To improve the hydrogen evolution rate in continuous hydrogen production of a novel fermentative hydrogen producing bacteria strain B49 (AF481148 in EMBL), 4 % immobilized cells by polyvinyl alcohol boric acid method,...To improve the hydrogen evolution rate in continuous hydrogen production of a novel fermentative hydrogen producing bacteria strain B49 (AF481148 in EMBL), 4 % immobilized cells by polyvinyl alcohol boric acid method, with the addition of a small amount of calcium alginate in a column reactor obtain hydrogen yield of 2.31 mol H2/mol glucose and hydrogen evolution rate of 1435.4 ml/L culture·h respectively at medium retention time of 2.0 h with a medium containing 10g glucose/L. Moreover, as the cell density in gel beads is increased to 8%, hydrogen yield and hydrogen evolution rate for 10g glucose/L are 2.34 mol H2/mol glucose and 2912.4 ml/L culture·h respectively at medium retention time of 1.0 h, and for molasses wastewater COD of 7505.9 mg/L hydrogen production potential of 205.6 ml/g COD and hydrogen evolution rate of 2057.7 ml/L culture·h at hydraulic retention time of 0.75 h are observed. In the continuous culture pH value keeps around 3.9 by self regulating.展开更多
In order to screen a high-yield spergualin-production strain and an optimal fermentation medium, large numbers of isolates were selected after ultraviolet （UV） mutation and self-tolerant mutation of Bacillus lateros...In order to screen a high-yield spergualin-production strain and an optimal fermentation medium, large numbers of isolates were selected after ultraviolet （UV） mutation and self-tolerant mutation of Bacillus laterosporus A7. A high-yield strain A-94-7 was obtained and the spergualin productivity was 4. 1-fold of the parent strain. The genetic stability of the high-yield strain Bacillus laterosporus A-94-7 was very good. Aider fermentation medium optimization, Bacillus laterosporus A-94-7 was able to produce 380 mg· L^-1 The spergualin productivity in Bacillus laterosporus A7 was increased 4.75-fold by mutation and medium optimization.展开更多
The aim of this work was to develop a mutation and selection procedure to select the high-yield strain, and this strain could alleviate the inhibition in the biosynthesis of ramoplanin caused by L-leucine. In this exp...The aim of this work was to develop a mutation and selection procedure to select the high-yield strain, and this strain could alleviate the inhibition in the biosynthesis of ramoplanin caused by L-leucine. In this experiment, the strain Actinoplanes sp. C6-78-14 that produced 384.8 mg of total productivity per liter was isolated. Its ramoplanin productivity was 4.13-fold higher than that of the parent strain A ctinoplanes sp. C2-9-45. The subculture experiments showed that the hereditary character of C6-78-14 was stable. Subsequent media studies with strain Actinoplanes sp. C6-78-14 showed that adding 0.001% FeSO4· 7H2O, 0.001% CoCl2· 6H2O and 0.001% CuSO4· 5H2O to fermentation medium respectively could enhance the ramoplanin production, and the production of each group was increased by 10%, 15% and 20% compared with the control group.展开更多
文摘To improve the hydrogen evolution rate in continuous hydrogen production of a novel fermentative hydrogen producing bacteria strain B49 (AF481148 in EMBL), 4 % immobilized cells by polyvinyl alcohol boric acid method, with the addition of a small amount of calcium alginate in a column reactor obtain hydrogen yield of 2.31 mol H2/mol glucose and hydrogen evolution rate of 1435.4 ml/L culture·h respectively at medium retention time of 2.0 h with a medium containing 10g glucose/L. Moreover, as the cell density in gel beads is increased to 8%, hydrogen yield and hydrogen evolution rate for 10g glucose/L are 2.34 mol H2/mol glucose and 2912.4 ml/L culture·h respectively at medium retention time of 1.0 h, and for molasses wastewater COD of 7505.9 mg/L hydrogen production potential of 205.6 ml/g COD and hydrogen evolution rate of 2057.7 ml/L culture·h at hydraulic retention time of 0.75 h are observed. In the continuous culture pH value keeps around 3.9 by self regulating.
基金Supported by Program for Innovative Research Team of Northeast Agricultural University
文摘In order to screen a high-yield spergualin-production strain and an optimal fermentation medium, large numbers of isolates were selected after ultraviolet （UV） mutation and self-tolerant mutation of Bacillus laterosporus A7. A high-yield strain A-94-7 was obtained and the spergualin productivity was 4. 1-fold of the parent strain. The genetic stability of the high-yield strain Bacillus laterosporus A-94-7 was very good. Aider fermentation medium optimization, Bacillus laterosporus A-94-7 was able to produce 380 mg· L^-1 The spergualin productivity in Bacillus laterosporus A7 was increased 4.75-fold by mutation and medium optimization.
基金Program for Innovative Research Team of Northeast Agricultural University
文摘The aim of this work was to develop a mutation and selection procedure to select the high-yield strain, and this strain could alleviate the inhibition in the biosynthesis of ramoplanin caused by L-leucine. In this experiment, the strain Actinoplanes sp. C6-78-14 that produced 384.8 mg of total productivity per liter was isolated. Its ramoplanin productivity was 4.13-fold higher than that of the parent strain A ctinoplanes sp. C2-9-45. The subculture experiments showed that the hereditary character of C6-78-14 was stable. Subsequent media studies with strain Actinoplanes sp. C6-78-14 showed that adding 0.001% FeSO4· 7H2O, 0.001% CoCl2· 6H2O and 0.001% CuSO4· 5H2O to fermentation medium respectively could enhance the ramoplanin production, and the production of each group was increased by 10%, 15% and 20% compared with the control group.
