AFLP analysis of near-isogenic lines of the stripe rust resistance gene Yr10 was carried out with 6 Pst I -primers and 10 Taq I -primers with the donor parent of Yr1O gene as the check. A total of about 4200 distingui...AFLP analysis of near-isogenic lines of the stripe rust resistance gene Yr10 was carried out with 6 Pst I -primers and 10 Taq I -primers with the donor parent of Yr1O gene as the check. A total of about 4200 distinguishable bands were amplified, of which 5 were stable. The genetic linkage of the 5 polymorphic DNA fragments with the target gene were tested preliminarily on a segregating F2 population derived from a cross between the gene donor parent 'Moro' and susceptible cultivar 'Mingxian 169'. The DNA fragment PT0502 was found closely linked to the Yr10 gene and cloned and sequenced. Based on the sequence specific primers for PCR were designed and synthesized. Genetic linkage analysis with 195 segregating F2 plants indicated that the genetic distance was 0.5 cM between the main product SC200 fragment produced by PCR with the primers and the Yr10 gene. The primers can be used to detect the Yr10 gene quickly, effectively and exactly.展开更多
基金the National "863" Program of China (Grant No. 101-02-01-01).
文摘AFLP analysis of near-isogenic lines of the stripe rust resistance gene Yr10 was carried out with 6 Pst I -primers and 10 Taq I -primers with the donor parent of Yr1O gene as the check. A total of about 4200 distinguishable bands were amplified, of which 5 were stable. The genetic linkage of the 5 polymorphic DNA fragments with the target gene were tested preliminarily on a segregating F2 population derived from a cross between the gene donor parent 'Moro' and susceptible cultivar 'Mingxian 169'. The DNA fragment PT0502 was found closely linked to the Yr10 gene and cloned and sequenced. Based on the sequence specific primers for PCR were designed and synthesized. Genetic linkage analysis with 195 segregating F2 plants indicated that the genetic distance was 0.5 cM between the main product SC200 fragment produced by PCR with the primers and the Yr10 gene. The primers can be used to detect the Yr10 gene quickly, effectively and exactly.
