In recent years, the occurrence of rice false smut has become increasingly serious and the area of damage has expanded year by year, so the false smut has become one of the diseases which have seriously affected rice ...In recent years, the occurrence of rice false smut has become increasingly serious and the area of damage has expanded year by year, so the false smut has become one of the diseases which have seriously affected rice yield and quality in Heilongjiang Province. However, the genetic diversity research on false smut in Heilongjiang Province was relatively weak. In the current investigation, seven polymorphic SSR markers were taken to analyze the genetic diversity among 89 strains of the pathogens of rice false smut collected from five main rice growing areas in Heilongjiang Province, China. The results showed that the amplified bands of each pair of primers were between 2 and 7. A total of 43 bands were obtained, and each primer was expanded for 4.8 bands. The genetic similarity coefficient(GSC) between strains by using SSR molecular marker analysis showed that the GSC of the strain of Ustilaginoidea virens was 0.613-0.955, with an average of 0.741. According to the results cluster analysis, when the genetic distance was 0.73, 89 strains were divided into three groups, and five strains of rice smut strains were distributed in three populations, and strains in Harbin City were mainly distributed in group Ⅲ. In Mudanjiang City, the strains were mainly distributed in groups Ⅰ and Ⅲ, and the strains in Suihua City were mainly distributed in groups Ⅰ and Ⅱ, and the strains in Jiamusi City were evenly distributed in groups Ⅰ, Ⅱ and Ⅲ, and the strains in Qiqihar City were mainly distributed in groups Ⅱ and Ⅲ. The results showed that the genetic background of Ustilaginoidea virens in Heilongjiang Province was relatively low in complexity, and the overall inheritance of the strain was relatively stable.展开更多
Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to es...Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.展开更多
背景:骨髓间充质干细胞是存在于骨髓中的一种具有多向分化潜能的干细胞,当受到含药血清作用时,其所涉及的多条信号通路上的基因表达会发生改变。目的:考察复方接骨片和壮骨片含药血清在骨髓间充质干细胞体外分化过程中的作用。方法:取60...背景:骨髓间充质干细胞是存在于骨髓中的一种具有多向分化潜能的干细胞,当受到含药血清作用时,其所涉及的多条信号通路上的基因表达会发生改变。目的:考察复方接骨片和壮骨片含药血清在骨髓间充质干细胞体外分化过程中的作用。方法:取60只SD大鼠,随机分为复方接骨片组、壮骨片组、两药联用组和空白对照组,制备含药血清。分离培养SD大鼠骨髓间充质干细胞,于培养瓶中加入不同的含药血清,培养24 h后,RT-PCR法检测BMP-2、Runx2、VEGF及Akt m RNA的表达,Western blot法检测BMP-2、Runx2、VEGF及Akt蛋白的表达。结果与结论:(1)与对照组比较,复方接骨片组及两药联用组Akt和VEGF m RNA及蛋白的表达明显升高(P<0.05或P<0.01);(2)壮骨片组与对照组相比,Akt和VEGF m RNA及蛋白表达差异无显著性意义(P>0.05);(3)与对照组比较,复方接骨片组BMP-2和Runx2 m RNA及蛋白表达升高(P<0.05);(4)与对照组相比,壮骨片组及两药联用组BMP-2及Runx2 m RNA及蛋白表达均显著增加(P<0.01);(5)结果表明,复方接骨片及壮骨片含药血清可在骨髓间充质干细胞体外分化通路中起到协同促进骨髓间充质干细胞向血管内皮细胞及成骨细胞分化的作用。展开更多
基金Project (2020YFF0304103-03) supported by the National Key Researsh and Development Program of ChinaProjects (P2019J023,P2020J025) supported by the Science and Technology Research Program of China State Railway Group Co.,Ltd.+1 种基金Project (202045014) supported by the Initial Funding of Specially-Appointed Professorship of Central South University,ChinaProject (2023ZZTS0424) supported by the Independent Exploration and Innovation Project for Graduate Students of Central South University,China。
基金Supported by the Natural Science Foundation of Heilongjiang Province(C2017032)Applied Technology Research and Development Program of Heilongjiang Province(GA19B104)。
文摘In recent years, the occurrence of rice false smut has become increasingly serious and the area of damage has expanded year by year, so the false smut has become one of the diseases which have seriously affected rice yield and quality in Heilongjiang Province. However, the genetic diversity research on false smut in Heilongjiang Province was relatively weak. In the current investigation, seven polymorphic SSR markers were taken to analyze the genetic diversity among 89 strains of the pathogens of rice false smut collected from five main rice growing areas in Heilongjiang Province, China. The results showed that the amplified bands of each pair of primers were between 2 and 7. A total of 43 bands were obtained, and each primer was expanded for 4.8 bands. The genetic similarity coefficient(GSC) between strains by using SSR molecular marker analysis showed that the GSC of the strain of Ustilaginoidea virens was 0.613-0.955, with an average of 0.741. According to the results cluster analysis, when the genetic distance was 0.73, 89 strains were divided into three groups, and five strains of rice smut strains were distributed in three populations, and strains in Harbin City were mainly distributed in group Ⅲ. In Mudanjiang City, the strains were mainly distributed in groups Ⅰ and Ⅲ, and the strains in Suihua City were mainly distributed in groups Ⅰ and Ⅱ, and the strains in Jiamusi City were evenly distributed in groups Ⅰ, Ⅱ and Ⅲ, and the strains in Qiqihar City were mainly distributed in groups Ⅱ and Ⅲ. The results showed that the genetic background of Ustilaginoidea virens in Heilongjiang Province was relatively low in complexity, and the overall inheritance of the strain was relatively stable.
基金Supported by the Natural Science Foundation of Heilongjiang Province(Topic C2017032)Heilongjiang Province Applied Technology Research and Development Program(Topic GA19B104)the National Key Research and Development Program(Topic 2018YFD0300105)。
文摘Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.
文摘背景:骨髓间充质干细胞是存在于骨髓中的一种具有多向分化潜能的干细胞,当受到含药血清作用时,其所涉及的多条信号通路上的基因表达会发生改变。目的:考察复方接骨片和壮骨片含药血清在骨髓间充质干细胞体外分化过程中的作用。方法:取60只SD大鼠,随机分为复方接骨片组、壮骨片组、两药联用组和空白对照组,制备含药血清。分离培养SD大鼠骨髓间充质干细胞,于培养瓶中加入不同的含药血清,培养24 h后,RT-PCR法检测BMP-2、Runx2、VEGF及Akt m RNA的表达,Western blot法检测BMP-2、Runx2、VEGF及Akt蛋白的表达。结果与结论:(1)与对照组比较,复方接骨片组及两药联用组Akt和VEGF m RNA及蛋白的表达明显升高(P<0.05或P<0.01);(2)壮骨片组与对照组相比,Akt和VEGF m RNA及蛋白表达差异无显著性意义(P>0.05);(3)与对照组比较,复方接骨片组BMP-2和Runx2 m RNA及蛋白表达升高(P<0.05);(4)与对照组相比,壮骨片组及两药联用组BMP-2及Runx2 m RNA及蛋白表达均显著增加(P<0.01);(5)结果表明,复方接骨片及壮骨片含药血清可在骨髓间充质干细胞体外分化通路中起到协同促进骨髓间充质干细胞向血管内皮细胞及成骨细胞分化的作用。
