目的 通过比较不同统计学方案对精液检验室间质评结果的影响,选择合适的统计学方案进行推广。方法 以精子浓度为例,选择2022年湖南省20家实验室精液室间质评数据,分别用传统统计方案、稳健统计方案以及结合稳健统计技术剔除“离群值”...目的 通过比较不同统计学方案对精液检验室间质评结果的影响,选择合适的统计学方案进行推广。方法 以精子浓度为例,选择2022年湖南省20家实验室精液室间质评数据,分别用传统统计方案、稳健统计方案以及结合稳健统计技术剔除“离群值”后的传统统计进行分析比对,比较其优劣。结果 4组精子浓度数据经传统统计无法剔除“离群值”,与Robust稳健统计方法或结合稳健统计技术剔除“离群值”后的传统统计室间质评结果有差异,传统统计与稳健统计合格实验室数分别为:19 vs 16、19 vs 16、19 vs 19、19 vs 19。结论 传统统计方法不适用于不符合正态分布、数据量较小、可能存在较多“离群值”的精液检验室间质评统计,推荐使用Robust稳健统计方法;或结合稳健统计技术剔除“离群值”后,可得到与稳健统计方案相似的结果。展开更多
The type Ⅱ toxin-antitoxin genes are responsible for the phenotypic switch to a quasi-dormant state that enables cell survival under stresses,a similar function to heterocyst of cyanobacteria. In this paper,we partic...The type Ⅱ toxin-antitoxin genes are responsible for the phenotypic switch to a quasi-dormant state that enables cell survival under stresses,a similar function to heterocyst of cyanobacteria. In this paper,we particularly study the role of gene pair all3211-asl3212 under Spectinomycin stress to reveal how the type Ⅱ toxin-antitoxin involved in environmental stress responses. Bioinformatics prediction shows that toxin protein gene All3211 is homologous to Maz F,a member of maz EF family that encoding nucleases. We clone gene all3211-asl3212 into expression vectors to identify its molecular characteristics. Deletion mutant strains of all3211-asl3212 are selected in a tri-parental mating screen. Phenotype comparisons of mutant and wild type reveals no difference of single-deletion-mutants in pigment integrity,the sensitivity to antibiotics,and heterocyst formation. The results show that deletion mutation of single TAS gene pair all3211-asl3212 results in limited effects on the cellular growth of PCC 7120. Thus,we suggest that dosage compensating might be provided from redundant genes or bypass pathways to offset obvious phenotypic differences.展开更多
文摘目的 通过比较不同统计学方案对精液检验室间质评结果的影响,选择合适的统计学方案进行推广。方法 以精子浓度为例,选择2022年湖南省20家实验室精液室间质评数据,分别用传统统计方案、稳健统计方案以及结合稳健统计技术剔除“离群值”后的传统统计进行分析比对,比较其优劣。结果 4组精子浓度数据经传统统计无法剔除“离群值”,与Robust稳健统计方法或结合稳健统计技术剔除“离群值”后的传统统计室间质评结果有差异,传统统计与稳健统计合格实验室数分别为:19 vs 16、19 vs 16、19 vs 19、19 vs 19。结论 传统统计方法不适用于不符合正态分布、数据量较小、可能存在较多“离群值”的精液检验室间质评统计,推荐使用Robust稳健统计方法;或结合稳健统计技术剔除“离群值”后,可得到与稳健统计方案相似的结果。
基金Supported by the National Natural Science Foundation of China(31001099/C190101)Central University Natural Science Foundation of China(CJSl3003,CJS13004)Key Laboratory of Microbiology and Biotrans Formation Funded Projects of South-Central University for Nationalities(XJS09002)
文摘The type Ⅱ toxin-antitoxin genes are responsible for the phenotypic switch to a quasi-dormant state that enables cell survival under stresses,a similar function to heterocyst of cyanobacteria. In this paper,we particularly study the role of gene pair all3211-asl3212 under Spectinomycin stress to reveal how the type Ⅱ toxin-antitoxin involved in environmental stress responses. Bioinformatics prediction shows that toxin protein gene All3211 is homologous to Maz F,a member of maz EF family that encoding nucleases. We clone gene all3211-asl3212 into expression vectors to identify its molecular characteristics. Deletion mutant strains of all3211-asl3212 are selected in a tri-parental mating screen. Phenotype comparisons of mutant and wild type reveals no difference of single-deletion-mutants in pigment integrity,the sensitivity to antibiotics,and heterocyst formation. The results show that deletion mutation of single TAS gene pair all3211-asl3212 results in limited effects on the cellular growth of PCC 7120. Thus,we suggest that dosage compensating might be provided from redundant genes or bypass pathways to offset obvious phenotypic differences.
