目的运用基因集富集分析(GSEA)探索人表皮生长因子受体2(HER2)基因表达状态对胃癌代谢通路影响情况。方法下载癌症基因组图谱(TCGA)、基因表达综合数据库(GEO)和欧洲生物信息研究所(ArrayExpress)数据库的胃癌转录表达数据库,根据Her2...目的运用基因集富集分析(GSEA)探索人表皮生长因子受体2(HER2)基因表达状态对胃癌代谢通路影响情况。方法下载癌症基因组图谱(TCGA)、基因表达综合数据库(GEO)和欧洲生物信息研究所(ArrayExpress)数据库的胃癌转录表达数据库,根据Her2拷贝数或者表达水平选取高低表达组,应用GSEA软件进行富集分析,取错误发现率q值(FDR q val)<25%和/或名义P值(nom P val)<0.01的代谢通路作为有意义的代谢基因集,结果绘制热图寻找共性。结果在癌症基因组图谱胃腺癌集(TCGA STAD)、GEO数据集(GSE66229)等10个数据库中,Her2的高低表达对对过氧物酶体、N-聚糖生物合成和嘧啶代谢通路基因集有影响(FDR q val<25%且nom P val<0.01),糖基磷脂酰肌醇、甘油磷脂、鞘脂类代谢差异有统计学意义(nom P val<0.01)。结论多个数据库GSEA分析结果提示癌基因Her2的状态与多个代谢通路基因集有相关性。展开更多
In order to comprehensively and objectively understand the research status of street form at home and abroad,WOS and CNKI database are as the source,and 176 street form papers retrieved from 2010 to 2021 are as sample...In order to comprehensively and objectively understand the research status of street form at home and abroad,WOS and CNKI database are as the source,and 176 street form papers retrieved from 2010 to 2021 are as samples to conduct statistical analysis of the data.The main research hotspots are reviewed systematically,and the research trends in the field of planning and architecture are pointed out.The hot topics of street form research in the past ten years are summarized,such as street spatial form characteristics,street form and thermal environment,air quality correlation,street form and walking,safety,vitality,health and other perceived correlations.The research methods are summarized from two levels of street form data collection and analysis.Finally,the current research trend characteristics and future trends are summarized and prospected to provide reference for the current research of street form.展开更多
文摘目的运用基因集富集分析(GSEA)探索人表皮生长因子受体2(HER2)基因表达状态对胃癌代谢通路影响情况。方法下载癌症基因组图谱(TCGA)、基因表达综合数据库(GEO)和欧洲生物信息研究所(ArrayExpress)数据库的胃癌转录表达数据库,根据Her2拷贝数或者表达水平选取高低表达组,应用GSEA软件进行富集分析,取错误发现率q值(FDR q val)<25%和/或名义P值(nom P val)<0.01的代谢通路作为有意义的代谢基因集,结果绘制热图寻找共性。结果在癌症基因组图谱胃腺癌集(TCGA STAD)、GEO数据集(GSE66229)等10个数据库中,Her2的高低表达对对过氧物酶体、N-聚糖生物合成和嘧啶代谢通路基因集有影响(FDR q val<25%且nom P val<0.01),糖基磷脂酰肌醇、甘油磷脂、鞘脂类代谢差异有统计学意义(nom P val<0.01)。结论多个数据库GSEA分析结果提示癌基因Her2的状态与多个代谢通路基因集有相关性。
基金Sponsored by the Key Project of Humanities and Social Sciences in Higher Education Institutions in Hebei Province(SD201075)the Research and Practice Project of Higher Education Teaching Reform in Hebei Province(2019GJJG249)the Construction Project of Postgraduate Demonstration Courses in Hebei Province(KCJS2019069)。
文摘In order to comprehensively and objectively understand the research status of street form at home and abroad,WOS and CNKI database are as the source,and 176 street form papers retrieved from 2010 to 2021 are as samples to conduct statistical analysis of the data.The main research hotspots are reviewed systematically,and the research trends in the field of planning and architecture are pointed out.The hot topics of street form research in the past ten years are summarized,such as street spatial form characteristics,street form and thermal environment,air quality correlation,street form and walking,safety,vitality,health and other perceived correlations.The research methods are summarized from two levels of street form data collection and analysis.Finally,the current research trend characteristics and future trends are summarized and prospected to provide reference for the current research of street form.
