In this study,FTIR and XPS spectroscopy,specific surface area and pore size analyses,wetting and other experimental means have been applied to investigate respirable coal dust surface properties and wetting mechanism....In this study,FTIR and XPS spectroscopy,specific surface area and pore size analyses,wetting and other experimental means have been applied to investigate respirable coal dust surface properties and wetting mechanism.The research shows that coal dust surface structures,chemical and element compositions are changed in the refining process of coal dust.Compared with large particle coal dust,respirable coal dust is a weak hydrophilic and strong hydrophobic substance.The reason:the smaller the particle size of coal dust,the more unsaturated its surface.The absorption of air is strong and it is easy to form an air film on coal dust surfaces. Coal dust contact with water is actually a compound contact of liquid in contact with a solid and gas in contact with a solid. The smaller the particle size,the larger the pore volume and the higher the degree of surface roughness,the larger the area of gas in contact with a solid and the worse the coal dust wettability.Another reason is that the changes in the composition of atoms on the dust surface and structures affect coal dust wettability.The results of this study establish a theoretical basis for the invention of effective reduced-dust technology.展开更多
在气候变暖及人类活动的双重干扰下,疏勒河上游冻土发生了显著退化,如活动层厚度加大、植被退化等,而冻土退化对微生物的影响一直是科研人员关注的热点话题。以疏勒河上游不同季节(4月、6月、9月)、不同退化程度冻土为研究对象,研究了...在气候变暖及人类活动的双重干扰下,疏勒河上游冻土发生了显著退化,如活动层厚度加大、植被退化等,而冻土退化对微生物的影响一直是科研人员关注的热点话题。以疏勒河上游不同季节(4月、6月、9月)、不同退化程度冻土为研究对象,研究了可培养细菌多样性特征。通过16S r DNA基因测序及构建系统发育树表明,研究区域可培养细菌归类为27个属,分属于α-变形菌门,γ-变形菌门,放线菌门,厚壁菌门和拟杆菌门,其中放线菌门为优势类群。从属水平来讲,可培养细菌以节杆菌属和微球菌属为主,其含量随冻土退化程度加深分别呈下降和升高趋势。土壤细菌多样性与环境因子的相关性分析表明,可培养细菌多样性与土壤含水量、总氮极显著正相关,与有机碳显著正相关。这些结果表明,伴随着冻土退化而发生的地上植被逆向演替过程中,青藏高原不同类型冻土间已产生较大的环境异质性如土壤碳氮及含水量,进一步可能导致冻土微生物多样性分异。研究结果为利用微生物综合评价青藏高原不同类型冻土的生态环境提供了数据基础。展开更多
Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon S...Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs (Operational Taxonomic Units) in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacterwere the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs (including OTU3615, OTU6535, and OTU6646) between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and pH values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems.展开更多
基金Financial support for this work,provided by the Science and Technology Project of the Education Department of Shandong Province(No.J08LC12),
文摘In this study,FTIR and XPS spectroscopy,specific surface area and pore size analyses,wetting and other experimental means have been applied to investigate respirable coal dust surface properties and wetting mechanism.The research shows that coal dust surface structures,chemical and element compositions are changed in the refining process of coal dust.Compared with large particle coal dust,respirable coal dust is a weak hydrophilic and strong hydrophobic substance.The reason:the smaller the particle size of coal dust,the more unsaturated its surface.The absorption of air is strong and it is easy to form an air film on coal dust surfaces. Coal dust contact with water is actually a compound contact of liquid in contact with a solid and gas in contact with a solid. The smaller the particle size,the larger the pore volume and the higher the degree of surface roughness,the larger the area of gas in contact with a solid and the worse the coal dust wettability.Another reason is that the changes in the composition of atoms on the dust surface and structures affect coal dust wettability.The results of this study establish a theoretical basis for the invention of effective reduced-dust technology.
文摘在气候变暖及人类活动的双重干扰下,疏勒河上游冻土发生了显著退化,如活动层厚度加大、植被退化等,而冻土退化对微生物的影响一直是科研人员关注的热点话题。以疏勒河上游不同季节(4月、6月、9月)、不同退化程度冻土为研究对象,研究了可培养细菌多样性特征。通过16S r DNA基因测序及构建系统发育树表明,研究区域可培养细菌归类为27个属,分属于α-变形菌门,γ-变形菌门,放线菌门,厚壁菌门和拟杆菌门,其中放线菌门为优势类群。从属水平来讲,可培养细菌以节杆菌属和微球菌属为主,其含量随冻土退化程度加深分别呈下降和升高趋势。土壤细菌多样性与环境因子的相关性分析表明,可培养细菌多样性与土壤含水量、总氮极显著正相关,与有机碳显著正相关。这些结果表明,伴随着冻土退化而发生的地上植被逆向演替过程中,青藏高原不同类型冻土间已产生较大的环境异质性如土壤碳氮及含水量,进一步可能导致冻土微生物多样性分异。研究结果为利用微生物综合评价青藏高原不同类型冻土的生态环境提供了数据基础。
基金supported by the National Natural Science Foundation of China(41271265,31570498,31300411)the Science and Technology Projects in Gansu Province(1304NKCA135)the Foundation of China Scholarship Council
文摘Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs (Operational Taxonomic Units) in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacterwere the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs (including OTU3615, OTU6535, and OTU6646) between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and pH values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems.