AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine,theplasmi...AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine,theplasmid could be expressed in eukaryotic cellswhen injected into Wistar rats by intravenousroute.At different time intervals after the injection,the distribution and expression of the plasmid inliver of rats were observed and compared using insitu hybridization and immunohistochemistry.RESULTS The expression of the plasmid bindingto liposomes or G-PLL could be markedly observed24 h later,and began to decrease one week later,but it still could be observed up to three weeks.Both liposomes and G-PLL could deliver theplasmid to the liver effectively,but the effect of thelatter was better than the former concerning thedistribution and expression of the plasmid targeteduptake in the liver.CONCLUSION G-PLL is better than liposome asthe targeted carrier for delivering exogenous genesto the liver.展开更多
Shear velocity is an important index for evaluating soil dynamic properties of the site. In this paper, the statistical relation of shear velocity with soil properties is set up by statistical analysis of shear veloci...Shear velocity is an important index for evaluating soil dynamic properties of the site. In this paper, the statistical relation of shear velocity with soil properties is set up by statistical analysis of shear velocities and relevant soil properties measured in Tianjin and Shanghai. The obtained formula has some referential value for the evaluation and classification of the site in offshore areas of China.展开更多
By using Morse potential model, we calculate the temperature dependence of critical resolved shear stress (CRSS) of some single crystals. Dislocation-phonon interaction is considered in this paper. Theoretical calcula...By using Morse potential model, we calculate the temperature dependence of critical resolved shear stress (CRSS) of some single crystals. Dislocation-phonon interaction is considered in this paper. Theoretical calculations fit experimental law well. Investigations show: interatomic force is the physical intrinsic that controls the plastic deformation processing of materials, and the temperature dependence of CRSS mainly comes from the temperature dependence of dislocation vibration.展开更多
基金the National Natural Science Foundation of China(№39570336).
文摘AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine,theplasmid could be expressed in eukaryotic cellswhen injected into Wistar rats by intravenousroute.At different time intervals after the injection,the distribution and expression of the plasmid inliver of rats were observed and compared using insitu hybridization and immunohistochemistry.RESULTS The expression of the plasmid bindingto liposomes or G-PLL could be markedly observed24 h later,and began to decrease one week later,but it still could be observed up to three weeks.Both liposomes and G-PLL could deliver theplasmid to the liver effectively,but the effect of thelatter was better than the former concerning thedistribution and expression of the plasmid targeteduptake in the liver.CONCLUSION G-PLL is better than liposome asthe targeted carrier for delivering exogenous genesto the liver.
文摘Shear velocity is an important index for evaluating soil dynamic properties of the site. In this paper, the statistical relation of shear velocity with soil properties is set up by statistical analysis of shear velocities and relevant soil properties measured in Tianjin and Shanghai. The obtained formula has some referential value for the evaluation and classification of the site in offshore areas of China.
文摘By using Morse potential model, we calculate the temperature dependence of critical resolved shear stress (CRSS) of some single crystals. Dislocation-phonon interaction is considered in this paper. Theoretical calculations fit experimental law well. Investigations show: interatomic force is the physical intrinsic that controls the plastic deformation processing of materials, and the temperature dependence of CRSS mainly comes from the temperature dependence of dislocation vibration.
