In this paper,we study the biological response induced by heavy ions irradiation of high dose,human peripheral blood lymphocytes are irradiated in vitro by the carbon ions of LET=35 keV/μm,and the chromosome aberrati...In this paper,we study the biological response induced by heavy ions irradiation of high dose,human peripheral blood lymphocytes are irradiated in vitro by the carbon ions of LET=35 keV/μm,and the chromosome aberrations at absorbed doses of 0-20 Gy are analyzed by the calyculin A-induced premature chromosome condensation(PCC).The frequencies of PCC-rings at the stage of G2/M-phase increase steeply with radiation doses up to 20 Gy at a rate of 0.017 Gy^(-1).The G2-PCC index remains more than 5%up to 15 Gy,and 3%after 20 Gy,this is high enough to score a substantial number of chromosome spreads without dose-rate effect.The results show that the calyculin A-induced PCC technique is suitable for analyzing the chromosome damage induced by carbon ions irradiation of high dose.展开更多
The hematopoietic system in the developing mammal is highly sensitive to the damaging effects of ionizing radiation[1]. The radiation could cause serious injuries to the hematopoiesis, which give the potential health ...The hematopoietic system in the developing mammal is highly sensitive to the damaging effects of ionizing radiation[1]. The radiation could cause serious injuries to the hematopoiesis, which give the potential health risks to space travelers.Bone marrow is the most important hematopoietic organ.展开更多
The hematological effects of space radiation contribute to the compromised immune defense in astronauts observed in the space environment as well as upon landing[1]. The complications associated with the hematopoietic...The hematological effects of space radiation contribute to the compromised immune defense in astronauts observed in the space environment as well as upon landing[1]. The complications associated with the hematopoietic syndrome include infection and internal hemorrhage. The decrease in peripheral blood cell counts after radiation serves not only as a marker for the severity of the exposure, but also as a marker for treatment and prognosis[2]. In the present research, we investigated the effects of whole-body heavy-ion (80 MeV/u carbon-ion) radiation and conventional X-ray (6 MV) radiation on peripheral blood cell counts in mice model. Time effects were determined to compare the effects of the different types of radiation.展开更多
It becomes more importance to understand the toxic effects of heavy ion irradiation on normal tissue as more people spend more time in the space environment and as carbon ion radiotherapy become increasingly used in t...It becomes more importance to understand the toxic effects of heavy ion irradiation on normal tissue as more people spend more time in the space environment and as carbon ion radiotherapy become increasingly used in the treatment of cancer.Normal tissue damage due to therapeutic or accidental radiation exposure is a pervasive threat.Bone marrow,the predominant site of hematopoiesis and the differentiation of blood cells,is extremely sensitive to ionizing radiation.Irradiation with even as little as 0.1 Gy X-ray could cause a significant increase in micronucleated reticulocytes in mice.Exposure to heavy ion irradiation can have even more detrimental effects on bone marrow cells.The induction of bone marrow damage cannot be predicted correctly as the biological and molecular mechanisms altered after irradiation is still largely unknown.Identification and characterization of these molecular processes is not only imperative for the development of protective measures for cancer patients undergoing high LET radiotherapy,but also for astronauts in space.展开更多
Charged particle radiation is known to be more biologically effective than photon radiation.One example of this is the damage of human blood vessels,and this effect is an important factor influencing human health and ...Charged particle radiation is known to be more biologically effective than photon radiation.One example of this is the damage of human blood vessels,and this effect is an important factor influencing human health and is relevant to space travel as well as to cancer radiotherapy.Therefore,research on the protection endothelial cells from heavy ions radiation damage is of great significance.Studies showed that beta-D-glucan is one of the potential radioprotectors[1],but most of the studies were focused on the protection of beta-glucan on the immune and hematopoietic after X-/-rays irradiation.In the present study,we chose human umbilical endothelial cell line EA.hy926 as a model system to investigate the protective effect of beta-D-glucan on the injury induced by high-LET carbon ions irradiation,and explore the possible mechanism of the action.展开更多
Ionizing radiation is a powerful treatment modality against tumor cells,but it is not tumor specific and may affect neighboring normal cell as well.Damage to normal tissue is an obstacle to radiotherapy of cancer.Ther...Ionizing radiation is a powerful treatment modality against tumor cells,but it is not tumor specific and may affect neighboring normal cell as well.Damage to normal tissue is an obstacle to radiotherapy of cancer.Therefore,strategies to protect cancer patients from the harmful side effects of radiation without compromising the anti-cancer effects are of considerable interest in radiotherapy.Protection against ionizing radiation is also important during accidental and unavoidable exposures to radiation,such as nuclear industries,radiation accidents,space travels,radiation diagnosis,etc。展开更多
Vascular endothelial cells damage is the initial reaction of tissue radiation damage,and plays an important role in the occurrence,development and healing of irradiation damage.Vascular endothelial cells are also cons...Vascular endothelial cells damage is the initial reaction of tissue radiation damage,and plays an important role in the occurrence,development and healing of irradiation damage.Vascular endothelial cells are also considered to be an important target for radiation-induced cardiovascular disease.Therefore,research on the protection vascular endothelial cells from radiation damage is of great significance for the radiation therapy of cancer,for the protection of space astronauts health,and other nuclear radiation protection.展开更多
For several decades,lots of researchers,especially in the radiation biology,explored the mathematical models[1]which can describe the biological effects[2]well enough.However,many available mathematical models cannot ...For several decades,lots of researchers,especially in the radiation biology,explored the mathematical models[1]which can describe the biological effects[2]well enough.However,many available mathematical models cannot describe the biological effects which researchers want to know accurately.展开更多
Heavy ion irradiation attract a large interest for two applications:radiotherapy and space radiation protection in manned space missions.Exposure to heavy ions radiation results in multiple effects through DNA damage ...Heavy ion irradiation attract a large interest for two applications:radiotherapy and space radiation protection in manned space missions.Exposure to heavy ions radiation results in multiple effects through DNA damage induction.Single-cell gel electrophoresis or comet assay is known for its ability to detect DNA damage at the single cell level and has been used for years to assess DNA damage.It can detect low levels of DNA strand breaks in a short time,just using a few sample cells.DNA double strand breaks(DSBs)are measured at the neutral comet assay condition;under the alkaline comet assay condition both DNA single strand breaks(SSBs)and part DSBs are detected.The two dimensional comet assay is a modification of the two original comet assay,can simultaneously detect DNA SSBs and DSBs in the same human spermatozoa.展开更多
Among the many special factors in the space environment,space radiation especially high energy heavy ion radiation is widely regarded as one of the crucial factors that hazard the health and safety of astronaut.It is ...Among the many special factors in the space environment,space radiation especially high energy heavy ion radiation is widely regarded as one of the crucial factors that hazard the health and safety of astronaut.It is well known that biological effects of radiation are dependent on the linear-energy-transfer(LET)of the emergent beam.Heavy ions have high LET and could induce complex and clustered DNA damage,which is more difficult to be repaired than individual lesions and is even irreparable.展开更多
基金Supported by National Natural Science Foundation of China(10875153)The President Scholarship of Chinese Academy of Sciences for Postgraduate students (O906350YZ0)The Talent Training Program for PhD in West China of the Chinese Academy of Sciences(O806260XB0,and O806270XBB)
文摘In this paper,we study the biological response induced by heavy ions irradiation of high dose,human peripheral blood lymphocytes are irradiated in vitro by the carbon ions of LET=35 keV/μm,and the chromosome aberrations at absorbed doses of 0-20 Gy are analyzed by the calyculin A-induced premature chromosome condensation(PCC).The frequencies of PCC-rings at the stage of G2/M-phase increase steeply with radiation doses up to 20 Gy at a rate of 0.017 Gy^(-1).The G2-PCC index remains more than 5%up to 15 Gy,and 3%after 20 Gy,this is high enough to score a substantial number of chromosome spreads without dose-rate effect.The results show that the calyculin A-induced PCC technique is suitable for analyzing the chromosome damage induced by carbon ions irradiation of high dose.
文摘The hematopoietic system in the developing mammal is highly sensitive to the damaging effects of ionizing radiation[1]. The radiation could cause serious injuries to the hematopoiesis, which give the potential health risks to space travelers.Bone marrow is the most important hematopoietic organ.
文摘The hematological effects of space radiation contribute to the compromised immune defense in astronauts observed in the space environment as well as upon landing[1]. The complications associated with the hematopoietic syndrome include infection and internal hemorrhage. The decrease in peripheral blood cell counts after radiation serves not only as a marker for the severity of the exposure, but also as a marker for treatment and prognosis[2]. In the present research, we investigated the effects of whole-body heavy-ion (80 MeV/u carbon-ion) radiation and conventional X-ray (6 MV) radiation on peripheral blood cell counts in mice model. Time effects were determined to compare the effects of the different types of radiation.
基金National Natural Science Foundation of China(11575259)。
文摘It becomes more importance to understand the toxic effects of heavy ion irradiation on normal tissue as more people spend more time in the space environment and as carbon ion radiotherapy become increasingly used in the treatment of cancer.Normal tissue damage due to therapeutic or accidental radiation exposure is a pervasive threat.Bone marrow,the predominant site of hematopoiesis and the differentiation of blood cells,is extremely sensitive to ionizing radiation.Irradiation with even as little as 0.1 Gy X-ray could cause a significant increase in micronucleated reticulocytes in mice.Exposure to heavy ion irradiation can have even more detrimental effects on bone marrow cells.The induction of bone marrow damage cannot be predicted correctly as the biological and molecular mechanisms altered after irradiation is still largely unknown.Identification and characterization of these molecular processes is not only imperative for the development of protective measures for cancer patients undergoing high LET radiotherapy,but also for astronauts in space.
文摘Charged particle radiation is known to be more biologically effective than photon radiation.One example of this is the damage of human blood vessels,and this effect is an important factor influencing human health and is relevant to space travel as well as to cancer radiotherapy.Therefore,research on the protection endothelial cells from heavy ions radiation damage is of great significance.Studies showed that beta-D-glucan is one of the potential radioprotectors[1],but most of the studies were focused on the protection of beta-glucan on the immune and hematopoietic after X-/-rays irradiation.In the present study,we chose human umbilical endothelial cell line EA.hy926 as a model system to investigate the protective effect of beta-D-glucan on the injury induced by high-LET carbon ions irradiation,and explore the possible mechanism of the action.
文摘Ionizing radiation is a powerful treatment modality against tumor cells,but it is not tumor specific and may affect neighboring normal cell as well.Damage to normal tissue is an obstacle to radiotherapy of cancer.Therefore,strategies to protect cancer patients from the harmful side effects of radiation without compromising the anti-cancer effects are of considerable interest in radiotherapy.Protection against ionizing radiation is also important during accidental and unavoidable exposures to radiation,such as nuclear industries,radiation accidents,space travels,radiation diagnosis,etc。
文摘Vascular endothelial cells damage is the initial reaction of tissue radiation damage,and plays an important role in the occurrence,development and healing of irradiation damage.Vascular endothelial cells are also considered to be an important target for radiation-induced cardiovascular disease.Therefore,research on the protection vascular endothelial cells from radiation damage is of great significance for the radiation therapy of cancer,for the protection of space astronauts health,and other nuclear radiation protection.
文摘For several decades,lots of researchers,especially in the radiation biology,explored the mathematical models[1]which can describe the biological effects[2]well enough.However,many available mathematical models cannot describe the biological effects which researchers want to know accurately.
文摘Heavy ion irradiation attract a large interest for two applications:radiotherapy and space radiation protection in manned space missions.Exposure to heavy ions radiation results in multiple effects through DNA damage induction.Single-cell gel electrophoresis or comet assay is known for its ability to detect DNA damage at the single cell level and has been used for years to assess DNA damage.It can detect low levels of DNA strand breaks in a short time,just using a few sample cells.DNA double strand breaks(DSBs)are measured at the neutral comet assay condition;under the alkaline comet assay condition both DNA single strand breaks(SSBs)and part DSBs are detected.The two dimensional comet assay is a modification of the two original comet assay,can simultaneously detect DNA SSBs and DSBs in the same human spermatozoa.
文摘Among the many special factors in the space environment,space radiation especially high energy heavy ion radiation is widely regarded as one of the crucial factors that hazard the health and safety of astronaut.It is well known that biological effects of radiation are dependent on the linear-energy-transfer(LET)of the emergent beam.Heavy ions have high LET and could induce complex and clustered DNA damage,which is more difficult to be repaired than individual lesions and is even irreparable.
