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Determination of the Photoperiod-Sensitive Inductive Phase in Maize with Leaf Numbers and Morphologies of Stem Apical Meristem 被引量:4
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作者 wu lian-cheng WANG Tie-gu +4 位作者 KU Li-xia HUANG Qun-ce SUN Zhao-hui XIA Zhong-liang CHEN Yan-hui 《Agricultural Sciences in China》 CAS CSCD 2008年第5期554-560,共7页
It is vital to determine the effective photoperiods of maize for making full use of tropical germplasm, which is the foundation for determining the effect of latitude and planting date on the development of photoperio... It is vital to determine the effective photoperiods of maize for making full use of tropical germplasm, which is the foundation for determining the effect of latitude and planting date on the development of photoperiod-sensitive maize cultivars. The objective of this study is to determine the photoperiod-sensitive inductive phase using reciprocal transfer between long- day (LD) (15 h d^-1) and short-day conditions (SD) (9 h d^-1). For Huangzao 4 and CML288, days to tassel and pollen shedding were recorded, and stem apical meristems (SAM) were observed by a laser scanning confocal microscope. The results show that the seedlings are insensitive to photoperiod when they are very young (juvenile). However, after this period, LD delays flowering and increases the leaf numbers below the inflorescence, and the length of the interval of the photoperiod-sensitive inductive phase is longer under LD conditions than under SD conditions. Transferred from SD to LD, plants show a sudden decrease in leaf numbers once sufficient SD has been received for flower commitment. While transferred from LD to SD, plants have a continuous increase in leaf numbers during the photoperiod sensitive inductive phase under LD conditions. At the same time, when plants are competent to flowers, the obvious morphology is the elongation of maize SAM. There is an obvious variance of the photoperiod sensitive phase under LD and SD conditions in different maize. 展开更多
关键词 MAIZE PHOTOPERIOD FLOWERING LSCM stem apical meristem (SAM) new full expansion leaf
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阻断授粉诱导玉米叶片提前衰老的转录组分析 被引量:4
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作者 吴连成 李沛 +5 位作者 田磊 王顺喜 李明娜 王宇宇 王赛 陈彦惠 《作物学报》 CAS CSCD 北大核心 2018年第11期1661-1672,共12页
衰老发生在玉米生长发育的最后阶段,叶片适时启动衰老对玉米最终产量的形成具有至关重要的作用。本研究以玉米自交系豫816为试材,采用RNA-seq技术分析阻断授粉诱导玉米叶片提前衰老的分子机制。与正常授粉植株相比,阻断授粉诱导条件下... 衰老发生在玉米生长发育的最后阶段,叶片适时启动衰老对玉米最终产量的形成具有至关重要的作用。本研究以玉米自交系豫816为试材,采用RNA-seq技术分析阻断授粉诱导玉米叶片提前衰老的分子机制。与正常授粉植株相比,阻断授粉诱导条件下的植株在吐丝后27 d,叶片全部变黄并枯萎。吐丝后24 d,授粉植株与同期非授粉植株叶片间的叶绿素含量差异达到极显著水平。差异表达基因(DEG)分析结果显示,吐丝后10d的授粉处理组与同期非授粉处理组比较存在173个DEG;吐丝后24d的授粉处理组与同期非授粉处理组比较存在835个DEG。吐丝后24 d的授粉处理组与吐丝后10 d的授粉处理组比较存在1381个DEG;吐丝后24 d的非授粉处理组与吐丝后10 d的非授粉处理组比较存在1591个DEG。GO功能富集分析发现,吐丝后10 d,授粉处理组和非授粉处理组间DEG的功能主要富集在刺激响应和代谢进程;吐丝后24 d,授粉处理与非授粉处理间DEG的功能主要富集在光合作用进程。Pathway富集分析结果显示,吐丝后10 d,授粉处理与非授粉处理间的DEG主要参与RNA降解、光合作用、木质素合成、转录调控、糖转运代谢路径;吐丝后24 d,授粉处理与非授粉处理间的DEG主要参与信号传导、激素代谢和光合作用路径。阻断授粉诱导处理使豫816植株体内碳水化合物代谢和光合作用受到影响,造成玉米衰老反应的启动和衰老速率显著提前和加快。 展开更多
关键词 玉米 阻断授粉 早衰 转录组
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