目的 利用近红外光谱法对羊栖菜生长过程中的活性成分进行快速定量分析,并探究其生长过程中的活性成分含量变化规律。方法 收集羊栖菜7个生长阶段共175个羊栖菜粉末样品,采用紫外-可见分光光度法测定多糖和多酚的含量,高效液相色谱法测...目的 利用近红外光谱法对羊栖菜生长过程中的活性成分进行快速定量分析,并探究其生长过程中的活性成分含量变化规律。方法 收集羊栖菜7个生长阶段共175个羊栖菜粉末样品,采用紫外-可见分光光度法测定多糖和多酚的含量,高效液相色谱法测定岩藻黄质的含量。运用组合区间-偏最小二乘法(synergy interval partial least squares,Si-PLS)分别建立了近红外光谱与3种活性成分指标参考值之间的定量校正模型,并采用不同的预处理方法和主因子数优化模型。结果 在羊栖菜的生长过程中,多糖含量变化为栽培期>快速生长期>成熟期;多酚含量变化为快速生长期>成熟期>栽培期;岩藻黄质含量变化为栽培期>快速生长期≈成熟期。多糖、多酚和岩藻黄质3种定量模型的近红外预测值与参考值之间的拟合性良好,模型预测精度较高,其中预测集相关系数(correlation coefficient of prediction,RP)均大于0.95;预测集均方根误差(root mean square error of prediction,RMSEP)分别为11.01、1.72、0.41mg/g,预测集相对偏差(relative standard of error of prediction, RSEP)分别为8.66%、3.62%、8.67%。结论 本研究揭示了羊栖菜生长过程中多糖、多酚和岩藻黄质的含量变化规律;证实了采用近红外光谱法结合Si-PLS可以成功用于测定羊栖菜生长过程中的活性成分含量,且该方法操作简单、快速、准确、无损、环保。展开更多
Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, whic...Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIPt was determined by using several methods. C.,C analysis indicates that PIP1 is composed of the monosaccharides of glucose, galactose, and mannose. Their malar ratio is 3. 70： 4. 06： 1.00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, pefiedate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP1. The results show that PIP1 has a small quantity of branch structure, The main glycosidic linkage of PIP1 has a β-configurafion. The main chain is made up of a large mass of glucose （ 1→3 ） and few mannose （ 1→4 ） ; the side chain is composed of glucose （ 1 →3 ） and galactose （ 1→6 ） ; the nonreduced end is composed of galactose and glucose. The side chains are branched at 6-0 of glucose（ 1→3,6） and mannose（1→4,6）. On an average, there are three branches among 20 residues. It is presumable that the existence of 1,3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.展开更多
文摘目的 利用近红外光谱法对羊栖菜生长过程中的活性成分进行快速定量分析,并探究其生长过程中的活性成分含量变化规律。方法 收集羊栖菜7个生长阶段共175个羊栖菜粉末样品,采用紫外-可见分光光度法测定多糖和多酚的含量,高效液相色谱法测定岩藻黄质的含量。运用组合区间-偏最小二乘法(synergy interval partial least squares,Si-PLS)分别建立了近红外光谱与3种活性成分指标参考值之间的定量校正模型,并采用不同的预处理方法和主因子数优化模型。结果 在羊栖菜的生长过程中,多糖含量变化为栽培期>快速生长期>成熟期;多酚含量变化为快速生长期>成熟期>栽培期;岩藻黄质含量变化为栽培期>快速生长期≈成熟期。多糖、多酚和岩藻黄质3种定量模型的近红外预测值与参考值之间的拟合性良好,模型预测精度较高,其中预测集相关系数(correlation coefficient of prediction,RP)均大于0.95;预测集均方根误差(root mean square error of prediction,RMSEP)分别为11.01、1.72、0.41mg/g,预测集相对偏差(relative standard of error of prediction, RSEP)分别为8.66%、3.62%、8.67%。结论 本研究揭示了羊栖菜生长过程中多糖、多酚和岩藻黄质的含量变化规律;证实了采用近红外光谱法结合Si-PLS可以成功用于测定羊栖菜生长过程中的活性成分含量,且该方法操作简单、快速、准确、无损、环保。
基金Supported by the Science and Technique Bureau of Wenzhou City(No. S2005A003).
文摘Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIPt was determined by using several methods. C.,C analysis indicates that PIP1 is composed of the monosaccharides of glucose, galactose, and mannose. Their malar ratio is 3. 70： 4. 06： 1.00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, pefiedate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP1. The results show that PIP1 has a small quantity of branch structure, The main glycosidic linkage of PIP1 has a β-configurafion. The main chain is made up of a large mass of glucose （ 1→3 ） and few mannose （ 1→4 ） ; the side chain is composed of glucose （ 1 →3 ） and galactose （ 1→6 ） ; the nonreduced end is composed of galactose and glucose. The side chains are branched at 6-0 of glucose（ 1→3,6） and mannose（1→4,6）. On an average, there are three branches among 20 residues. It is presumable that the existence of 1,3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.
