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Sequencing of p35 Gene from Heliothis armigera Polyhedrosis Virus and Its Homologous Comparison
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作者 wang ye-fu Qi Yi-peng +2 位作者 Li Zhi-da Zhu Ying Su fei 《Wuhan University Journal of Natural Sciences》 CAS 1999年第2期123-129,共7页
Using p35 gene primers of AcNPV, about 1 kb fragment was obtained by PCR from HaNPV DNA and was sequenced thereafter. It has a full reading frame encode 299 amino acids. Sharing identity of 94% in nucleotides and 84%... Using p35 gene primers of AcNPV, about 1 kb fragment was obtained by PCR from HaNPV DNA and was sequenced thereafter. It has a full reading frame encode 299 amino acids. Sharing identity of 94% in nucleotides and 84% in predict amino acids with AcNPV, a apoptosis inhibiting gene was found. According to comparison of p35 genes in five kinds of baculovirus, it is found that AcNPV, TnNPV and BmNPV share the most intimate blood relation. HaNPV is near the above three species. LsNPV is little remote from them. This result reconfirmed those we have done with gp37 genes and vp39 genes. It is more accurate to use conserved gene for species division than that of the serological identification. 展开更多
关键词 molecular evolution homologous comparison PCR amplification
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