Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber developme...Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.展开更多
BACKGROUND Acute myeloid leukemia(AML)is a disease in which immature hematopoietic cells accumulate in the bone marrow and continuously expand,inhibiting hematopoiesis.The treatment and prognosis of this disease have ...BACKGROUND Acute myeloid leukemia(AML)is a disease in which immature hematopoietic cells accumulate in the bone marrow and continuously expand,inhibiting hematopoiesis.The treatment and prognosis of this disease have always been unsatisfactory.AIM To investigate the correlation between vascular endothelial growth factor(VEGF)and transforming growth factor-β1(TGFβ1)expression and prognosis in older adults with AML.METHODS This study enrolled 80 patients with AML(AML group),including 36 with complete response(AML-CR),23 with partial response(AML-PR),and 21 with no response(AML-NR).The expression levels of VEGF and TGFβ1 were detected by reverse transcription polymerase chain reaction in bone marrow mononuclear cells isolated from 56 healthy controls.Kaplan-Meier analysis was performed to assess overall survival(OS)and progression-or disease-free survival(DFS).Prognostic risk factors were analyzed using a Cox proportional hazards model.RESULTS The AML group showed a VEGF level of 2.68±0.16.VEGF expression was lower in patients with AML-CR than those with AML-PR or AML-NR(P<0.05).TGFβ1 expression in the AML group was 0.33±0.05.Patients with AML-CR showed a higher TGFβ1 expression than those with AML-PR or AML-NR(P<0.05).VEGF and TGFβ1 expression in patients with AML was significantly correlated with the counts of leukocytes,platelets,hemoglobin,and peripheral blood immature cells(P<0.05);Kaplan-Meier survival analysis revealed that patients with high TGFβ1 expression had better OS and DFS than those with low TGFβ1 expression(P<0.05),whereas patients with low VEGF levels showed better OS and DFS than those with high VEGF levels(P<0.05).VEGF,TGFβ1,and platelet count were identified by the Cox proportional hazards model as independent risk factors for OS(P<0.05),while VEGF,TGFβ1,and white blood cell count were independent risk factors for DFS(P<0.05).CONCLUSION Decreased VEGF expression and increased TGFβ1 expression in patients with AML provide valuable references for determining and individualizing clinical treatment strategies.展开更多
Poliumoside is the main active constituent of Callicarpa kwangtungensis Chun (CK), a traditional Chinese medicine for management of hemostasis. In this study, a rapid and selective ultra-performance liquid chromatogra...Poliumoside is the main active constituent of Callicarpa kwangtungensis Chun (CK), a traditional Chinese medicine for management of hemostasis. In this study, a rapid and selective ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/ Q-TOF-MS) method was developed and validated to quantify poliumoside in rat plasma. The targeted analytes in rat plasma were prepared through protein-precipitation method using 10% trichloroacetic acid (TCA). The chromatographic separation was performed on a Waters BEH C<sub>18</sub> column (2.1 × 100 mm, 1.7 μm) by acetonitrile-water containing 0.1% formic acid. The calibration curve was linear over the range of 50 - 10,000 ng/mL (r<sup>2</sup> > 0.99). The intra-day or inter-day precision was less than 7.97% and accuracy was within ?7.00% - 3.36%. The developed method was successfully applied to pharmacokinetic study of poliumoside in rat plasma. Although being rapidly absorbed (T<sub>max</sub> ≤ 30 min), poliumoside was poorly bioavailable after oral administration (the absolute bioavailability was only 0.69%).展开更多
A series of element concentrations sampled from four drill cores with a length about 1000 m into different skarn-type deposits were selected from the Shizishan orefield, central Tongling, southeastern part of Anhui Pr...A series of element concentrations sampled from four drill cores with a length about 1000 m into different skarn-type deposits were selected from the Shizishan orefield, central Tongling, southeastern part of Anhui Province. Using the multifractal method, the distribution and migration characteristics of the major and trace elements are analyzed. The multifractal spectrum of the major elements is left-skewed, whereas the spectrum of the trace elements is right-skewed, which shows that in the process of skarn formation, the trace elements were enriched only locally, and major elements transported within a much larger range. The correlation coefficients of the multifractal parameters Aa （width of the multifractal spectrum） of the four drill cores are relatively low, but the correlation coefficients of the multifractal parameters R （spectrum symmetry parameter） and Af are relatively higher, indicating that although the non-homogeneous intensity of the distribution of elements is inconsistent, their spatial accumulation patterns are almost the same during the ore-forming process. The statistics of the mnltifractal parameters of various elements in the different locations show that the ore-forming processes and element migration pattern in the Shizishan orefield are consistent, and that the migrations of trace elements and major elements exhibit some differences.展开更多
The research studied the combined effects of ultrasonic vibration （USV） and manganese on the Fe-containing inter-metallic compounds and mechanical properties of AI-17Si-3Fe-2Cu-1Ni （wt.%） alloys. The results showe...The research studied the combined effects of ultrasonic vibration （USV） and manganese on the Fe-containing inter-metallic compounds and mechanical properties of AI-17Si-3Fe-2Cu-1Ni （wt.%） alloys. The results showed that, without USV, the alloys with 0.4wt.% Mn or 0.8wt.% Mn both contain a large amount of coarse plate-like δ-AI4（Fe,Mn）Si2 phase and long needle-like β-A15（Fe,Mn）Si phase. When the Mn content changes from 0.4wt.% to 0.8wt.% in the alloys, the amount and the length of needle-like β-AI5（Fe,Mn）Si phase decrease and the plate-like δ-A14（Fe,Mn）Si2 phase becomes much coarser. After USV treatment, the Fe- containing compounds in the alloys are refined and exist mainly as δ-AI4（Fe,Mn）Si2 particles with an average grain size of about 20μm, and only a small amount of β-AI5（Fe,Mn）Si phase remains. With USV treatment, the ultimate tensile strengths （UTS） of the alloys containing 0.4wt.%Mn and 0.8wt.%Mn at room temperature are 253 MPa and 262 MPa, respectively, and the ultimate tensile strengths at 350 ℃ are 129 MPa and 135 MPa, respectively. It is considered that the modified morphology and uniform distribution of the Fe-containing inter-metallic compounds, which are caused by the USV process, are the main reasons for the increase in the tensile strength of these two alloys.展开更多
Heterogeneity of permeability in fractured media is a hot research topic in hydrogeology. Numerous approaches have been proposed to characterize heterogeneity in the last several decades. However, little attention has...Heterogeneity of permeability in fractured media is a hot research topic in hydrogeology. Numerous approaches have been proposed to characterize heterogeneity in the last several decades. However, little attention has been paid to correlate permeability heterogeneity with geological information. In the present study, several causes of permeability heterogeneity, that is, lithology, tectonism, and depth, are identified. The unit absorption values （denoted as ω）, which are results obtained from the packer test, are employed to represent permeability. The variability of permeability in sandstone-mudstone is so significant that the value of unit absorptions span 3-4 orders of magnitude at any depth with several test sections. By declustering, it has been found that under a similar tectonic history, the means of permeability differ greatly at different formations as a result of different mudrock contents. It has also been found that in the same formation, permeability can be significantly increased as a result of faulting. The well-known phenomenon, the decrease in permeability with depth, is found to be caused by the fractures in the rock mass, and the relationship between permeability and depth can be established in the form of logoω-logd. After subtracting the trend of ω with absolute depth, the mean of the residual value at each relative depth can be well correlated with the distribution of mudstone. The methods proposed in this paper can be utilized to research in similar study areas.展开更多
Heilongjiang Province is the main soybean-producing area in china. In this study, we analyzed the canopy structure, dynamic characteristics of light radiation and yield of Hefeng50 (the main variety of soybean in Heil...Heilongjiang Province is the main soybean-producing area in china. In this study, we analyzed the canopy structure, dynamic characteristics of light radiation and yield of Hefeng50 (the main variety of soybean in Heilongjiang Province) under six different cultivation patterns (ORP, TPCR, ORCP, BRHD, SRHD and FPHD). The results showed that SRHD and BRHD at different growth period (blossom period R1, podding R3 and grain filing period R5) produced an even distribution of the population leaf area, suitable mean foliage inclination angle (MFIA), low transparency coefficients for defuse penetration (TCDP) and transparency coefficients for radiation penetration (TCRP), high leaf area index (LAI), extinction light coefficient (K value), fraction of radiation intercepted (FRI) and light energy utilization rate. Grain number, dry matter weight per plant, and yield of SRHD and BRHD were significantly higher than those of other cultivation patterns. The yield of SRHD, BRHD, ORCP, FPHD and TPCR was increased by 136%, 112%, 79%, 50.1% and 14.7%, respectively, compared to that of ORP. These results suggest that SRHD and BRHD are the optimal cultivation pattern for the improvement of soybean yield in phaeozem region of northeastern China.展开更多
Glioblastoma(GBM) is the most common,malignant,and lethal primary brain tumor in adults.Up to now,there is no effective drug for GBM.Withaferin A(WFA) is mainly derived from Indian Winter cherry.It has been traditiona...Glioblastoma(GBM) is the most common,malignant,and lethal primary brain tumor in adults.Up to now,there is no effective drug for GBM.Withaferin A(WFA) is mainly derived from Indian Winter cherry.It has been traditionally used in ayurvedic medicine.WFA has wide range of pharmaco.logical activities including cardioprotective,anti-inflammatory,immuno-modulatory properties.Recently,WFA was reported to inhibit the growth of many cancer cells;however,the precise molecular mecha.nisms of its anti-cancer activities in GBM remain unclear.Here,we found that treatment of WFA in U251 and U87-MG glioma cells inhibited the cell proliferation,released the cellular LDH,decreased the DNA synthesis,and inhibited the migration,invasion,and colony formation of cells.WFA also in.creased the apoptotic rate of cells,decreased the mitochondrial membrane potential,arrested cell cy.cle at G_2/M,inhibited the activity of caspase 3/7,and increased the protein expression of cleaved-cas.pase 3,cleaved PARP in U251 and U87-MG cells.In addition,cell apoptosis induced by WFA was as.sociated with increasing level of Bim,Bad,P21,P53 and decreasing the level of p-CDK1,cyclin A and B.It was also shown that cell apoptosis induced by WFA was associated with P38 signal pathway.These results demonstrated that WFA induced mitochondrial dependent apoptosis in glioblastoma cells which was associated with arresting the cell cycle at G_2/M phase by P38 pathway.Taken together,our findings suggest that WFA might be a promising chemotherapy drug in the treatment of GBM.展开更多
基金supported by the National Natural Science Foundation of China(31801409)the Safe Preservation and Accurate Identification of Flax Germplasm Resources in South,China(23ZH174)+2 种基金the Construction of Modern Agricultural Industrial Technology System,China(CARS-16-E01)the Protection and Utilization of Crop Germplasm Resources,China(2016NWB044)the National Science and Technology Resource Sharing Service Platform Project,China(NCGRC-2020-15)。
文摘Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.
基金the Ethic Committee of Suzhou Hospital of Anhui Medical University(Approval No.C2024003).
文摘BACKGROUND Acute myeloid leukemia(AML)is a disease in which immature hematopoietic cells accumulate in the bone marrow and continuously expand,inhibiting hematopoiesis.The treatment and prognosis of this disease have always been unsatisfactory.AIM To investigate the correlation between vascular endothelial growth factor(VEGF)and transforming growth factor-β1(TGFβ1)expression and prognosis in older adults with AML.METHODS This study enrolled 80 patients with AML(AML group),including 36 with complete response(AML-CR),23 with partial response(AML-PR),and 21 with no response(AML-NR).The expression levels of VEGF and TGFβ1 were detected by reverse transcription polymerase chain reaction in bone marrow mononuclear cells isolated from 56 healthy controls.Kaplan-Meier analysis was performed to assess overall survival(OS)and progression-or disease-free survival(DFS).Prognostic risk factors were analyzed using a Cox proportional hazards model.RESULTS The AML group showed a VEGF level of 2.68±0.16.VEGF expression was lower in patients with AML-CR than those with AML-PR or AML-NR(P<0.05).TGFβ1 expression in the AML group was 0.33±0.05.Patients with AML-CR showed a higher TGFβ1 expression than those with AML-PR or AML-NR(P<0.05).VEGF and TGFβ1 expression in patients with AML was significantly correlated with the counts of leukocytes,platelets,hemoglobin,and peripheral blood immature cells(P<0.05);Kaplan-Meier survival analysis revealed that patients with high TGFβ1 expression had better OS and DFS than those with low TGFβ1 expression(P<0.05),whereas patients with low VEGF levels showed better OS and DFS than those with high VEGF levels(P<0.05).VEGF,TGFβ1,and platelet count were identified by the Cox proportional hazards model as independent risk factors for OS(P<0.05),while VEGF,TGFβ1,and white blood cell count were independent risk factors for DFS(P<0.05).CONCLUSION Decreased VEGF expression and increased TGFβ1 expression in patients with AML provide valuable references for determining and individualizing clinical treatment strategies.
文摘Poliumoside is the main active constituent of Callicarpa kwangtungensis Chun (CK), a traditional Chinese medicine for management of hemostasis. In this study, a rapid and selective ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/ Q-TOF-MS) method was developed and validated to quantify poliumoside in rat plasma. The targeted analytes in rat plasma were prepared through protein-precipitation method using 10% trichloroacetic acid (TCA). The chromatographic separation was performed on a Waters BEH C<sub>18</sub> column (2.1 × 100 mm, 1.7 μm) by acetonitrile-water containing 0.1% formic acid. The calibration curve was linear over the range of 50 - 10,000 ng/mL (r<sup>2</sup> > 0.99). The intra-day or inter-day precision was less than 7.97% and accuracy was within ?7.00% - 3.36%. The developed method was successfully applied to pharmacokinetic study of poliumoside in rat plasma. Although being rapidly absorbed (T<sub>max</sub> ≤ 30 min), poliumoside was poorly bioavailable after oral administration (the absolute bioavailability was only 0.69%).
文摘A series of element concentrations sampled from four drill cores with a length about 1000 m into different skarn-type deposits were selected from the Shizishan orefield, central Tongling, southeastern part of Anhui Province. Using the multifractal method, the distribution and migration characteristics of the major and trace elements are analyzed. The multifractal spectrum of the major elements is left-skewed, whereas the spectrum of the trace elements is right-skewed, which shows that in the process of skarn formation, the trace elements were enriched only locally, and major elements transported within a much larger range. The correlation coefficients of the multifractal parameters Aa （width of the multifractal spectrum） of the four drill cores are relatively low, but the correlation coefficients of the multifractal parameters R （spectrum symmetry parameter） and Af are relatively higher, indicating that although the non-homogeneous intensity of the distribution of elements is inconsistent, their spatial accumulation patterns are almost the same during the ore-forming process. The statistics of the mnltifractal parameters of various elements in the different locations show that the ore-forming processes and element migration pattern in the Shizishan orefield are consistent, and that the migrations of trace elements and major elements exhibit some differences.
基金funded by Project 51275183 supported by the National Natural Science Foundation of Chinaby the National Basic Research Program of China(973Program)(No.2012CB619600)
文摘The research studied the combined effects of ultrasonic vibration （USV） and manganese on the Fe-containing inter-metallic compounds and mechanical properties of AI-17Si-3Fe-2Cu-1Ni （wt.%） alloys. The results showed that, without USV, the alloys with 0.4wt.% Mn or 0.8wt.% Mn both contain a large amount of coarse plate-like δ-AI4（Fe,Mn）Si2 phase and long needle-like β-A15（Fe,Mn）Si phase. When the Mn content changes from 0.4wt.% to 0.8wt.% in the alloys, the amount and the length of needle-like β-AI5（Fe,Mn）Si phase decrease and the plate-like δ-A14（Fe,Mn）Si2 phase becomes much coarser. After USV treatment, the Fe- containing compounds in the alloys are refined and exist mainly as δ-AI4（Fe,Mn）Si2 particles with an average grain size of about 20μm, and only a small amount of β-AI5（Fe,Mn）Si phase remains. With USV treatment, the ultimate tensile strengths （UTS） of the alloys containing 0.4wt.%Mn and 0.8wt.%Mn at room temperature are 253 MPa and 262 MPa, respectively, and the ultimate tensile strengths at 350 ℃ are 129 MPa and 135 MPa, respectively. It is considered that the modified morphology and uniform distribution of the Fe-containing inter-metallic compounds, which are caused by the USV process, are the main reasons for the increase in the tensile strength of these two alloys.
基金support by the National Natural Science Foundation of China(No.40528003 and 50639090)
文摘Heterogeneity of permeability in fractured media is a hot research topic in hydrogeology. Numerous approaches have been proposed to characterize heterogeneity in the last several decades. However, little attention has been paid to correlate permeability heterogeneity with geological information. In the present study, several causes of permeability heterogeneity, that is, lithology, tectonism, and depth, are identified. The unit absorption values （denoted as ω）, which are results obtained from the packer test, are employed to represent permeability. The variability of permeability in sandstone-mudstone is so significant that the value of unit absorptions span 3-4 orders of magnitude at any depth with several test sections. By declustering, it has been found that under a similar tectonic history, the means of permeability differ greatly at different formations as a result of different mudrock contents. It has also been found that in the same formation, permeability can be significantly increased as a result of faulting. The well-known phenomenon, the decrease in permeability with depth, is found to be caused by the fractures in the rock mass, and the relationship between permeability and depth can be established in the form of logoω-logd. After subtracting the trend of ω with absolute depth, the mean of the residual value at each relative depth can be well correlated with the distribution of mudstone. The methods proposed in this paper can be utilized to research in similar study areas.
文摘Heilongjiang Province is the main soybean-producing area in china. In this study, we analyzed the canopy structure, dynamic characteristics of light radiation and yield of Hefeng50 (the main variety of soybean in Heilongjiang Province) under six different cultivation patterns (ORP, TPCR, ORCP, BRHD, SRHD and FPHD). The results showed that SRHD and BRHD at different growth period (blossom period R1, podding R3 and grain filing period R5) produced an even distribution of the population leaf area, suitable mean foliage inclination angle (MFIA), low transparency coefficients for defuse penetration (TCDP) and transparency coefficients for radiation penetration (TCRP), high leaf area index (LAI), extinction light coefficient (K value), fraction of radiation intercepted (FRI) and light energy utilization rate. Grain number, dry matter weight per plant, and yield of SRHD and BRHD were significantly higher than those of other cultivation patterns. The yield of SRHD, BRHD, ORCP, FPHD and TPCR was increased by 136%, 112%, 79%, 50.1% and 14.7%, respectively, compared to that of ORP. These results suggest that SRHD and BRHD are the optimal cultivation pattern for the improvement of soybean yield in phaeozem region of northeastern China.
基金supported by National Natural Science Foundation of China(8157345481703536+2 种基金81703565) CAMS Innovation Fund for Medical Sciences(2016-I2M-3-007) Natural Science Foundation of Beijing(7172142)
文摘Glioblastoma(GBM) is the most common,malignant,and lethal primary brain tumor in adults.Up to now,there is no effective drug for GBM.Withaferin A(WFA) is mainly derived from Indian Winter cherry.It has been traditionally used in ayurvedic medicine.WFA has wide range of pharmaco.logical activities including cardioprotective,anti-inflammatory,immuno-modulatory properties.Recently,WFA was reported to inhibit the growth of many cancer cells;however,the precise molecular mecha.nisms of its anti-cancer activities in GBM remain unclear.Here,we found that treatment of WFA in U251 and U87-MG glioma cells inhibited the cell proliferation,released the cellular LDH,decreased the DNA synthesis,and inhibited the migration,invasion,and colony formation of cells.WFA also in.creased the apoptotic rate of cells,decreased the mitochondrial membrane potential,arrested cell cy.cle at G_2/M,inhibited the activity of caspase 3/7,and increased the protein expression of cleaved-cas.pase 3,cleaved PARP in U251 and U87-MG cells.In addition,cell apoptosis induced by WFA was as.sociated with increasing level of Bim,Bad,P21,P53 and decreasing the level of p-CDK1,cyclin A and B.It was also shown that cell apoptosis induced by WFA was associated with P38 signal pathway.These results demonstrated that WFA induced mitochondrial dependent apoptosis in glioblastoma cells which was associated with arresting the cell cycle at G_2/M phase by P38 pathway.Taken together,our findings suggest that WFA might be a promising chemotherapy drug in the treatment of GBM.
