A HPLC Assay was developed to identify and measure the metabolite of acrylamide, mercapturic acid, N-Acetyl-s-(propionamide)-cysteine (APC) in urine. O-phthalaldehyde (OPA) was utilized as a precolumn derivatizing age...A HPLC Assay was developed to identify and measure the metabolite of acrylamide, mercapturic acid, N-Acetyl-s-(propionamide)-cysteine (APC) in urine. O-phthalaldehyde (OPA) was utilized as a precolumn derivatizing agent in the assay. This derivative was isolated with a good selectivity by high performance liquid chromatography (HPLC) employing reversed phase ODS columns. The quantitation of the mercapturic acid derivative was reproducible and with a detection limit of 1 pmol. The average coefficient of variation for the runs carried out on the same day was approximately 4.6% at the range of 80-160 ianol-L'1 of APC, and the mean analytical recovery from urine samples was 94%.The metabolite of urine of workers exposed to acrylamide was identified as N-acetyl-s-(propionamide)- cysteine by HPLC chromatography and fluorescence scan and HPLC-Mass spectra. All results were identical with the authentic synthesized compound.展开更多
A gas chromatographic method for the determination of deltamethrin and fenvalerate in human urine is described. Both deltamethrin and fenvalerate have been analysed by gas chromatography with a electron capture detect...A gas chromatographic method for the determination of deltamethrin and fenvalerate in human urine is described. Both deltamethrin and fenvalerate have been analysed by gas chromatography with a electron capture detector. The least detectable concentration of both pyrethroids in urine is 0.2 g per litre. The precision and accuracy of the method were within acceptable limits. The method has been used to determine the pyrethroids in urine samples obtained from packers, spraymen and acute pyrethroids poisoning patients展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘A HPLC Assay was developed to identify and measure the metabolite of acrylamide, mercapturic acid, N-Acetyl-s-(propionamide)-cysteine (APC) in urine. O-phthalaldehyde (OPA) was utilized as a precolumn derivatizing agent in the assay. This derivative was isolated with a good selectivity by high performance liquid chromatography (HPLC) employing reversed phase ODS columns. The quantitation of the mercapturic acid derivative was reproducible and with a detection limit of 1 pmol. The average coefficient of variation for the runs carried out on the same day was approximately 4.6% at the range of 80-160 ianol-L'1 of APC, and the mean analytical recovery from urine samples was 94%.The metabolite of urine of workers exposed to acrylamide was identified as N-acetyl-s-(propionamide)- cysteine by HPLC chromatography and fluorescence scan and HPLC-Mass spectra. All results were identical with the authentic synthesized compound.
文摘A gas chromatographic method for the determination of deltamethrin and fenvalerate in human urine is described. Both deltamethrin and fenvalerate have been analysed by gas chromatography with a electron capture detector. The least detectable concentration of both pyrethroids in urine is 0.2 g per litre. The precision and accuracy of the method were within acceptable limits. The method has been used to determine the pyrethroids in urine samples obtained from packers, spraymen and acute pyrethroids poisoning patients
