Association of Fas/Apo1 gene promoter (-670 A/G) polymorphism in Tunisian patients with IBD

AIM： To detect a possible association between the polymorphism of the （-670 A/G） Fas/Apol gene promoter and susceptibility to Crohn＇s disease （CD） and ulcerative colitis （UC） in the Tunisian population. METHODS： The （-670 A/G） Fas polymorphism was analyzed in 105 patients with CD, 59 patients with UC, and 100 controls using the polymerase chain reaction restriction fragment length polymorphism method. RESULTS： Significantly lower frequencies of the Fas -670 A allele and A/A homozygous individuals were observed in CD and UC patients when compared with controls. Analysis of （-670 A/G） Fas polymorphism with respect to sex in CD and UC showed a significant difference in A/A genotypes between female patients and controls （P corrected = 0.004 ＂in CD patients＂ and P corrected = 0.02 ＂in UC patients＂, respectively）. Analysis also showed a statistically significant association between genotype AA of the （-670 A/G） polymorphism and the ileum localization of the lesions （P corrected = 0.048） and between genotype GG and the colon localization （P corrected = 0.009）. The analysis of IBD patients according to clinical behavior revealed no difference. CONCLUSION： Fas-670 polymorphism was associated with the development of CD and UC in the Tunisian population.

Lymphoid tyrosine phosphatase R620W variant and inflammatory bowel disease in Tunisia

AIM:To assess the possible association between PTPN22(R620W) gene polymorphism and inflammatory bowel disease(IBD).METHODS:One hundred and sixty-four patients with IBD 105 Crohn's disease(CD) and 59 ulcerative colitis(UC) and 100 healthy controls were recruited.Genotyping of the PTPN22 gene 1858C→T polymorphism was performed by restriction fragment length polymorphism-polymerase chain reaction with Rsa Ⅰ digestion.RESULTS:The genotypic and allelic frequencies of(R620W) PTPN22 gene polymorphism reveal a significant association of the PTPN22 620-W allele with IBD,compared to the healthy control group(OR:17.81,95% CI:4.18-21.86,P = 0.00001).Nevertheless,nodifference in this polymorphism was found between CD and UC patients.No significant association was found between the frequencies of genotypes of the PTPN22 gene with either the clinical features such as sex,age,age at disease onset,and extent of colitis,or the production of serological markers(anti-Saccharomyces cerevisiae antibody in CD and perinuclear anti-neutrophil cytoplasmic antibody in UC).CONCLUSION:These observations confirm the association of IBD susceptibility with the PTPN22 1858T(620-W) allele in Tunisian patients.