目的观察标准方位眼底摄像技术在早产儿视网膜病变(retinopathy of prematurity,ROP)远程筛查中的应用价值。方法将2016年11月至2018年2月通过我院ROP远程筛查阅片系统筛查的早产儿1402例2804眼纳入研究,男711例1422眼,女691例1382眼。...目的观察标准方位眼底摄像技术在早产儿视网膜病变(retinopathy of prematurity,ROP)远程筛查中的应用价值。方法将2016年11月至2018年2月通过我院ROP远程筛查阅片系统筛查的早产儿1402例2804眼纳入研究,男711例1422眼,女691例1382眼。所有早产儿均使用双目间接眼底镜检查和广角数码视网膜成像系统完成眼底标准方位摄像,并将图像上传至远程阅片系统进行远程阅片。统计远程阅片系统检出ROP发病的阳性率、假阴性率、灵敏度、特异度以及ROP总发病率,评价标准方位眼底摄像技术在ROP远程筛查中的价值。结果1402例2804眼经远程筛查系统阅片,84例168眼发病,阳性率为6.0%;因图像模糊或方位不佳导致阅片困难,可疑病变,而双目间接眼底镜检查确诊的ROP患者5例10眼,假阴性率为0.3%,灵敏度为94.4%;所有患儿总发病率为6.3%,需要激光或抗血管内皮生长因子(vascular endothelial growth factor,VEGF)治疗的重症ROP患儿28例56眼,占2.0%,其中急进型后极部ROP患儿5例10眼、阈值期患儿14例28眼、阈值前1型患儿9例18眼。重症ROP患儿中,26例52眼经一次激光或抗VEGF治疗后病情得到控制,2例4眼急进型后极部ROP患儿经抗VEGF治疗后4周病情复发,再次给予激光光凝治疗后病情稳定。所有患儿无一例漏诊、误诊、贻误最佳治疗时机者。结论标准方位眼底摄像技术是ROP远程筛查的有力保障;ROP远程筛查阅片系统对ROP病变检出灵敏度高,可以有效解决目前ROP专业防治人员相对缺乏的困境。展开更多
Analysis of phylogenetic and evolution in six species of Sorghum was based on internal transcribed spacer （ITS） sequences in nuclear ribosomal DNA. Results showed that the length of the ITS regions among the six spe...Analysis of phylogenetic and evolution in six species of Sorghum was based on internal transcribed spacer （ITS） sequences in nuclear ribosomal DNA. Results showed that the length of the ITS regions among the six species ranged from 588 to 589 bp and the contents of G＋C in ITS （ITS1 ＋5.8S＋ITS2） regions ranged from 60.27 to 61.05%; the length of ITS1 ranged from 207 to 208 bp and the contents of G ＋ C in the ITS 1 regions ranged from 53.91 to 61.54%. The length of the 5.8S rDNA and ITS2 regions in the six species was 164 and 217 bp respectively, and the contents of G ＋ C ranged from 56.10 to 58.54% in the 5.8S rDNA region and 66.36 to 67,28% in the ITS2 region. Among regions of ITS, ITS1, ITS2, and 5.8S, the best sequence for genetic relationship analysis in the six species was the ITS region. On the basis of the Jaccard coefficient and phylogentic tree, S. sp. was more related to S, propinguum than to other species. This was consistent with the fact that S. sp. is derived from S. propinguum. From the phylogenetic tree based on ITS1, S. halepense, silk sorghum and S. sudanense, are identical in the ITS 1 sequence, whereas the phylogenetic tree based one shows that S. sudanense has a closer genetic association with S. almum rather than with S. halepense and silk sorghum.展开更多
文摘Analysis of phylogenetic and evolution in six species of Sorghum was based on internal transcribed spacer （ITS） sequences in nuclear ribosomal DNA. Results showed that the length of the ITS regions among the six species ranged from 588 to 589 bp and the contents of G＋C in ITS （ITS1 ＋5.8S＋ITS2） regions ranged from 60.27 to 61.05%; the length of ITS1 ranged from 207 to 208 bp and the contents of G ＋ C in the ITS 1 regions ranged from 53.91 to 61.54%. The length of the 5.8S rDNA and ITS2 regions in the six species was 164 and 217 bp respectively, and the contents of G ＋ C ranged from 56.10 to 58.54% in the 5.8S rDNA region and 66.36 to 67,28% in the ITS2 region. Among regions of ITS, ITS1, ITS2, and 5.8S, the best sequence for genetic relationship analysis in the six species was the ITS region. On the basis of the Jaccard coefficient and phylogentic tree, S. sp. was more related to S, propinguum than to other species. This was consistent with the fact that S. sp. is derived from S. propinguum. From the phylogenetic tree based on ITS1, S. halepense, silk sorghum and S. sudanense, are identical in the ITS 1 sequence, whereas the phylogenetic tree based one shows that S. sudanense has a closer genetic association with S. almum rather than with S. halepense and silk sorghum.
