为提高复杂气象条件下海上船舶的识别效果,通过暗通道先验去雾算法减少云雾遮挡对目标识别的影响,使用基于深度学习的YOLO(you only look once)改进算法提高目标识别效果。结果表明:采用的算法在中国航天科工四院指挥自动化中心的模拟...为提高复杂气象条件下海上船舶的识别效果,通过暗通道先验去雾算法减少云雾遮挡对目标识别的影响,使用基于深度学习的YOLO(you only look once)改进算法提高目标识别效果。结果表明:采用的算法在中国航天科工四院指挥自动化中心的模拟海事数据集上,4类船舶目标识别的m AP (mean average precision)达到89. 98%,超过了对比的其他目标识别算法;针对数据集中的云雾遮挡图像,暗通道去雾处理后,目标识别的m AP从53. 25%提升到69. 35%。可见提出的算法可以满足复杂气象条件下的海上船舶识别的需求。展开更多
Fusarium wilt, caused by Fusarium oxyporum f. sp. cubense(Foc), is the most serious disease affecting banana production.To clarify the distribution of the Foc races in Fujian Province of China, 79 soil samples were co...Fusarium wilt, caused by Fusarium oxyporum f. sp. cubense(Foc), is the most serious disease affecting banana production.To clarify the distribution of the Foc races in Fujian Province of China, 79 soil samples were collected from four regions of Zhangzhou City, the primary banana production area in Fujian. We isolated and identified 12 Foc strains based on internal transcribed spacer(ITS) sequence analysis, PCR amplification by using Foc-specific primers and pathogenicity assays.Our analysis indicated that 11 isolates belong to Foc race 1, and 1 isolate belongs to the Foc tropical species race 4(TR4).Although TR4 has previously been reported to occur in primary banana-producing provinces, such as Hainan, Guangxi,and Guangdong of China, this is the first report of TR4 isolated from the soil in Fujian Province. Monitoring the presence of Foc, in particular, the TR4 strains in the soil, is the basic strategy to prevent and control Fusarium wilt.展开更多
Background Equatorial lens epithelial cells proliferate and differentiate into fiber cells throughout life, while central lens epithelial cells proliferate little and do not form fiber ceils. This study aimed to inves...Background Equatorial lens epithelial cells proliferate and differentiate into fiber cells throughout life, while central lens epithelial cells proliferate little and do not form fiber ceils. This study aimed to investigate the differences in gene expression between the central and the peripheral epithelial cells of the bovine lens. Methods Lens epithelia were dissected into central （≤11.5 mm diameter, cLEC） and peripheral regions （pLEC）. The differences in gene expression and protein accumulation between these two regions were assayed by microarray analysis and two-dimensional electrophoresis （2-DE） combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. Differently expressed proteins were validated by immunoanalyses. Results By microarray analysis, 67 transcripts were at least two-fold lower and 269 at least two-fold higher in pLEC compared with that in cLEC. Thirty-four protein spots, including 20 in cLEC and 14 in pLEC, were identified by two dimensional electrophoresis and mass spectrometry. Of these 34 protein products, 28 were represented by probe sets on the microarray. Nine transcripts changed in the same direction and four transcripts in the opposite direction to their protein products. Immunoanalyses revealed that three （mitogen-activated protein kinase 1 （MAPK1）, nidogen （NID）, small nuclear ribonucleoprotein N （SNRPN）） out of four transcripts with opposite change between 2-DE and microarray assay showed the same changes as the results of 2-DE gel analyses. The genes differently expressed between cLEC and pLEC mainly include those related to the MAPK, transforming growth factor β （TGFβ） signaling and glycolysis pathways. Conclusion The results suggested that there were distinctly different genome activities, including a specific group of pathways, between central and peripheral lens epithelial cells.展开更多
Background Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis...Background Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis, were performed on the visual cortex during the critical period; few efforts were made to elucidate the molecular changes in the visual cortex during much earlier postnatal stages. The current study aimed to gain a general insight into the molecular mechanisms in the developmental process of the rat visual cortex using microarray to display the gene expression profiles of the visual cortex on postnatal days.Methods All age-matched Sprague-Dawley rats in various groups including postnatal day 0 （PO, n=20）, day 10 （P10,n=15）, day 20 （P20, n=15） and day 45 （P45, n=10） were sacrificed respectively. Fresh visual cortex from the binocular area （Area 17） was dissected for extraction of total RNA for microarray analyses. Taking advantage of annotation information from the gene ontology and pathway database, the gene expression profiles were systematically and globally analyzed.Results Of the 31 042 gene sequences represented on the rat expression microarray, more than 4000 of the transcripts significantly altered at days 45,20 or 10 compared to day 0. The most obvious alteration of gene expression occurred in the first ten days of the postnatal period and the genomic activities of the visual cortex maintained a high level from birth to day 45. Compared to the gene expression at birth, there were 2630 changed transcripts that shared in three postnatal periods.The up-regulated genes in most signaling pathways were more than those of the down-regulated genes.Conclusions Analyzing gene expression patterns, we provide a detailed insight into the molecular organization of the developing visual cortex in the earlier postnatal rat. The most obvious alteration of gene expression in visual cortex occurred in the first ten days. Our data were a basis to identify new relevant candidate genes that control visual cortex development.展开更多
文摘为提高复杂气象条件下海上船舶的识别效果,通过暗通道先验去雾算法减少云雾遮挡对目标识别的影响,使用基于深度学习的YOLO(you only look once)改进算法提高目标识别效果。结果表明:采用的算法在中国航天科工四院指挥自动化中心的模拟海事数据集上,4类船舶目标识别的m AP (mean average precision)达到89. 98%,超过了对比的其他目标识别算法;针对数据集中的云雾遮挡图像,暗通道去雾处理后,目标识别的m AP从53. 25%提升到69. 35%。可见提出的算法可以满足复杂气象条件下的海上船舶识别的需求。
基金supported by the National Natural Science Foundation of China (31601583)the Natural Science Foundation of Fujian Province, China (2016J01113)+1 种基金the Young Teacher Education Research Project of Fujian Province, China (JAT160178)the Fujian Agriculture and Forestry University Outstanding Youth Scientific Research Project, China (xjq201625)
文摘Fusarium wilt, caused by Fusarium oxyporum f. sp. cubense(Foc), is the most serious disease affecting banana production.To clarify the distribution of the Foc races in Fujian Province of China, 79 soil samples were collected from four regions of Zhangzhou City, the primary banana production area in Fujian. We isolated and identified 12 Foc strains based on internal transcribed spacer(ITS) sequence analysis, PCR amplification by using Foc-specific primers and pathogenicity assays.Our analysis indicated that 11 isolates belong to Foc race 1, and 1 isolate belongs to the Foc tropical species race 4(TR4).Although TR4 has previously been reported to occur in primary banana-producing provinces, such as Hainan, Guangxi,and Guangdong of China, this is the first report of TR4 isolated from the soil in Fujian Province. Monitoring the presence of Foc, in particular, the TR4 strains in the soil, is the basic strategy to prevent and control Fusarium wilt.
文摘Background Equatorial lens epithelial cells proliferate and differentiate into fiber cells throughout life, while central lens epithelial cells proliferate little and do not form fiber ceils. This study aimed to investigate the differences in gene expression between the central and the peripheral epithelial cells of the bovine lens. Methods Lens epithelia were dissected into central （≤11.5 mm diameter, cLEC） and peripheral regions （pLEC）. The differences in gene expression and protein accumulation between these two regions were assayed by microarray analysis and two-dimensional electrophoresis （2-DE） combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. Differently expressed proteins were validated by immunoanalyses. Results By microarray analysis, 67 transcripts were at least two-fold lower and 269 at least two-fold higher in pLEC compared with that in cLEC. Thirty-four protein spots, including 20 in cLEC and 14 in pLEC, were identified by two dimensional electrophoresis and mass spectrometry. Of these 34 protein products, 28 were represented by probe sets on the microarray. Nine transcripts changed in the same direction and four transcripts in the opposite direction to their protein products. Immunoanalyses revealed that three （mitogen-activated protein kinase 1 （MAPK1）, nidogen （NID）, small nuclear ribonucleoprotein N （SNRPN）） out of four transcripts with opposite change between 2-DE and microarray assay showed the same changes as the results of 2-DE gel analyses. The genes differently expressed between cLEC and pLEC mainly include those related to the MAPK, transforming growth factor β （TGFβ） signaling and glycolysis pathways. Conclusion The results suggested that there were distinctly different genome activities, including a specific group of pathways, between central and peripheral lens epithelial cells.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 81070765） and the Department of Science ＆ Technology of Guangdong Province, China （No. 2004A30801001）
文摘Background Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis, were performed on the visual cortex during the critical period; few efforts were made to elucidate the molecular changes in the visual cortex during much earlier postnatal stages. The current study aimed to gain a general insight into the molecular mechanisms in the developmental process of the rat visual cortex using microarray to display the gene expression profiles of the visual cortex on postnatal days.Methods All age-matched Sprague-Dawley rats in various groups including postnatal day 0 （PO, n=20）, day 10 （P10,n=15）, day 20 （P20, n=15） and day 45 （P45, n=10） were sacrificed respectively. Fresh visual cortex from the binocular area （Area 17） was dissected for extraction of total RNA for microarray analyses. Taking advantage of annotation information from the gene ontology and pathway database, the gene expression profiles were systematically and globally analyzed.Results Of the 31 042 gene sequences represented on the rat expression microarray, more than 4000 of the transcripts significantly altered at days 45,20 or 10 compared to day 0. The most obvious alteration of gene expression occurred in the first ten days of the postnatal period and the genomic activities of the visual cortex maintained a high level from birth to day 45. Compared to the gene expression at birth, there were 2630 changed transcripts that shared in three postnatal periods.The up-regulated genes in most signaling pathways were more than those of the down-regulated genes.Conclusions Analyzing gene expression patterns, we provide a detailed insight into the molecular organization of the developing visual cortex in the earlier postnatal rat. The most obvious alteration of gene expression in visual cortex occurred in the first ten days. Our data were a basis to identify new relevant candidate genes that control visual cortex development.
