BASED on the hypothesis of Pauling on enzyme action that free energy difference between theground and transition state is reduced by the specific binding of active center of enzyme to theintermediate,a large number of...BASED on the hypothesis of Pauling on enzyme action that free energy difference between theground and transition state is reduced by the specific binding of active center of enzyme to theintermediate,a large number of antibodies with catalytic power have been obtained successive-展开更多
WE have applied the differential display (D. D.) of mRNA method to detect and clone tran-scripts that were differentially expressed between rice NILs (Near-isogenic lines) H7R (resis-tant) and H7S (susceptible) which ...WE have applied the differential display (D. D.) of mRNA method to detect and clone tran-scripts that were differentially expressed between rice NILs (Near-isogenic lines) H7R (resis-tant) and H7S (susceptible) which were induced by Magnaporthe grisea. The cDNA gene-tags obtained from D. D. analysis were reamplified by PCR and screened by Southern展开更多
文摘BASED on the hypothesis of Pauling on enzyme action that free energy difference between theground and transition state is reduced by the specific binding of active center of enzyme to theintermediate,a large number of antibodies with catalytic power have been obtained successive-
文摘WE have applied the differential display (D. D.) of mRNA method to detect and clone tran-scripts that were differentially expressed between rice NILs (Near-isogenic lines) H7R (resis-tant) and H7S (susceptible) which were induced by Magnaporthe grisea. The cDNA gene-tags obtained from D. D. analysis were reamplified by PCR and screened by Southern
