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流行性乙型脑炎病毒SA14-14-2株囊膜蛋白的原核表达及其兔抗血清的制备 被引量:1
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作者 徐飞 冯继伟 +4 位作者 李鹏 孟玉娇 陈玉明 王龙康 王寅彪 《新乡医学院学报》 CAS 2022年第2期101-105,共5页
目的表达流行性乙型脑炎病毒(EEBV)的囊膜蛋白(E蛋白),制备该蛋白特异性兔抗血清,为建立诊断EEBV感染的检测方法奠定基础。方法将pET32a-E质粒转化大肠杆菌BL21(DE3)细胞,以终浓度为0.5 mmol·L^(-1)的异丙基-β-D-硫代半乳糖苷于3... 目的表达流行性乙型脑炎病毒(EEBV)的囊膜蛋白(E蛋白),制备该蛋白特异性兔抗血清,为建立诊断EEBV感染的检测方法奠定基础。方法将pET32a-E质粒转化大肠杆菌BL21(DE3)细胞,以终浓度为0.5 mmol·L^(-1)的异丙基-β-D-硫代半乳糖苷于37℃下诱导蛋白表达8 h,在8 mol·L^(-1)尿素变性条件下,采用镍离子金属螯合亲和层析法对重组E蛋白进行纯化。透析复性后,以E蛋白为免疫原免疫新西兰兔制备兔抗血清,采用Western blot、酶联免疫吸附试验和免疫荧光分析法检测免疫血清中EEBV特异性抗体。结果成功表达了重组E蛋白,经纯化、复性后可从1 L培养物中获得23.8 mg具有生物活性的E蛋白,该蛋白能与抗EEBV单抗发生特异反应。免疫制备的兔抗血清能与E蛋白发生特异反应,抗体效价高达1×51 200;兔抗血清能与EEBV感染的细胞发生特异反应。结论成功表达了EEBV E蛋白并制备了特异性强、亲和力高的兔抗血清,为建立诊断EEBV感染的免疫学检测方法、有效防控流行性乙型脑炎奠定了基础。 展开更多
关键词 流行性乙型脑炎病毒 囊膜蛋白 原核表达 兔抗血清
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Development of a spontaneous combustion TARPs system based on BP neural network 被引量:7
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作者 wang longkang Ren Tingxiang +4 位作者 Nie Baisheng Chen Yang Lv Changqing Tang Haoyang Zhang Jufeng 《International Journal of Mining Science and Technology》 SCIE EI CSCD 2015年第5期803-810,共8页
Spontaneous combustion of coal is a major cause of coal mine fires.It not only poses a severe hazard to the safe extraction of coal resources,but also jeopardizes the safety of mine workers.The development of a scient... Spontaneous combustion of coal is a major cause of coal mine fires.It not only poses a severe hazard to the safe extraction of coal resources,but also jeopardizes the safety of mine workers.The development of a scientific management system of coal spontaneous combustion is of vital importance to the safe production of coal mine.This paper provides a comparative analysis of a range of worldwide prediction techniques and methods for coal spontaneous combustion,and systematically introduces the trigger action response plans(TARPs)system used in Australian coal mines for managing the spontaneous heating of coal.An artificial neural network model has been established on the basis of real coal mine operational conditions.Through studying and training the neural network model,prediction errors can be controlled within the allowable range.The trained model is then applied to the conditions of Nos.1 and 3 coal seams located in Weijiadi Coal Mine to demonstrate its feasibility for spontaneous combustion assessment.Based upon the TARPs system which is commonly used in Australian longwall mines,a TARPs system has been developed for Weijiadi Coal Mine to assist the management of spontaneous combustion hazard and ensure the safe operation of its mining activities. 展开更多
关键词 管理系统 BP神经网络 人工神经网络模型 煤炭自燃 煤矿自燃 燃油 澳大利亚 危险性管理
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Simulation of the interaction of methane,carbon dioxide and coal 被引量:1
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作者 Nie Baisheng wang longkang +2 位作者 Li Xiangchun wang Chao Li Li 《International Journal of Mining Science and Technology》 SCIE EI 2013年第6期919-923,共5页
Gas adsorption has an important influence on gas flow in a coal body.Research on the characteristics of coal and gas adsorption is the theoretical basis for studying gas flow in coal.In this paper,the interaction betw... Gas adsorption has an important influence on gas flow in a coal body.Research on the characteristics of coal and gas adsorption is the theoretical basis for studying gas flow in coal.In this paper,the interaction between methane,carbon dioxide and surface molecules of anthracite was simulated using the quantum chemistry method.Adsorption energy and adsorption configurations of different quantities of gas molecules absorbed on the coal surface were calculated.The results show that adsorption between coal and the two kinds of gas molecules is a physical adsorption process and there is an optimal configuration.Gas molecules are more easily adsorbed in the hydroxyl-containing side chain,while it is difficult for them to be adsorbed at the position of the benzene ring.Besides,carbon dioxide molecules are more readily adsorbed on the coal surface than methane molecules.The findings have an important significance in revealing the nature of gas adsorption in coal. 展开更多
关键词 METHANE Carbon dioxide Coal surface molecules Interaction energy Quantum chemistry
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