶点,建立“化合物-靶点-通路”网络和“蛋白间相互作用”网络得到关键靶点,GO富集和KEGG通路注释得到相关通路。结果枳实精油的最小抑菌浓度(MIC)为8μL/mL而紫苏精油抑菌活性不佳。网络药理学结果表明,枳实精油抑制痤疮丙酸杆菌的核心靶点为CYP19A1,作用最强的蛋白为VEGFA,核心成分为圣草酚、黄柏酮、6-甲氧基酸橙皮油素;潜在通路为positive regulation of gene expression、negative regulation of transcription from RNA polymeraseⅡpromoter、cytoplasm、zinc ion binding以及pathways in cancer等。结论枳实中的圣草酚、黄柏酮、6-甲氧基酸橙皮油素通过positive regulation of gene expression等通路作用于CYP19A1靶点达到抑制痤疮丙酸杆菌的作用。
基金supported by the National Natural Science Foundation of China (30970307, 81130069)the Innovation Research Team of the University of Ministry of Education of China (IRT1150)
文摘Magnolia grandiflora is an important medicinal, ornamental and horticultural plant species. The chloroplast （cp） genome of M. grandiflora was sequenced using a 454 sequencing platform and the genome structure was compared with other related species. The complete cp genome ofM. grandiflora was 159623 bp in length and contained a pair of inverted repeats （IR） of 26563 bp separated by large and small single copy （LSC, SSC） regions of 87757 and 18740 bp, respectively. A total of 129 genes were successfully annotated, 18 of which included introns. The identity, number and GC content of M. grandiflora cp genes were similar to those of other Magnoliaceae species genomes. Analysis revealed 218 simple sequence repeat （SSR） loci, most composed of A or T, contributing to a bias in base composition. The types and abundances of repeat units in Magnoliaceae species were relatively conserved and these loci will be useful for developing M. grandiflora cp genome vectors. In addition, results indicated that the cp genome size in Magnoliaceae species and the position of the IR border were closely related to the length of the ycfl gene. Phylogenetic analyses based on 66 shared genes from 30 species using maximum parsimony （MP） and max- imum likelihood （ML） methods provided strong support for the phylogenetic position of Magnolia. The availability of the complete cp genome sequence of M. grandiflora provides valuable information for breeding of desirable varieties, cp genetic engineering, developing useful molecular markers and phylogenetic analyses in Magnoliaceae.
基金Supported by the Scientific Research Project of Education Department of Hubei Province (D20192003):Synthesis and Anti-hepatocellular Carcinoma Effect of Novel NMT1 Inhibitors。
文摘OBJECTIVE: To evaluate the effect of compound Gaoziban tablet(复方高滋斑片, CGZBT) on depression, and to investigate the underlying mechanism. METHODS: The components of CGZBT were analysed by high-performance liquid chromatography. Then, we assessed the effects of varying doses of CGZBT on an established chronic unpredictable mild stress(CUMS) model in rats. Whether animals were depressed was evaluated by sucrose preference test, open field test and forced swimming test. Neurotransmitters of hippocampus were detected by liquid chromatography-mass spectrometry. Serum levels of tumor necrosis factor-alpha(TNF-α), interleukin(IL)-1β, IL-6, IL-4, and IL-10 were measured by enzyme-linked immunosorbent assay. Expressions of toll-like receptor 4(TLR4), myeloid differentiation factor 88(My D88), phospho-nuclear factorkappa B(p-NF-κB), cyclooxygenase-2(COX-2), ionized calcium binding adapter molecule-1(IBA-1) were assessed by immunohistochemical staining and western blotting. RESULTS: Eight compounds were identified from CGZBT, moreover, our results showed that CGZBT effectively reversed the CUMS-induced decrease in sucrose preference, shortened the movement distance and prolonged immobility time. CGZBT significantly increased levels of 5-hydroxytryptamine, dopamine, norepinephrine, 5-hydroxyindoleacetic acid levels, and reduced the expression of TNF-α, IL-1β, IL-6, yet increased IL-4 and IL-10. Furthermore, the expressions of TLR4, My D88, COX-2, p-NF-κB and IBA-1 in hippocampus were effectively reversed after treatment with CGZBT. CONCLUSIONS: These results indicated that CGZBT could, at least in part, alleviate depression induced by CUMS via the TLR4/My D88/NF-κB pathway, suggesting its potential as an antidepressant drug.
文摘目的构建半夏抗癌的成分-靶点-通路"网络,探究半夏可能抗癌活性成分及其潜在作用机制。方法通过文献和TCMSP数据库查找半夏化学成分采用类药性(DL)以及口服生物利用度(OB)评估筛选活性成分;根据反向药效团匹配方法,利用Drugbank、Pharmmapper数据库筛选半夏活性成分潜在靶点基因,通过GeneCards和MalaCards数据库得到癌症相关基因靶点;将活性成分的潜在靶点基因与癌症相关基因进行比对,得到半夏抗癌的潜在靶点基因,使用KOBAS3.0数据库进行KEGG通路注释和GO富集采用Cytoscape3.6.1软件构建半夏抗癌的成分-靶点-通路"网络。结果半夏可能通过豆甾醇、黄芩苷等10个活性成分,作用于包括EGFR、AKT1 等20个基因靶点从而调节Pathways in cancer、Proteoglycans in cancer、Endocrine resistance等32条信号通路共同起到抗癌的效果。结论构建的"成分-靶点-通路"网络图揭示了半夏通过多成分、多靶点、多通路的形式发挥抗癌作用的特点,为进一步阐释半夏抗癌作用机制及其开发利用提供了科学依据。
基金supported by the National Natural Science Foundation of China(41104117)
文摘Coded excitation technology （CET） can effectively enhance the penetration and resolution of ultrasonic testing. To analyze the influence of rock properties on pulse compression performance （PCP） of coded excitation signals （CES）, a numerical simulation, and an ultrasonic experiment on different rock samples are performed; and the detection ability of several CESs are also investigated and compared. The results of experiments showed that the loss of the signal-to-noise ratio （SNR） of Barker coded signal with tapered linear frequency modulated carrier （BTLFM） is always less than Barker coded signal with sine carrier （BS）, while the resolution loss of BTLFM is lower than tapered linear frequency modulated signal （TLFM）. In sum, the results not only verifiy the effectiveness of CET, but also provide a basis for the parameter settings of coded signals used in rock ultrasonic testing.
基金Supported by the Chinese National Natural Science Foundation (Immunomodulatory Mechanism of Bmsc Evs Mediated by Ephb2/Ephrin-b1 Pathway in Ulcerative Colitis and Synergistic Effect of Compound Sophorae decoction, No.81774093)。
文摘OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, and kidney tissue extracts.METHODS: Rats with UC induced by TNBS enema were used as models in this study. Metabolic profiles of the three tissues were analyzed and pathway analysis of biomarkers was performed after CSD administration and further integration of metabolic networks.RESULTS: Thirteen biomarkers were screened from colon, liver, and kidney tissue extracts, and the levels of these substances were up-or down-regulated in the model group, but their levels were reversed after CSD administration. These biomarkers were mainly related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Glutathione metabolism, Arachidonic acid metabolism, Nicotinate and nicotinamide metabolism,Alanine, aspartate and glutamate metabolism.CONCLUSION: CSD could significantly ameliorate the symptoms of UC by regulating multiple metabolic pathways.
