OBJECTIVE:To investigate the effects of Aspongopus(A.)chinensis hemolymph on the proliferation and metastasis of breast cancer cells.METHODS:The in vitro effects of A.chinensis hemolymph were investigated in murine(4T...OBJECTIVE:To investigate the effects of Aspongopus(A.)chinensis hemolymph on the proliferation and metastasis of breast cancer cells.METHODS:The in vitro effects of A.chinensis hemolymph were investigated in murine(4T1)and human(HCC1937)breast cancer cell lines.Cytotoxicity,cell apoptosis,and cell migration were evaluated by using the cell counting kit-8 assay,Hoechst staining,and wound healing experiments,respectively.A syngeneic mouse model was established to evaluate the in vivo effects of the hemolymph extract on tumor growth and metastasis.Mouse body weight,tumor size,blood levels of function-related enzymes,and pathological features of the liver and kidney tissues were evaluated.RESULTS:The hemolymph of A.chinensis significantly inhibited in vitro tumor cell migration and viability while inducing apoptosis.Furthermore,it inhibited in vivo tumor growth and metastasis with a minimal effect on mouse body weight,and did not induce liver or kidney damage.CONCLUSION:Our results suggested that the A.chinensis hemolymph has antitumorigenic properties,suggesting it has potential as a novel therapeutic option for the treatment of metastatic breast cancer.展开更多
基金Supported by grants from the National Nature Science Foundation of China:Isolation and Identification of Anti-gastric-cancer components from Aspongopus chinensis Dallas with its Mechanism(NSFC 81360612)Inhibitory Effect of lentivirus-mediated Cyt-c Gene from Aspongopus chinensis Dallas on Breast Cancer Cell in vitro and in vivo(NSFC81803968)Graduate Education Innovation Project of Guizhou Province:Virus-like Particles Mediated FGF1-P2A-CYCS Fusion Protein induced Transmembrane Transport and Target Cancer Cells Apoptosis(Qian Jiao He YJSCXJH,No.(2019)025)。
文摘OBJECTIVE:To investigate the effects of Aspongopus(A.)chinensis hemolymph on the proliferation and metastasis of breast cancer cells.METHODS:The in vitro effects of A.chinensis hemolymph were investigated in murine(4T1)and human(HCC1937)breast cancer cell lines.Cytotoxicity,cell apoptosis,and cell migration were evaluated by using the cell counting kit-8 assay,Hoechst staining,and wound healing experiments,respectively.A syngeneic mouse model was established to evaluate the in vivo effects of the hemolymph extract on tumor growth and metastasis.Mouse body weight,tumor size,blood levels of function-related enzymes,and pathological features of the liver and kidney tissues were evaluated.RESULTS:The hemolymph of A.chinensis significantly inhibited in vitro tumor cell migration and viability while inducing apoptosis.Furthermore,it inhibited in vivo tumor growth and metastasis with a minimal effect on mouse body weight,and did not induce liver or kidney damage.CONCLUSION:Our results suggested that the A.chinensis hemolymph has antitumorigenic properties,suggesting it has potential as a novel therapeutic option for the treatment of metastatic breast cancer.
