Isoetes yunguiensis is an endangered and endem-ic fern in China.Field survey indicated that only one popula-tion and no more than 50 individuals occur in the wild.The genetic variation of 46 individuals from the popul...Isoetes yunguiensis is an endangered and endem-ic fern in China.Field survey indicated that only one popula-tion and no more than 50 individuals occur in the wild.The genetic variation of 46 individuals from the population remaining at Pingba(Guizhou Province,China)was assessed by Random Amplified Polymorphic DNA(RAPD)fingerprinting.Twelve primers were screened from sixty ten-bp arbitrary primers,and a total of 95 DNA fragments were scored.Of these,62.1%were polymorphic loci,which indi-cated that high level genetic variation existed in the natural population.The accumulation of genetic variation in the history of the taxon and the apparent minimal reduction effect on genetic diversity following destruction of habitat might be responsible for the high level genetic diversity presently remaining in the I.yunguiensis population.However,with the continuing decrease of population size,the genetic diversity will gradually be lost.We suggest that the materials from the extant population should be used for re-establishment of the populations.展开更多
基金This study was supported by the State Key Basic Research and Development Plan(No.G2000046805)the National Natural Science Foundation of China(Grant No.30370098).
文摘Isoetes yunguiensis is an endangered and endem-ic fern in China.Field survey indicated that only one popula-tion and no more than 50 individuals occur in the wild.The genetic variation of 46 individuals from the population remaining at Pingba(Guizhou Province,China)was assessed by Random Amplified Polymorphic DNA(RAPD)fingerprinting.Twelve primers were screened from sixty ten-bp arbitrary primers,and a total of 95 DNA fragments were scored.Of these,62.1%were polymorphic loci,which indi-cated that high level genetic variation existed in the natural population.The accumulation of genetic variation in the history of the taxon and the apparent minimal reduction effect on genetic diversity following destruction of habitat might be responsible for the high level genetic diversity presently remaining in the I.yunguiensis population.However,with the continuing decrease of population size,the genetic diversity will gradually be lost.We suggest that the materials from the extant population should be used for re-establishment of the populations.
