Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.V...Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.Visualizing murine liver microvasculature ex vivo with phase-contrast X-ray imaging was performed at Shanghai Synchrotron Radiation Facility.Barium sulfate and physiological saline were used as contrast agents for the blood vessels.Blood vessels of <Φ20μm could be detected by replacing resident blood with physiological saline or barium sulfate.An entire branch of the portal vein (from the main axial portal vein to the ninth generation of branching) could be captured in a single phase-contrast image.It is demonstrated that selective angiography based on phase contrast X-ray imaging,with a physiological material of low Z elements (such as saline) being the contrast agent,is a viable imaging strategy.Further efforts will be focused on using the technique to image tumor angiogenesis.展开更多
针对污水处理厂深度脱氮需求,选用黄铁矿(FeS_(2))和聚羟基丁酸戊酸酯(PHBV)两种材料,构建应用于污水处理厂二级出水深度脱氮的反硝化生物填充床,通过进出水水质监测、材料结构表征(扫描电镜)、高通量测序等技术手段,探究了FeS_(2)-PHB...针对污水处理厂深度脱氮需求,选用黄铁矿(FeS_(2))和聚羟基丁酸戊酸酯(PHBV)两种材料,构建应用于污水处理厂二级出水深度脱氮的反硝化生物填充床,通过进出水水质监测、材料结构表征(扫描电镜)、高通量测序等技术手段,探究了FeS_(2)-PHBV协同自养-异养反硝化体系的工艺运行效果及机理.结果表明,FeS_(2)-PHBV协同反硝化体系脱氮速率优于单独FeS_(2)自养体系,当水力停留时间为2h时,脱氮效率为90%以上,且产生更少的硫酸盐副产物.相比于单独PHBV异养体系,协同体系在达到相同总氮去除效果的同时释放更低的DOC(23.62mg/L).FeS_(2)-PHBV协同体系中两种材料表面结构均较利用前发生了显著变化,这是由于材料表面生物膜的富集和利用.共混反应器内优势菌群为厌氧发酵菌属(Sporomusaceae)和梭菌属(Clostridium sensu stricto 7),两者实现了共混体系异养-自养的协同反硝化效果,其中Fe代谢相关梭菌属(Clostridium)主导的自养反硝化在共混系统脱氮过程中可能发挥着重要作用.展开更多
OBJECTIVE:To investigate the efficacy of an herbal formula of Bushen Jianpi(补肾健脾方,BSJP)combined with sorafenib on hepatocellular carcinoma(HCC)in vitro and in vivo,and to study the underlying mechanisms of action...OBJECTIVE:To investigate the efficacy of an herbal formula of Bushen Jianpi(补肾健脾方,BSJP)combined with sorafenib on hepatocellular carcinoma(HCC)in vitro and in vivo,and to study the underlying mechanisms of action.METHODS:BSJP,a mixture of 12 raw herbs,was extracted in 70%alcohol/30%water and freeze-dried into a powder.The in vitro effects of BSJP alone,sorafenib alone,and their combination on cell survival,apoptosis,and cell cycle distribution were evaluated in HCC cell lines HCCLM3,HepG2,and SMMC-7721.The expression of B-cell lymphoma-2(Bcl-2),caspase-3,and caspase-9 in HCCLM3 cells was measured using Western blots after drug administration.The in vivo effects of BSJP and sorafenib were evaluated in a tumor surgical resection model using 4-week old male athymic BALB/c nude mice injected with HCCLM3 cells.Immunohistochemical analysis of tumor tissues was performed to evaluate the effects of BSJP alone,sorafenib alone,and their combination on the expression of caspase-3,caspase-9,and Bcl-2.RESULTS:BSJP decreased the survival rate of HCC cell lines,and the combination of BSJP and sorafenib further decreased the survival rate.BSJP significantly promoted cell apoptosis and blocked cell-cycle progression in HCCLM3,HepG2,and SMMC-7721 cells in a dose-dependent manner.Furthermore,the administration of BSJP and sorafenib inhibited the growth of HCCLM3 cell xenografts in nude mice,with no reduction in body weight.In vivo and in vitro experiments showed that BSJP combined with sorafenib could significantly decrease the expression of Bcl-2.CONCLUSION:Our findings suggest that the herbal formula of BSJP is a potential HCC antitumor agent.展开更多
基金Supported by National Basic Research Program of China (973 Program Grant No.2010CB834305)
文摘Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.Visualizing murine liver microvasculature ex vivo with phase-contrast X-ray imaging was performed at Shanghai Synchrotron Radiation Facility.Barium sulfate and physiological saline were used as contrast agents for the blood vessels.Blood vessels of <Φ20μm could be detected by replacing resident blood with physiological saline or barium sulfate.An entire branch of the portal vein (from the main axial portal vein to the ninth generation of branching) could be captured in a single phase-contrast image.It is demonstrated that selective angiography based on phase contrast X-ray imaging,with a physiological material of low Z elements (such as saline) being the contrast agent,is a viable imaging strategy.Further efforts will be focused on using the technique to image tumor angiogenesis.
文摘针对污水处理厂深度脱氮需求,选用黄铁矿(FeS_(2))和聚羟基丁酸戊酸酯(PHBV)两种材料,构建应用于污水处理厂二级出水深度脱氮的反硝化生物填充床,通过进出水水质监测、材料结构表征(扫描电镜)、高通量测序等技术手段,探究了FeS_(2)-PHBV协同自养-异养反硝化体系的工艺运行效果及机理.结果表明,FeS_(2)-PHBV协同反硝化体系脱氮速率优于单独FeS_(2)自养体系,当水力停留时间为2h时,脱氮效率为90%以上,且产生更少的硫酸盐副产物.相比于单独PHBV异养体系,协同体系在达到相同总氮去除效果的同时释放更低的DOC(23.62mg/L).FeS_(2)-PHBV协同体系中两种材料表面结构均较利用前发生了显著变化,这是由于材料表面生物膜的富集和利用.共混反应器内优势菌群为厌氧发酵菌属(Sporomusaceae)和梭菌属(Clostridium sensu stricto 7),两者实现了共混体系异养-自养的协同反硝化效果,其中Fe代谢相关梭菌属(Clostridium)主导的自养反硝化在共混系统脱氮过程中可能发挥着重要作用.
基金Shanghai Science and Technology Commission:a Randomized,Controlled,Double-blind Clinical Study of Xianglian Pill on Immunotherapy of Advanced Malignant Tumor(No.19401971600)Hongkou District Health Committee National Medicine:Traditional Chinese Medicine Oncology Specialty and Comprehensive Treatment Area Project(No.HGY-ZHZL-2018-03)Budget Project of Shanghai University of Traditional Chinese Medicine:Exploring the Mechanism of Berberine Reversing T Cell Failure and Enhancing the Curative Effect of Immunotherapy for Lung Cancer Based on Tox Gene(2020TS101)。
文摘OBJECTIVE:To investigate the efficacy of an herbal formula of Bushen Jianpi(补肾健脾方,BSJP)combined with sorafenib on hepatocellular carcinoma(HCC)in vitro and in vivo,and to study the underlying mechanisms of action.METHODS:BSJP,a mixture of 12 raw herbs,was extracted in 70%alcohol/30%water and freeze-dried into a powder.The in vitro effects of BSJP alone,sorafenib alone,and their combination on cell survival,apoptosis,and cell cycle distribution were evaluated in HCC cell lines HCCLM3,HepG2,and SMMC-7721.The expression of B-cell lymphoma-2(Bcl-2),caspase-3,and caspase-9 in HCCLM3 cells was measured using Western blots after drug administration.The in vivo effects of BSJP and sorafenib were evaluated in a tumor surgical resection model using 4-week old male athymic BALB/c nude mice injected with HCCLM3 cells.Immunohistochemical analysis of tumor tissues was performed to evaluate the effects of BSJP alone,sorafenib alone,and their combination on the expression of caspase-3,caspase-9,and Bcl-2.RESULTS:BSJP decreased the survival rate of HCC cell lines,and the combination of BSJP and sorafenib further decreased the survival rate.BSJP significantly promoted cell apoptosis and blocked cell-cycle progression in HCCLM3,HepG2,and SMMC-7721 cells in a dose-dependent manner.Furthermore,the administration of BSJP and sorafenib inhibited the growth of HCCLM3 cell xenografts in nude mice,with no reduction in body weight.In vivo and in vitro experiments showed that BSJP combined with sorafenib could significantly decrease the expression of Bcl-2.CONCLUSION:Our findings suggest that the herbal formula of BSJP is a potential HCC antitumor agent.
