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A drainage-enhancing device for foam fractionation of proteins 被引量:8
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作者 wu zhaoliang qian shaoyu zheng huijie zhao yanli 《Chinese Science Bulletin》 SCIE EI CAS 2010年第12期1213-1220,共8页
The foam fractionation of nisin from its fermentation broth was studied.Two types of devices consisting of a rubber piston and a foam riser were developed to enhance foam drainage.The separation performance of these t... The foam fractionation of nisin from its fermentation broth was studied.Two types of devices consisting of a rubber piston and a foam riser were developed to enhance foam drainage.The separation performance of these two devices was investigated.Experimental results indicated that the second device could significantly reduce the liquid fraction of the foam leaving the column,εout,leading to a higher enrichment of the out-flow stream.As its mounting height increased from 0 to 15 cm,εout declined from 7.07‰ to 6.13 ‰ and the maximum nisin activity in the foamate could reach 39.6 IU/μL.The slight increase in nisin inactivation rate indicated the applicability of this method in the recovery and concentration of proteins.Finally,the mechanism of the process was primarily explained by invoking recent work on pneumatic foams.This research provides a basis for the design of multistage draining foam fractionator which could potentially be an effective separation equipment. 展开更多
关键词 泡沫分离 蛋白质浓度 泡沫排水 分离设备 乳酸链球菌素 NISIN 橡胶活塞 分离性能
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