为探讨土壤增氮对Bt棉棉铃对位叶杀虫蛋白表达量影响程度及相关的氮代谢生理机制,提高Bt棉花铃期抗虫性的农学调节提供理论和技术支撑。采用裂区设计,以Bt棉常规品种泗抗1号(SK-1)、杂交品种泗抗3号(SK-3)为材料,在常规施氮量300kghm^(...为探讨土壤增氮对Bt棉棉铃对位叶杀虫蛋白表达量影响程度及相关的氮代谢生理机制,提高Bt棉花铃期抗虫性的农学调节提供理论和技术支撑。采用裂区设计,以Bt棉常规品种泗抗1号(SK-1)、杂交品种泗抗3号(SK-3)为材料,在常规施氮量300kghm^(-2)基础上,设计施氮量分别增加25%、50%、75%、100%的处理,研究土壤增氮对Bt棉棉铃对位叶杀虫蛋白表达量影响。结果表明,2个类型品种棉铃对位叶中Bt杀虫蛋白含量均随增施氮量提高呈一直增加的特征,与对照相比,施氮量增加25%~100%,棉铃对位叶Bt杀虫蛋白增加6.1%~96.9%。氮代谢生理机制进一步表明,棉铃对位叶中可溶性蛋白含量、游离氨基酸含量、谷氨酸丙酮酸转氨酶活性和谷氨酰胺合成酶活性的变化趋势与Bt蛋白含量表现一致,而蛋白质分解关键酶(蛋白酶、肽酶)活性则随施氮量的增加呈下降趋势。叶面积指数(LAI)随增施氮量的提高而增大,而产量则随土壤氮量的增加呈先升高后降低特征,最适LAI和产量最大值均出现在常规施氮的1.25倍(375 kg hm^(-2))。综上,在常规施氮基础上适量增施氮肥有利于棉铃对位叶中Bt蛋白的合成、最适LAI的形成和产量的提高,有利于实现Bt棉丰产性和抗虫性协同表达。展开更多
The paper is to explore the English Metaphysical ideas reflected in Anne bradstreet's first poetry The Tenth Muse Lately Sprung Up in America. The whole paper is devided into three parts , the first part is to int...The paper is to explore the English Metaphysical ideas reflected in Anne bradstreet's first poetry The Tenth Muse Lately Sprung Up in America. The whole paper is devided into three parts , the first part is to introduce English Metaphysical ideas and Anne's first and only published poetry before death,The second one is to analyze the reflection of the English Metaphysical ideas in Anne's first poetry in terms of the themes, the images and the rhetorical devices of the poetry. the last one is the conclusion of the essay .展开更多
文摘为探讨土壤增氮对Bt棉棉铃对位叶杀虫蛋白表达量影响程度及相关的氮代谢生理机制,提高Bt棉花铃期抗虫性的农学调节提供理论和技术支撑。采用裂区设计,以Bt棉常规品种泗抗1号(SK-1)、杂交品种泗抗3号(SK-3)为材料,在常规施氮量300kghm^(-2)基础上,设计施氮量分别增加25%、50%、75%、100%的处理,研究土壤增氮对Bt棉棉铃对位叶杀虫蛋白表达量影响。结果表明,2个类型品种棉铃对位叶中Bt杀虫蛋白含量均随增施氮量提高呈一直增加的特征,与对照相比,施氮量增加25%~100%,棉铃对位叶Bt杀虫蛋白增加6.1%~96.9%。氮代谢生理机制进一步表明,棉铃对位叶中可溶性蛋白含量、游离氨基酸含量、谷氨酸丙酮酸转氨酶活性和谷氨酰胺合成酶活性的变化趋势与Bt蛋白含量表现一致,而蛋白质分解关键酶(蛋白酶、肽酶)活性则随施氮量的增加呈下降趋势。叶面积指数(LAI)随增施氮量的提高而增大,而产量则随土壤氮量的增加呈先升高后降低特征,最适LAI和产量最大值均出现在常规施氮的1.25倍(375 kg hm^(-2))。综上,在常规施氮基础上适量增施氮肥有利于棉铃对位叶中Bt蛋白的合成、最适LAI的形成和产量的提高,有利于实现Bt棉丰产性和抗虫性协同表达。
文摘The paper is to explore the English Metaphysical ideas reflected in Anne bradstreet's first poetry The Tenth Muse Lately Sprung Up in America. The whole paper is devided into three parts , the first part is to introduce English Metaphysical ideas and Anne's first and only published poetry before death,The second one is to analyze the reflection of the English Metaphysical ideas in Anne's first poetry in terms of the themes, the images and the rhetorical devices of the poetry. the last one is the conclusion of the essay .
